1,357 research outputs found
in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis
No full text: Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte glycoprotein (MOG) requires immunization by a MOG peptide emulsified in complete Freund's adjuvant (CFA) containing inactivated Mycobacterium tuberculosis. The antigenic components of the mycobacterium activate dendritic cells to stimulate T-cells to produce cytokines that promote the Th1 response via toll-like receptors. Therefore, the amount and species of mycobacteria present during the antigenic challenge are directly related to the development of EAE. This methods paper presents an alternative protocol to induce EAE in C57BL/6 mice using a modified incomplete Freund's adjuvant containing the heat-killed Mycobacterium avium subspecies paratuberculosis strain K-10. M. paratuberculosis, a member of the Mycobacterium avium complex, is the causative agent of Johne's disease in ruminants and has been identified as a risk factor for several human T-cell-mediated disorders, including multiple sclerosis. Overall, mice immunized with Mycobacterium paratuberculosis showed earlier onset and greater disease severity than mice immunized with CFA containing the strain of M. tuberculosis H37Ra at the same doses of 4 mg/mL. The antigenic determinants of Mycobacterium avium subspecies paratuberculosis (MAP) strain K-10 were able to induce a strong Th1 cellular response during the effector phase, characterized by significantly higher numbers of T-lymphocytes (CD4+ CD27+), dendritic cells (CD11c+ I-A/I-E+), and monocytes (CD11b+ CD115+) in the spleen compared to mice immunized with CFA. Furthermore, the proliferative T-cell response to the MOG peptide appeared to be highest in M. paratuberculosis-immunized mice. The use of an encephalitogen (e.g., MOG35-55) emulsified in an adjuvant containing M. paratuberculosis in the formulation may be an alternative and validated method to activate dendritic cells for priming myelin epitope-specific CD4+ T-cells during the induction phase of EAE
The association of kyphosis assessed in supine and standing positions with future activities of daily living dependence: The Kurabuchi Study
No full text主査 : 武者芳朗 / タイトル : The association of kyphosis assessed in supine and standing positions with future activities of daily living dependence: The Kurabuchi Study / 著者 : Yuichiro Yokoyama, Yuji Nishiwaki, Takehiro Michikawa, Haruhiko Imamura, Takahiro Nakamura, Toru Takebayashi, Hiroshi Takahashi / 掲載誌 : Archives of Osteoporosis /巻号・発行年等 : 12(1):105, 201
Pharmacological characterization of microminipig as a model to assess the drug-induced cardiovascular responses for non-clinical toxicity and/or safety pharmacology studies.
No full text主査 : 赤坂喜清 / タイトル : Pharmacological characterization of microminipig as a model to assess the drug-induced cardiovascular responses for non-clinical toxicity and/or safety pharmacology studies. /著者 : Hirofumi Yokoyama, Yuji Nakamura, Hiroyuki Saito, Yukitoshi Nagayama,Kiyotaka Hoshiai, Takeshi Wada, Hiroko Izumi-Nakaseko, Kentaro Ando,Yasuki Akie, Atsushi Sugiyama /掲載誌 : The Journal of Toxicological Sciences /巻号・発行年等 : 42(1):93-101,2017 /本文ファイル: 出版者
The association of kyphosis assessed in supine and standing positions with future activities of daily living dependence: The Kurabuchi Study
No full text主査 : 武者芳朗 / タイトル : The association of kyphosis assessed in supine and standing positions with future activities of daily living dependence: The Kurabuchi Study / 著者 : Yuichiro Yokoyama, Yuji Nishiwaki, Takehiro Michikawa, Haruhiko Imamura, Takahiro Nakamura, Toru Takebayashi, Hiroshi Takahashi / 掲載誌 : Archives of Osteoporosis /巻号・発行年等 : 12(1):105, 2017 / 本文ファイル: 査読後原稿 / The final publication is available at Springer via http://dx.doi.org/10.1007/s11657-017-0401-
Pharmacological characterization of microminipig as a model to assess the drug-induced cardiovascular responses for non-clinical toxicity and/or safety pharmacology studies.
No full text主査: 赤坂 喜清 /タイトル: Pharmacological characterization of microminipig as a model to assess the drug-induced cardiovascular responses for non-clinical toxicity and/or safety pharmacology studies. /著者: Hirofumi Yokoyama, Yuji Nakamura, Hiroyuki Saito, Yukitoshi Nagayama, Kiyotaka Hoshiai, Takeshi Wada, Hiroko Izumi-Nakaseko, Kentaro Ando, Yasuki Akie, Atsushi Sugiyama /掲載誌: The Journal of Toxicological Sciences /巻号・発行年等: 42(1):93-101,201
Comparing clinical and imaging features of patients with MOG antibody-positivity and with and without oligoclonal bands
No full textIntroduction: Myelin-oligodendrocyte glycoprotein antibody (MOG)-associated disorder (MOGAD) is a recently identified immune-mediated inflammatory disorder of the central nervous system (CNS). The significance of oligoclonal bands (OCBs) is not fully elucidated. This study investigated the clinical differences between patients with MOGAD who tested positive or negative for OCBs. Methods: The study was conducted on 23 patients with MOG-IgG-seropositivity who presented with central nervous system (CNS) symptoms. The patients were screened and divided into OCB-positive (n=10) and OCB-negative (n=13) groups, and their demographic, clinical, and magnetic resonance imaging (MRI) features were compared. Results: The results revealed that patients with OCB-positivity had a significantly higher frequency of relapse, and their IgG index was significantly higher. Discussion: OCBs were common in MOGAD met the consensus criteria. The study concluded that careful treatment decision-making is necessary in MOG antibody-positive cases with OCB-positivity
Anti-Mycobacterial Antibodies in Paired Cerebrospinal Fluid and Serum Samples from Japanese Patients with Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder
No full textLocal synthesis of antibodies and presence of oligoclonal bands in the cerebrospinal fluid (CSF) are hallmarks of multiple sclerosis (MS). We investigated the frequency of antibodies against mycobacterial and relevant human epitopes in the CSF of patients with MS or neuromyelitis optica spectrum disorder (NMOSD) and whether these antibodies differed from those present in the serum. Matched serum and CSF samples from 46 patients with MS, 42 patients with NMOSD, and 29 age-matched and sex-matched control subjects were screened retrospectively for the presence of antibodies against Mycobacterium avium subsp. paratuberculosis (MAP) pentapeptide (MAP_5p), MAP_2694295–303, and myelin basic protein (MBP)85–98 peptides by using indirect ELISA. Serum levels of anti-MAP_5p and anti-MAP_2694295–303 antibodies were highly prevalent in patients with MS when compared to patients with NMOSD and controls. Several patients with MS had detectable anti-MAP_5p and anti-MAP_2694295–303 antibodies in the CSF. Furthermore, a group of patients with MS showed intrathecally restricted production of antibodies against these peptides. Women appeared to mount a stronger humoral response to mycobacterial peptides than men. No significant difference in the frequency of anti-MBP85–98 antibodies was found between patients with MS and those with NMOSD. These data highlight the zoonotic potential of MAP, which suggests its involvement in MS etiopathogenesis
Datation d'ossements et de planchers stalagmitiques provenant de L'Abri-des-Pêcheurs (Ardèche) par la méthode des déséquilibres des familles de l'uranium (U-Th) [ Uranium series dating of stalagmitic floors and hones samples front Abri-des-Pêcheurs (Ardèche-France). ]
No full textUranium-series disequilibrium dating of bones and speleothems taken from the moustenan levels of the «Abri des Pêcheurs» (Casteljau, Ardèche) allowed to give some chronological guide-marks and showed that the age of these levels ranges between 39000 and 110000 years.La datation par la méthode des déséquilibres des familles de l'uranium (234U/230Th) d'ossements et de planchers stalagmitiques provenant des niveaux mousténens de l'Abri des Pêcheurs (Casteljau, Ardèche) a permis de donner des points de repère géochronologiques et montre que l'âge de ces niveaux est compris entre 39000 ans et 110000 ans.Masaoudi Hassan, Falguères Christophe, Bahain Jean Jacques, Yokoyama Yuji, Lhomme Gilbert. Datation d'ossements et de planchers stalagmitiques provenant de L'Abri-des-Pêcheurs (Ardèche) par la méthode des déséquilibres des familles de l'uranium (U-Th) [ Uranium series dating of stalagmitic floors and hones samples front Abri-des-Pêcheurs (Ardèche-France). ]. In: Quaternaire, vol. 5, n°2, 1994. pp. 79-83
Humoral response against host-mimetic homologous epitopes of Mycobacterium avium subsp. Paratuberculosis in Japanese multiple sclerosis patients
No full textSeveral works have demonstrated the existence of a link between Mycobacterium avium subsp. Paratuberculosis (MAP) and MS in Italy. In this study, we analyzed the serology of MAP in a Japanese population while looking at several markers of MAP. Fifty MS patients, 12 clinically isolated syndrome (CIS) patients, 30 other neurological disorders (OND) patients, and 50 healthy controls (HCs) were tested using ELISA for the presence of IgG antibodies toward immunodominant epitopes MAP-0106c121-132, homologues MBP85-98, homologues IRF5424-432, MAP-402718-32, and MAP-2694295-303. MAP-positive patients were also analyzed in relation to their clinical/demographic characteristics. Amongst all peptides, only antibodies against MAP-2694295-303 were more prevalent in MS patients (30%), as compared to OND patients (3%) (p = 0.009; area under roc curve (AUC) = 0.61) and HCs (2%) (p = 0.0004; AUC = 0.65) and in CIS patients (25%) compared to HCs (p = 0.023; AUC = 0.55). Logistic regression analysis showed a higher frequency of anti-MAP-2694295-303 antibodies in the sera of oligoclonal bands positive MS patients (p = 0.2; OR = 2, 95%CI: 0.55-7.7). These findings support the view that MAP could act as a risk factor or a triggering agent of MS in some Japanese patients with a genetic susceptibility to the mycobacterium
Impacts of surgically performed renal denervation on the cardiovascular and electrophysiological variables in the chronic atrioventricular block dogs: Comparison with those of amiodarone treatment
No full text主査: 池田 隆徳 /タイトル: Impacts of surgically performed renal denervation on the cardiovascular and electrophysiological variables in the chronic atrioventricular block dogs: Comparison with those of amiodarone treatment /著者: Takeshi Wada, Hiroshi Ohara, Yuji Nakamura, Hirofumi Yokoyama, Xin Cao, Hiroko Izumi-Nakaseko, Kentaro Ando, Nobuyuki Murakoshi, Akira Sato, Kazutaka Aonuma, Akira Takahara, Yuji Nakazato, Atsushi Sugiyama /掲載誌: Circulation Journal /巻号・発行年等: 80(7):1556-1563, 2016
- …
