1,721,257 research outputs found
supplementary_materials - Social Class and Children’s Prosociality: A Study in the Context of China’s Dual Urban–Rural Structure
No full textsupplementary_materials for Social Class and Children’s Prosociality: A Study in the Context of China’s Dual Urban–Rural Structure by Yi Kuang, Fei Wang and Zuo-Jun Wang in Social Psychological and Personality Science</p
Replication Data for: Long-term trends of Nordic power market: A review
No full textThis dataset contains collections of key parameters in the Nordic power market outlooks from 43 scenarios in 15 reports published between 2016 and 2019. The key parameters include fuel prices, carbon prices, electricity consumption, installed capacities, wind generation, and power price. All data are extracted directly from the material and converted to the same unit when necessary
Real-Time Protein Monitoring and Manipulation Using Total Internal Reflection Fluorescence Microscopy
No full text自從90 年代後期人類基因計劃解出大量基因體資料，以及電腦處理資訊速度大為發展，使得蛋白質可以被快速測定。蛋白質結構、功能性與其分子間的作用，都是蛋白質體研究裡相當重要的課題。
本文首次成功地觀測與分析抗原分子產生的動態行為和其與抗體分子間作用，並量測到其動態運動三度空間的位置與速度表現，此乃是藉著整合全反射螢光顯微術與微機電製程技術，進行蛋白質在物性環境改變時，。利用全反射光場以漸逝波激發螢光，使全反射螢光顯微術具有離玻璃基材150至700奈米尺寸等級以內的觀測功能，並且其影像的訊號背景比遠高於其他的顯微技術；而在橫向尺寸的功能也藉著量測1微米的螢光粒子得到了驗證。另外，配合速度每秒30張畫面的影像擷取系統，可對標定螢光之抗原分子Anti-IgG，進行即時偵測了解其從運動至與抗體鍵結時之狀態，並加以分析。
利用微機電製程技術，設計並製作整合氧化銦錫 (ITO)透明電極與PDMS微流道之生物晶片，為了達到尺寸微小化(1.6與0.8微升)，且能與符合目前商用蛋白質生物晶片之規格，與蛋白質分子IgG和Anti-IgG相容。藉此在單分子偵測上，本文首次利用全反射螢光顯微鏡即時觀察到在固液相單一抗原分子Anti-IgG結合於抗體的狀態(1張/30秒)；同時也觀察到分子在流體邊界層運動行為，並成功地追蹤並分析抗原分子在流體邊界層的三度空間運動軌跡(空間範圍間距：X軸 14.7微米；Y軸 1.3微米；Z軸 0.24微米)與速度表現，與流體理論作驗證，發現反應物分子(抗原)擴散受到流體邊界層所影響。此外，藉著生物晶片以施加直流電的方式操控奈米粒子，實際達到操控粒子的運動，觀測粒子的運動狀態，並發現電壓與粒子運動速度呈線性增長，將有助於未來在蛋白質分子間結合與解離作用的研究。
由於生物分子領域上仍有許多未知的現象，全反射螢光顯微術與微機電製程技術在單分子影像之觀測將扮演重要的角色。以了解生物分子在生物晶片上之表面特性與流體邊界層在晶片表面對生物分子之擴散的影響，以利未來在研究上或是奈米生物技術上之發展潛力。The study of biomolecular recognition has been becoming crucial to provide insights into molecular genetics, design of biosensor devices, drug design, and development of targeted drug delivery systems. The in-depth understanding of biomolecular recogntion involves adsorption, interaction and desorption as well as associated manipulation between biomolecules. In this present study, we have successfully demonstrated a single biomolecular detection and real-time tracking of anti-IgG in a microchannel using total internal reflection flurorescence (TIRF) microscopy for illustration of protein adsorption and recognition.
TIRF microscopy is a well-suited technique for real-time imaging and monitoring of a single protein molecule in nano-layer fluidics due to its unique evanencent wave at the optically index-mismatch interaface that may excites fluorescences at the transparent near-wall region. Recent advances in charge coupled device (CCD) camera detection efficiency and speed have enabled the microscopy of temporal and spatial resolutions to be far-reaching 0.033 ms and 0.3 μm, respectively. A modified inverted TIRF microscopy was newly established, which allows a directed laser beam underneath through the inverted microscopy to be incident in a critical angle into the surface inside the microchannel. In this microchannel, the conductive ITO film was deposited to be electrically feedthrough for electrical manipulation. Based on the fluorescent beads analysis, which are 1.1 μm in size, the capability of TIRFM for single molecules monitoring and tracking, even the measurement in lateral size of molecules was demonstrated.
In this study, the TIRFM incorporates a MEMS-based electrical control biochip to monitor and track the motion of a single antigen molecule in which the real-time position and velocity of each frames were tracked and measured. The motion of an antigen molecule was founded to be dominated in a hydrodynamic boundary layer. A further comparison of experimental results with theoretics appears to be deviated unexpectedly, which provokes a further thought that a nano-layer fluidics exhibits a novel non-classical fluidic characteristics.
At last, the motion of fluorescence nanobeads manipulated by the application of DC voltage was real-time monitored. The velocities of beads were shown to be in a linear accordance with the applied voltages. As a result of the accordance, the manipulation of nonobeads with electrical control was demonstrated and verified.中文摘要 1
Abstract 3
致謝 5
目錄 7
圖表目錄 10
表格目錄 13
第 1 章 緒論 14
1-1 前言 14
1-1-1 蛋白質即時影像偵測 14
1-1-2 蛋白質分子操控 15
1-1-3 全反射螢光顯微技術與微機電技術 15
1-2 研究動機 16
1-3 文獻回顧 18
1-3-1 單分子偵測 18
1-3-2 細胞運輸泡偵測 20
1-3-3 與其他技術結合應用 21
1-4 研究方法 23
1-5 章節提要 24
第 2 章 全反射螢光顯微鏡原理與架設 25
2-1 全反射螢光顯微鏡之基本原理 25
2-1-1 漸逝波[11] 26
2-1-2 螢光強度之計算 28
2-1-3 中間層 29
2-2 全反射螢光顯微鏡之光學架設 30
2-2-1 螢光染劑 31
2-2-2 整體光學架構 34
2-2-3 數值孔徑 36
2-2-4 螢光濾鏡組 39
2-2-5 螢光影像擷取系統 41
2-3 全反射螢光顯微鏡之特性與應用 43
2-3-1 穿透深度 43
2-3-2 訊號背景比 44
2-3-3 即時影像觀測 45
第 3 章 電控式生物晶片設計與製作 47
3-1 電控式生物晶片之設計理念 47
3-1-1 文獻回顧 47
3-1-2 抗原－抗體之鍵結 49
3-1-3 專一性結合作用 51
3-1-4 抗原－抗體結合作用之可逆性 52
3-1-5 動力常數 54
3-1-6 質量運送效應[25] 56
3-1-7 電控式生物晶片之設計 58
3-2 電控式生物晶片之製備方法 60
3-2-1 氧化銦錫電極之製程 60
3-2-2 微流道之製程 61
3-2-3 生物分子固定化 65
第 4 章 實驗結果與分析 68
4-1 微奈米粒子之即時偵測與分析 68
4-1-1 訊號背景比 68
4-1-2 橫向尺寸量側 71
4-2 蛋白質單分子即時偵測之定性分析 75
4-2-1 抗體與抗原結合反應之單分子偵測 75
4-2-2 微粒子操控與即時偵測 80
第 5 章 結論與未來展望 83
第 6 章 參考資料 8
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Association Between Epilepsy and Erectile Dysfunction: Evidence from a Population-Based Study
No full textIntroduction. Although the association between epilepsy and sexual disorders has long been known, very few studies have attempted to explore the association between epilepsy and erectile dysfunction (ED). Aim. This population-based case-control study aimed to investigate the association between ED and a prior diagnosis with epilepsy. Methods. This study used data from the Longitudinal Health Insurance Database 2000 in Taiwan. The cases comprised 6,427 patients with a diagnosis of ED and 32,135 controls who were frequency matched with cases based on 10-year age groups and index year. Conditional logistic regression was used to calculate the odds ratios (ORs) for prior epilepsy. Main Outcome Measure. The prevalence and risk of epilepsy between cases and controls. Results. Of the sampled patients, 3,861 (14%) were diagnosed before the index date: 1,358 individuals (19.7%) were cases and 2,503 (12.1%) were controls. After adjusting for hypertension, diabetes, hyperlipidemia, renal disease, coronary heart disease, obesity, alcohol abuse/alcohol dependence syndrome, and socioeconomic status, conditional logistic regression analysis revealed that patients with ED were more likely to have been diagnosed with prior epilepsy than controls (OR = 1.83, 95% CI = 1.512.21). Compared with controls, the adjusted ORs for prior generalized epilepsy and partial epilepsy for cases were 2.13 (95% CI = 1.523.00) and 1.64 (95% CI = 1.312.06), respectively. The most pronounced associations were detected in ED cases aged between 30 and 39 who were 3.04 (95% CI = 1.675.50) times more likely than controls to have been previously diagnosed with epilepsy. Conclusions. Our findings suggest a positive correlation between ED and a prior diagnosis with epilepsy. Keller J, Chen Y-K, and Lin H-C. Association between epilepsy and erectile dysfunction: Evidence from a population-based study. J Sex Med 2012;9:22482255
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