1,720,953 research outputs found
Investigating Tangeretin Targeted Pathway by Chemical-Genetic Interaction in Yeast
No full text多氧甲基黃酮 (polymethoxyflavones, PMFs) 為柑橘類果皮中含多氧甲基的酚類物質，目前已知其具有廣泛的生理活性，包括抗癌與抗發炎等。先前有許多研究探討PMFs與水解PMFs的抗癌活性，唯目前為止，其在細胞中的作用目標仍不明確。因此，我們利用化合物-基因協調作用的概念，以酵母菌作為研究平台，發掘PMFs在細胞中的可能作用目標。研究顯示，PMFs維持基因穩定上無顯著作用，顯示PMFs不是透過提升細胞的DNA修復因子來達到其生理活性。利用化合物對酵母菌DNA修復基因缺失的突變株作篩選，我們發現sgs1基因突變株在色胺酸被限制的情況下對PMFs成員之一- 橘皮素 (tangeretin) 敏感，無論是在生長速度或是存活率被顯著被抑制。另外，sgs1突變菌株在橘皮素的介入下，其細胞週期會停滯在G1 / S phase，並提升了γH2A的量。透過基因晶片，我們發現橘皮素會抑制sgs1突變株之RNA合成相關的基因的表現量，並提高其含氮化合物與胺基酸的合成基因之表現。透過sgs1突變株對放射菌酮的測試，發現橘皮素並無直接抑制蛋白質合成的效果，反而卻拯救了sgs1與slx4為主的雙股修復相關基因突變株在色氨酸酸剔除或橘皮素介入下的敏感性。結果推測，橘皮素可能是透過抑制sgs1突變株的rRNA的形成，在色胺酸被限制的情況下，間接導致蛋白質合成不利，進而促使菌株死亡。此研究顯示橘皮素在抗癌途徑的可能機制，並發現胺基酸與DNA雙股斷裂修復過程可能存在某種互動關係，唯需進一步實驗驗證。Polymethoxyflavones (PMFs) is a dietary phytochemical and they have been widely investigated in the field of inflammatory and anti-carcinogenic with proven bioactivities but they actual functioning target in cells remain unclear. Here, we used the model of chemical-genetic interaction model, with budding yeast as platform, to screen the potential targets or pathways involved by PMFs. We found out that SGS1 gene deleted yeast is sensitive to tangeretin, one of a member of PMFs. sgs1 defective yeast showed lower growth rate, survival rate, delayed G1 and S phase and elevated γH2A level in cell cycle under tryptophan drop-out condition. Through microarray, we found out that tangeretin is potential to inhibit expression of RNA progression related genes and up-regulated nitrogen compounds and amino acid synthesis pathways of sgs1 defective cells. Data also showed that tangeretin is incapable to inhibit protein synthesis directly as cycloheximide do. Interestingly, cycloheximide rescued the sensitive- phenotype of sgs1 and slx4 defective cells under tryptophan depletion and tangeretin involved condition. Our results showed tangeretin is potential to suppress rRNA precession of sgs1 defective cells and resulting improper protein synthesis under tryptophan depletion condition. This result demonstrated the potential pathway of anti-carcinogenic effect played by tangeretin. Additionally, we also observed the potential of availability of relationship between amino acid balances and DNA double strains break repair pathways. However, further investigation is required for this observation
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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