584,982 research outputs found
Participation of c-FLIP in NLRP3 and AIM2 inflammasome activation
No full textCellular FLICE-inhibitory protein (c-FLIP) is an inhibitor of caspase-8 and is required for macrophage survival. Recent studies have revealed a selective role of caspase-8 in noncanonical IL-1 beta production that is independent of caspase-1 or inflammasome. Here we demonstrated that c-FLIPL is an unexpected contributor to canonical inflammasome activation for the generation of caspase-1 and active IL-1 beta. Hemizygotic deletion of c-FLIP impaired ATP-and monosodium uric acid (MSU)-induced IL-1 beta production in macrophages primed through Toll-like receptors (TLRs). Decreased IL-1 beta expression was attributed to a reduced activation of caspase-1 in c-FLIP hemizygotic cells. In contrast, the production of TNF-alpha was not affected by downregulation in c-FLIP. c-FLIPL interacted with NLRP3 or procaspase-1. c-FLIP is required for the full NLRP3 inflammasome assembly and NLRP3 mitochondrial localization, and c-FLIP is associated with NLRP3 inflammasome. c-FLIP downregulation also reduced AIM2 inflammasome activation. In contrast, c-FLIP inhibited SMAC mimetic-, FasL-, or Dectin-1-induced IL-1 beta generation that is caspase-8-mediated. Our results demonstrate a prominent role of c-FLIPL in the optimal activation of the NLRP3 and AIM2 inflammasomes, and suggest that c-FLIP could be a valid target for treatment of inflammatory diseases caused by over-activation of inflammasomes
Digital-twin-based active input refinement for insertion loss estimation and QoT optimization in C and C + L networks
Full text linkQuality of transmission (QoT) prediction is a fundamental function in optical networks. It is typically embedded within a digital twin and used for operational tasks, including service establishment, service rerouting, and (per-channel or per-amplifier) power management to optimize the working point of services and hence to maximize their capacity. Inaccuracy in QoT prediction results in additional, unwanted design margins. A key contributor to QoT inaccuracy is the uncertain knowledge of fiber insertion loss, e.g., the attenuation due to connector losses at the beginning or at the end of each fiber span, as such loss cannot be directly monitored. Indeed, insertion losses drive the choice of the launch power in fiber spans, which in turn drive key physical effects, including the Kerr and stimulated Raman scattering (SRS) effects, which affect services’ QoT. It is thus important to estimate (and detect possibly anomalous) fiber insertion losses at each span. We thereby propose a novel active input refinement (AIR) technique using active probing to estimate insertion losses in C and C
L systems. Here, active probing consists of adjusting amplifier gains span by span to slightly alter SRS. The amount of adjustment must be sufficient to be measurable (such that insertion losses can be inferred from the measures) but small enough to have a negligible impact on running services in a live network. The method is validated by simulations on a European network with 30 optical multiplex sections (OMSs) in C and C
L configurations and by lab experiments on a C-band network, demonstrating that AIR significantly improves insertion loss estimation, network QoT optimization, and QoT prediction compared with other state-of-the-art monitoring techniques. This work underscores the critical role of accurate estimation of QoT inputs in enhancing optical network performance
Infrared properties of single crystal SrTiO3, a substrate for high temperature superconducting films
No full textElectrochemical Detection of High-Sensitivity C-Reactive Protein Based on Biomimic Design of Electroactive Nanoassembly Multilayers
No full textIn this study, the gold electrode was modified with a ferrocene-terminated alkanethiol and phospholipid complex layer, which is designed and fabricated to serve as a C-reactive protein sensor using electrochemical determination. The scope of this research is to know whether a ferrocene-terminated self-assembled monolayer and hydrogenated phosphocholine hybrid bilayer could be used to perform C-reactive protein detection or not. After a series of experiments, the result shows that mixed electroactive SAM can facilitate electrons transferring from the solution to the electrode. And after coating phospholipids, this phenomenon seems to be hindered from the electrode. But this provoked small electrical signal of the recognition layer still allows for further usage. According to the result, it can be used to measure C-reactive protein and its electrochemical property and the changes of the electrode's electron transfer ability are characterized by cyclic voltammetry. This study demonstrates self-assembled ferrocene-terminated alkanethiol and phospholipid complex structure has potential as a C-reactive protein sensor and is stable to detect in the aqueous phase.補正完
Molecular structure of highly excited resonant states in Mg-24 and the corresponding Be-8+O-16 and C-12+C-12 decays
No full textExotic Be-8 and C-12 decays from high-lying resonances in Mg-24 are analyzed in terms of a cluster model. The calculated quantities agree well with the corresponding experimental data. It is found that the calculated decay widths are very sensitive to the angular momentum carried by the outgoing cluster. It is shown that this property makes cluster decay a powerful tool to determine the spin as well as the molecular structures of the resonances.Physics, NuclearSCI(E)7ARTICLE5null8
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
E-commerce application With Angular and C#
No full textAn E-commerce web project using C# has been developed. Ecommerce is the buying and selling of goods and services over the Internet. This project was developed under the concept of a company that sells various types of furiture.In order to create this application, ASP.NET Core, Angular were used for back-end and front-end respectively.Visual Studio Code was used for code editing. SQLite, Redis, and serveral packages were installed. To support this project, In addition, Bootstrap for simplifying the development of the web pages and Stripe for taking payment were used.BSc/BAComputer Scienc
Shortcourse Visual C++ 2013
No full textVisual C++ adalah produk IDE (Integrated Development Environment) untuk bahasa pemrograman C dan C++ yang dikembangkan oleh Microsoft. Visual C++ merupakan salah satu bagian dari paket Microsoft Visual Studio. Bahasa pemrograman ini memiliki kelebihan dalam bahasa yang fleksibel karena Visual C++ memiliki tipe data yang banyak serta dapat mengatur pengalokasian memori dengan baik. Adanya Visual C++ pointer adalah kelebihan bahasa C++ yang tidak dimiliki oleh bahasa lain. Saat ini Visual C++ terbaru adalah versi 2013. Versi ini telah mendukung C++11 dan C99.Buku ShortCourse (SC): Visual C++ 2013 ini sangat tepat digunakan sebagai panduan untuk menguasai aplikasi Visual C++ 2013. Dengan bahasa yang rapi dan mudah dipahami dijamain pembaca dapat langsung praktik untuk membuat program menggunakan Visual C++ 2013.Buku ini akan membahas :- Pendahuluan- Mengenal pengindentifikasi variabel dan tipe data- Mengenal operator- Mengenal kondisi dan pengulangan- Mengenal Array String dan Pointer- Pemrograman berbasis windows- Mengakses data sourcexii + 276 hlm.; 16 x 23 cm
B -> eta K-c(eta ' K-c) decays in QCD factorization
No full textWe study the exclusive decays of the B meson into pseudoscalar charmonium states eta(c) and eta(c)' within the QCD factorization approach and find that the non-factorizable corrections to naive factorization are infrared safe at leading-twist order. The spectator interactions arising from the kaon twist-3 effects are formally power suppressed but chirally and logarithmically enhanced. An important improvement by including the O(alpha(s)) corrections is the cancellation of the renormalization scale mu dependence of the decay amplitude. However, the calculated decay rates are too small to accommodate the experimental data. On the other hand, we compare the theoretical calculations for B meson decays to J/psi, psi', eta(c) and eta'(c), and find that the predicted relative decay rates of these four states are approximately compatible with the experimental data.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000223097800007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Physics, Particles & FieldsSCI(E)17ARTICLE3365-3703
Tumor necrosis factor-related apoptosis-inducing ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells.
No full textTumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can trigger apoptosis in some tumor cells but not other tumor cells. To explore the signal transduction events in TRAIL-triggered apoptosis and its modulation in nontransfected tumor cells, we analyzed TRAIL-induced death-inducing signaling complex (DISC) in TRAIL-sensitive and -resistant glioma cells. Caspase-8 and caspase-10 were recruited to the DISC, where they were proteolytically activated to initiate apoptosis in TRAIL-sensitive glioma cells. Caspase-8 and caspase-10 were also recruited to the DISC in TRAIL-resistant cells, but their further activation was inhibited by two antiapoptotic proteins termed cellular Fas-associated death domain-like interleukin-1beta-converting enzyme-inhibitory protein (c-FLIP) and phosphoprotein enriched in diabetes/phosphoprotein enriched in astrocytes-15kDa (PED/PEA-15). Both long and short forms of c-FLIP were recruited to the DISC, where the long form c-FLIP was cleaved to produce intermediate fragments. Of the three isoforms of PED/PEA-15 proteins, only the doubly phosphorylated form was expressed and recruited to the DISC in TRAIL-resistant cells, indicating that the phosphorylation status of PED/PEA-15 determines its recruitment in the cells. Treatment with calcium/calmodulin-dependent protein kinase inhibitor rescued TRAIL sensitivity in TRAIL-resistant cells, providing a potential new approach to sensitize the cells to TRAIL-induced apoptosis
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