60 research outputs found

    NMR and computational data of two novel antimicrobial peptides

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    AbstractHere we report details on the design and conformational analysis of two novel peptides showing antimicrobial properties, as reported in the research article, “New antimicrobial peptides against foodborne pathogens: from in silico design to experimental evidence” G. Palmieri, M. Balestrieri, Y.T.R. Proroga, L. Falcigno, A. Facchiano, A. Riccio, F. Capuano, R. Marrone, G. Campanile, A. Anastasio (2016) [1]. NMR data, such as chemical shifts in two different solvents as well as aCH protons deviations from random coil values and NOE patterns, are shown together with the statistics of structural calculations. Strategy and particulars of molecular design are presented

    Lack of evidence so far for Noroviruses in food samples collected on cruise ships sailing the Mediterranean waters

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    Closed living spaces like cruise ship settings create an environment in which enteric viruses including Noroviruses (NoVs) can easily spread and outbreaks readily occur. The contribution of sources other than common confirmed vehicles to foodborne illness in cruise ships was investigated. None of the 189 collected samples (including meat, fish, buffet meals, fruits, vegetables etc.) tested by real-time RT-PCR (ISO/TS 15216-2:2013) reveled NoVs genome. Naturally contaminated food items other than shellfish have been demonstrated to be cause of NoVs infection thus, our results could neither indicate nor exclude food items as possible vehicle of infection. Reducing risk factors associated with NoVs outbreaks (contaminated raw ingredients, cross contamination, infected food handlers, inadequate temperature controls, heat treatments) is always strongly recommended

    Toxoplasma gondii in sheep from the Campania region (Italy)

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    A cross-sectional serological survey was conducted in order to evaluate, irrespective of abortion, the Toxoplasma gondii infection in pastured sheep from the Campania region of southern Italy. A geographical information system was used in order to uniformly sample the ovine farms (n = 117) throughout the entire region. Blood and milk samples were collected from 10 adult sheeps (>18 months) on each farm (total number = 1170 sheeps). Serum samples were tested for the presence of IgG antibodies to T. gondii using a commercial indirect fluorescent antibody test. For each farm, the 10 milk samples collected were pooled in order to obtain a single milk sample per farm (total number = 117 milk samples). The 77.8% (91/117) of the farms and the 28.5% (333/11,170) of the sheep resulted positive by serology. In addition, the presence of T. gondii DNA was detected by PCR in 4 milk samples out of the 117 examined (3.4%)
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