1,726,998 research outputs found
Inhibition of XJ-160 virus replication by RNAi
No full textObjective: By construct the model of RNAi-mediated inhibition of XJ-160 virus replication to study RNAi-mediated inhibition of XJ-160 virus replication on aspect of sequence, positional and dose dependent, to further research on effect and mechanism of RNAi on antiviral effects. Methods: Synthesized the XJ-160 virus special short interferencing RNA (siRNA) according to the requiring of siRNA design. siRNA was transfected into BHK-21 cells via liposomes, then infected the BHK-21 cells by XJ-160 virus. Through detecting the titer, protein expression and RNA synthesizing of XJ-160 virus, research on siRNA-mediated inhibition of XJ-160 virus replication. Results: Constructed the model of RNAi-mediated inhibition of XJ-160 virus replication successfully, and discussed the character of it. Conclusion: siRNA can inhibit the replication of XJ-160 virus. It has sequence, positional and dose dependent. And it is carried out by digested the virus RNA.link_to_subscribed_fulltex
XJ: Facilitating XML Processing in Java
No full textThe increased importance of XML as a data representation format has led to several proposals for facilitating the development of applications that operate on XML data. These proposals range from runtime API-based interfaces to XMLbased programming languages. The subject of this paper is XJ, a research language that proposes novel mechanisms for the integration of XML as a first-class construct into Java. The design goals of XJ distinguish it from past work on integrating XML support into programming languages --- specifically, the XJ design adheres to the XML Schema and XPath standards. Moreover, it supports inplace updates of XML data thereby keeping with the imperative nature of Java. We have built a prototype compiler for XJ, and our preliminary experiments demonstrate that the performance of XJ programs can approach that of traditional low-level API-based interfaces, while providing a higher level of abstraction
Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15
No full textIn this study, Scenedesmus obtusus XJ-15 was firstly selected from seven strains microalgae (Chlorophyta, Scenedesmaceae) and then cultivated using a two-stage strategy, which composed of fast cell growth in stage I and followed by lipid induction in stage II in 5-L flasks outdoors. In stage I, the biomass productivity was increased from 139.4 to 212.1 mg L-1 d(-1). In stage II, lipid content was increased from 26.1% to 47.7% by adding NaCl into the culture. This two-stage process was also realized in an 140-L photobioreactor outdoors, with a biomass productivity of 86.5 mg L-1 d(-1) and CO2 fixation rate of 170.0 mg L-1 d(-1) in the first stage, and high lipid content of 42.1% in the second stage. With such a culture strategy, the overall lipid productivity was improved and better biodiesel quality was obtained. These results suggested the photoautotrophic two-stage system was not only feasible but also effective. (C) 2013 Elsevier Ltd. All rights reserved.In this study, Scenedesmus obtusus XJ-15 was firstly selected from seven strains microalgae (Chlorophyta, Scenedesmaceae) and then cultivated using a two-stage strategy, which composed of fast cell growth in stage I and followed by lipid induction in stage II in 5-L flasks outdoors. In stage I, the biomass productivity was increased from 139.4 to 212.1 mg L-1 d(-1). In stage II, lipid content was increased from 26.1% to 47.7% by adding NaCl into the culture. This two-stage process was also realized in an 140-L photobioreactor outdoors, with a biomass productivity of 86.5 mg L-1 d(-1) and CO2 fixation rate of 170.0 mg L-1 d(-1) in the first stage, and high lipid content of 42.1% in the second stage. With such a culture strategy, the overall lipid productivity was improved and better biodiesel quality was obtained. These results suggested the photoautotrophic two-stage system was not only feasible but also effective. (C) 2013 Elsevier Ltd. All rights reserved
ABSTRACT XJ: Integration of XML Processing into Java TM
No full textThe increased importance of XML as a universal data representation format has led to several proposals for enabling the development of applications that operate on XML data. These proposals range from runtime API-based interfaces to XML-based programming languages. The subject of this paper is XJ, a research language that proposes novel mechanisms for the integration of XML as a first-class construct into Java TM. The design goals of XJ distinguish it from past work on integrating XML support into programming languages — specifically, the XJ design adheres to the XML Schema and XPath standards, and supports in-place updates of XML data thereby keeping with the imperative nature of Java. We have also built a prototype compiler for XJ, and our preliminary experimental results demonstrate that the performance of XJ programs can approach that of traditional low level API-based interfaces, while providing a higher level of abstraction
Diagram of the generation of defective XJ-160 replicons.
No full text<p>(A) The pVa-XJ replicon was constructed by inserting the XJ-160 virus genome into the eukaryotic expression vector pVAX1 and replacing the structural gene with the multiple cloning site (MCS) sequence. (B) The constructs containing the reporter gene (pVaXJ-EGFP or pVaXJ-GLuc) were generated by digesting the <i>enhanced green fluorescent protein</i> (<i>EGFP</i>) gene or <i>Gaussia luciferase</i> (<i>GLuc</i>) gene with the restriction enzymes <i>Fse</i>I and <i>Asc</i>I, and ligating them into the MCS of pVa-XJ. (C) The defective XJ-160 replicons (pVaXJ-EGFPΔnsp4 and pVaXJ-GLucΔnsp4) were produced by introducing an 1139-nt deletion mutation in the non-structural protein coding regions of pVaXJ-EGFP and pVaXJ-GLuc using <i>Acl</i>I digestion. The deleted regions of the XJ-160 genome in defective replicons are denoted by dotted lines and the non-structural protein gene deletion is designated by a “Δ”.</p
ABSTRACT XJ: Facilitating XML Processing in Java TM
No full textThe increased importance of XML as a data representation format has led to several proposals for facilitating the development of applications that operate on XML data. These proposals range from runtime API-based interfaces to XMLbased programming languages. The subject of this paper is XJ, a research language that proposes novel mechanisms for the integration of XML as a first-class construct into Java TM. The design goals of XJ distinguish it from past work on integrating XML support into programming languages — specifically, the XJ design adheres to the XML Schema and XPath standards. Moreover, it supports inplace updates of XML data thereby keeping with the imperative nature of Java. We have built a prototype compiler for XJ, and our preliminary experiments demonstrate that the performance of XJ programs can approach that of traditional low-level API-based interfaces, while providing a higher level of abstraction
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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