140,115 research outputs found
Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group
No full textCaged near-IR emitting fluorescent dyes are in high demand in optical microscopy but up to now were unavailable. We discovered that the combination of a carbopyronine dye core and a photosensitive 2-diazo-1-indanone residue leads to masked near-IR emitting fluorescent dyes. Illumination of these caged dyes with either UV or visible light (λ < 420 nm) efficiently generates fluorescent compounds with absorption and emission at 635 nm and 660 nm, respectively. A high-yielding synthetic route with attractive possibilities for further dye design is described in detail. Good photostability, high contrast, and a large fluorescence quantum yield after uncaging are the most important features of the new compounds for non-invasive imaging in high-resolution optical microscopy. For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group.Fil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Wurm, Christian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Sednev, Maksim V.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemani
Typologie der Subjektverkettung : ("Switch reference")
No full textDer Terminus "switch-reference" ist eine Schöpfung von W. Jacobson (1967). Er bezog sich auf ein Phänomen, das zunächst in den putativen Hokan-Sprachen Tonkawa, Washo und Kashaya (Southern Pomo) entdeckt worden war. Es wurde bald als areales Merkmal von Sprachen des Südwestens Nordamerikas erkannt. Es findet sich in allen Great-Basin-Sprachen sowie der westlichen Hälfte des Südwest-Phylums und in Teilen der Phyla Kalifornien, Plateau, Plains und Südost (Jacobson 1983:172). [...] Seit einigen Jahren bringt man diese Erscheinung funktional und terminologisch in Verbindung mit den sog. Medialverben der Papua-Sprachen […]. "Switch-reference" ist die oppositionelle explizite Signalisierung der Identitätsrelation zwischen dem Subjekt des Satzes, an dem die Markierung vorgenommen wird (oder dem sie unmittelbar folgt) und dem Subjekt eines kommenden Satzes (vorzugsweise des nächsten). Sie hat also kriterial eine antizipatorische Komponente. Sie tritt in verbfinalen Sprachen auf und wird deshalb meist durch Enklitika oder Suffixe verkörpert (quasi als Brücke zum folgenden Satz), die den markierten Satz subordinieren. Mit der Subjektidentität (die zwangsläufig auf die eine oder andere Weise mit den Kategorien Person und Numerus interagiert) gehen meist andere Bedeutungen einher, vorzugsweise interpropositionale. Die "switch-reference" hat logischerweise zwei Optionen: Disjunktheit, "different subject" (DS) , und Identität "same subject" (SS). "Oppositionelle Signalisierung" impliziert, daß eine Option gegenüber der anderen keinen grundlegenden Umbau der Satzstruktur erfordert. Davon unberührt bleibt die Tatsache, daß DS-Markierung merkmalhaft er ist. [...] Die Arbeit sollte sich ursprünglich auf Papua- und amerindische Sprachen erstrecken. Obwohl auch letztere ausgiebig untersucht wurden, kam die Darstellung nicht über die Verhältnisse in den Papua-Sprachen hinaus. Angesichts der Sprachenvielfalt scheint mir der Begriff Typologie im Titel noch gerechtfertigt. Ebenfalls keine Berücksichtigung fand die SV der südamerikanischen Sprachen, wofür die Untersuchungsgrundlage aber ohnehin dürftig gewesen wäre. Verwandte Phänomene in australischen, kaukasischen und afrikanischen Sprachen werden im Kapitel 8 lediglich gestreift
Odorant Binding Proteins of the Red Imported Fire Ant, Solenopsis invicta: An Example of the Problems Facing the Analysis of Widely Divergent Proteins
No full textPMCID: PMC3031547This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose
Jorge F. Lima González Bonorino y Hernán Carlos Lux-Wurm, Colección de Documentos sobre los Conquistadores y Pobladores del Río de la Plata, Buenos Aires, Argentina, Revista del Instituto Histórico Municipal de San Isidro, 2001, 282 pp.
No full textLa presente Colección de Documentos de los genealogistas argentinos Lima González Bonorino y Lux-Wurm, reúne una serie de antecedentes sobre los conquistadores y pobladores del Río de la Plata y otras zonas de América del Sur que, hasta el momento, se encontraban dispersos y de difícil reunión. De hecho, cada vez que se debía indagar sobre los integrantes de la Conquista, civiles y religiosos, era necesario concurrir a distintos repositorios y bibliotecas, con el objeto de ubicar el material ..
Spatially-localized time dependent solutions including turbulence and their interactions in 2D Kolmogorov flow
No full textIn 2D Kolmogorov flow in small aspect ratio domains, spatially-localized solutions such as kink, traveling or time-dependent kink-antikink pars coexist. However, the conservation of the flow rate in the y direction strongly restrict combination of localized solutions and their positioning. We find that by adding a homogeneous flow U y their positioning is controlled and each of localized solutions including a spatially-localized chaos is isolated. Numerical results suggest that these isolated solutions can be elements constructing a whole flow
Characteristics of overlap region in high-Reynolds number turbulent channel flow
No full textDirect numerical simulation of the fully developed turbulent channel flows have been carried out at the Reynolds number based on the friction velocity and the channel half width, 2000, 4000 and 8000. A hybrid 10th order accurate finite difference scheme in the stream and spanwise directions, and a second-order scheme in the wall-normal direction is adapted as the spatial discretization method. We observed the plateau profiles in the indicator function corresponded to the von Karman constant. Furthermore, second peak of streamwise pre-multiplied spectra were appeared in the same wall normal height, 300 < y+ < 600, in case of Re = 4000. Nevertheless, the effects of the lager than the channel half height scale on the streamwise turbulent intensity are fixed contributions without dependence on Reynolds number. These results suggested that the new streamwise vortexes are formed between buffer layer and outer layer with increasing of Reynolds number
La 'circunstancia' de 'Herederos y Pretendientes
No full textIn June 2010, the Ortega y Gasset Foundation hosted a Conference about the “Spanish Philosophical Transition” in order to debate the book of Francisco Vázquez, La filosofía española. Herederos y Pretendientes. Una lectura sociológica (1963-1990), recently published. This paper is the author’s response to criticism raised in the Conference and to published reviews received by this book. First, the author summarized the argument of Herederos y pretendientes. Secondly he responds and takes into account the most important objections against the book’s hypothesis and methodology. Finally the author evaluates the favorable judgments received by the book and suggests the limits of the historian’s task.Fundación Ortega y Gasset-Marañó
Coincidencias y divergencias: arquitectura e instalaciones artísticas
No full textA comparative study is presented whose objective is to highlight the similarities and differences that exist between architecture and artistic installations. Four case studies are analyzed, and in each one of them a couple of works from both disciplines are contrasted, which were selected considering the common use of certain visual resources: One-Sqm-House, by Van Bo Le-Mentzel and Measurement Rooms, by Mel Bochner; Jade Eco Park, by Philippe Rahm, Inert Gas Series and Ultrasonic Wave Piece, by Robert Barry; Roulotte, by Xavier Arenòs, Domēnec, Martí Peran and Just about Virtues and Vices in General, by Erwin Wurm; the Hans-Walter Müller Refugee Center and Michael Rakowitz’s paraSITE homeless shelter. This analysis allows us to glimpse the great diversity of aspects that bring together or separate these two worlds linked by their interest in space.Se presenta un estudio comparativo cuyo objetivo es destacar las similitudes y diferencias que existen entre la arquitectura y las instalaciones artísticas. Se analizan cuatro casos de estudio, y en cada uno de ellos se contrasta un par de obras procedentes de ambas disciplinas, mismas que fueron seleccionadas considerando el uso común de ciertos recursos visuales: One-Sqm-House, de Van Bo Le-Mentzel y Measurement Rooms, de Mel Bochner; Jade Eco Park, de Philippe Rahm, Inert Gas Series y Ultrasonic Wave Piece, de Robert Barry; Roulotte, de Xavier Arenòs, Domēnec, Martí Peran y Just about Virtues and Vices in General, de Erwin Wurm; el Centro de refugiados de Hans-Walter Müller y paraSITE homeless shelter, de Michael Rakowitz. Este análisis permite vislumbrar la gran diversidad de aspectos que aproximan o separan a estos dos mundos vinculados por su interés en el espacio.
 
The Protein Precursors of Peptides That Affect the Mechanics of Connective Tissue and/or Muscle in the Echinoderm Apostichopus japonicus
No full textPMCID: PMC3432112This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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