196,799 research outputs found
Glycoproteomics and Glycomics: Method Development and Clinical Applications
No full textWuhrer, M. [Promotor]Kolarich, D. [Copromotor
Methods for the glycosylation analysis of therapeutic antibodies
No full textWuhrer, M. [Promotor]Engler, N. [Copromotor]Weigand, S. [Copromotor
Absolute quantitation of disease protein biomarkers in a single LC-MS acquisition using apolipoprotein F as an example
No full textLC-MS and immunoassay can detect protein biomarkers. Immunoassays are more commonly used but can potentially be outperformed by LC-MS. These techniques have limitations including the necessity to generate separate calibration curves for each biomarker. We present a rapid mass spectrometry-based assay utilising a universal calibration curve. For the first time we analyse clinical samples using the HeavyPeptide IGNIS kit which establishes a 6-point calibration curve and determines the biomarker concentration in a single LC-MS acquisition. IGNIS was tested using apolipoprotein F (APO-F), a potential biomarker for non-alcoholic fatty liver disease (NAFLD). Human serum and IGNIS prime peptides were digested and the IGNIS assay was used to quantify APO-F in clinical samples. Digestion of IGNIS prime peptides was optimised using trypsin and SMART Digest™. IGNIS was 9 times faster than the conventional LC-MS method for determining the concentration of APO-F in serum. APO-F decreased across NAFLD stages. Inter/intra-day variation and stability post sample preparation for one of the peptides was ≤13% coefficient of variation (CV). SMART Digest™ enabled complete digestion in 30 minutes compared to 24 hours using in-solution trypsin digestion. We have optimised the IGNIS kit to quantify APO-F as a NAFLD biomarker in serum using a single LC-MS acquisition
New Perspectives in Shape Analysis (Dagstuhl Seminar 14072)
No full textOver the last decade, it has become increasingly affordable to digitize 2D and 3D shape information using multiple modalities, such as (video) cameras, image-based reconstruction systems, laser-range scanners, or depth cameras. If these dense models can be processed and described in an efficient and informative way, they can be used in applications, such as ergonomic design, virtual shopping, scientific and medical visualization, realistic simulation, photo-realistic rendering, the design of natural user interfaces, and semantic scene understanding.
Traditionally, the notion of shape has been studied either by analyzing projections of shapes in images or by analyzing a sparse set of marker positions on 3D shapes. Typical tasks in 2D shape analysis include segmenting objects in images and tracking objects across a sequence of images, and typical tasks in 3D shape analysis include reconstructing the three-dimensional object depth from input images and identifying corresponding points on different 3D models. The analysis and processing of shape data becomes especially challenging because of the increasing amount of data captured by sensors used to acquire shapes, and because modern applications such as natural user interfaces require real-time processing of the input shapes.
Meeting these challenges requires models of shape analysis that are compact and informative, thereby allowing the development of algorithms that can process large datasets efficiently. To achieve these goals, interdisciplinary approaches are needed that use concepts from a variety of research areas, including numerical computing, differential geometry, deformable shape modeling, sparse data representation, and machine learning. On the algorithmic side, many shape analysis tasks are modeled using partial differential equations, which can be solved using tools from the field of numerical computing. The fields of differential geometry and deformable shape modeling have recently begun to influence shape analysis methods. Furthermore, tools from the field of sparse representations, which aim to describe input data using a compressible representation with respect to a set of carefully selected basis elements, have the potential to significantly reduce the amount of data that needs to be processed in shape analysis tasks. The related field of machine learning offers similar potential.
This seminar brought together 28 researchers from North America and Europe engaged in recent and upcoming developments in shape analysis who view these challenges from different perspectives and who together discussed the pressing open problems and novel solutions to them
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States" By M. Carey.
No full text"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States: containing bried sketches of the moral and political character of those states.
By M. Carey, member of the American philosophical, and of the American Antiquarian Society, and author of The Olive Branch, Cindiciae Hibernicae, essays on banking, on political economy, and on internal improvement.
To which are now added the English editor's comments on the subject; together with Important Advice to Emigrants, and Cautions Against Impositions Practiced in the Outports
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Dr. Glendon Swarthout
No full textHosted by Roger M. Busfield, MSU Assistant Professor of Speech and Theater, Meet the Author is designed to introduce a general audience to a contemporary author and their work through in-depth interviews. This episode features a conversation between Dr. Glendon Swarthout, prolific author and English professor at MSU, and assistant professors Sam S. Baskett and Theodore B. Strandness
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