1,721,367 research outputs found

    Nécrologie : Wu Shang Shih (1904-1947)

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    Allix André. Nécrologie : Wu Shang Shih (1904-1947). In: Revue de géographie jointe au Bulletin de la Société de géographie de Lyon et de la région lyonnaise, vol. 24, n°2, 1949. pp. 170-172

    Le loess durci du Mont-d'Or lyonnais

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    Wu Shang Shih. Le loess durci du Mont-d'Or lyonnais. In: Les Études rhodaniennes, vol. 14, n°4, 1938. pp. 235-242

    Un « morvan » en Chine : Nan Yo, la montagne sacrée du Sud, étude morphologique

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    Wu Shang Shih. Un « morvan » en Chine : Nan Yo, la montagne sacrée du Sud, étude morphologique. In: Revue de géographie jointe au Bulletin de la Société de géographie de Lyon et de la région lyonnaise, vol. 24, n°2, 1949. pp. 109-116

    Wu shang bi yao 无 上 秘 要.

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    Sha zhou 沙 州. Shen quan guan 神 泉 觀. Ma Bao yi 馬 抱 一. Ma Chu you 馬 處 幽. Dun huang xian 燉 煌 縣. Wu shang bi yao 无 上 袐 要. ColophonsNumérisation effectuée à partir d'un document original.J. 33, [qing chuan shou fa pin 輕 傳 受 罰 品,] déb. manque. Raresvariantes par rapport à Dao 1130, j. 33, fasc. 772, 子 一, ff. 1 b 10-6 a. A gauche du titre final,colophon inscrit en petits car. dans la partie inf. de la f. 5 : « Texterespectueusement copié, en acte d'offrande, le 8e jour du 2e mois de la 6eannée kai yuan 開 元 (718), la copie étant due à MaChu you 馬 處 幽 et à son neveu Ma Bao yi 馬 抱 一, daoshi 道 士 du Shen quan guan 神 泉 觀 de la sous-préfecture de Dun huang 燉 煌 縣,préfecture de Sha 沙 州 en faveur de leurs ancêtres décédés jusqu'à la 7egénération, de leurs pères et mères et de tous les êtres vivants... » (3 col.,49 car.). Même colophon que celui des Pelliot chinois 2602recto, Pelliot chinois 2861 recto 1, Pelliotchinois 3141 recto, S. 80 recto et Pékin 珍 20. Repr. partielle(2 dernières col. du texte et colophon) in TKRS , vol. 2, pl. 40. Cf. TKDM , p. 92 ;CLT , p. 366. Même main? que Pelliot chinois 2602 recto, Pelliot chinois2861 recto 1 et Pelliot chinois 3141 recto. Belle écr. call., encre foncée. 84 col. en tout. 28 col. par f., 16 ou 17car. par col. Marges sup. 2,7 à 2,9 cm, inf. 1,9 à 2,7 cm.Réglure

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Conformational Changes of Chloroplast ATP Synthase γ Subunit Studied by Single Molecule Detection Methods

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    隨著生化科技時代的來臨,研究生物體的結構以及運動方式,而解開許多生物遺傳及特性的奧秘,成為生物化學的研究主流。本篇論文利用單分子偵測技 術,探討如何解開生物體的運動模式。單分子偵測由於可以讓生物分子處於活性 狀態時,同步觀察其動態行為,近幾年來,成為探索許多生物機制的利器。 葉綠體 ATP 合成脢( Chloroplast ATP Synthase,CF0F1 )之γ 亞基( γ subunit )具有一個特殊的區域--- dithiol containing domain,藉由其中的 disulfide bond(Cys199,Cys205)會因環境不同而呈現氧化或還原作用來調控 ATPase 的狀態。根據模擬結果發現,γ亞基的構型會因為 ATPase 的狀態不同而改變;當 Cys199,Cys205 氧化成 disulfide bond 時,γ亞基變成一個較為緊密的構型 (close state),造成γ亞基不易旋轉使得 ATPase 活性下降(latent)。當Cys199,Cys205為還原態,因為 dithiol domain 向上移動呈 open state,使γ亞基可以自由旋轉而提升活性( active )。γ亞基共有四個 cystienes(Cys89,Cys199,Cys205,Cys322),所以我們必須從菠菜中萃取出氧化態 ATPase,保留Cys89和Cys322作為標記(labeling)染料的位置,利用 Hydroxyapatite Columns純化出 γ亞基,先由圓二色光譜儀 (CD)確認為自然態(native state),再利用 ESI-MS 及 MALDI-TOF MS 確認染料標記的數目及標記的位置,最後利用單分子螢光偵測技術觀察γ亞基在native state以及 unfoling 之間的構型變化。本篇論文從如何由菠菜中萃取出 ATP 合成脢談起,再由其中純化出 γ 亞基,再談到標記染料並利用質譜儀確認標記染料的個數,最後再利用單分子螢光偵測技術觀察蛋白質構型變化。The chloroplast F0F1-ATP synthase - ATPase is a tiny rotary motor responsible for coupling ATP synthesis and hydrolysis to the light-driven electrochemical proton gradient. Reversible oxidation/reduction of a dithiol, located within a special regulatory domain of the subunit of the chloroplast F1 enzyme, switches the enzyme between an inactive and an active state. This regulatory mechanism is unique to the ATP synthase of higher plants. Molecular dynamic studies predicted that the dithiol domain exists in a range of conformations between two extreme states;the fully open state, with the dithiol reduced ,and a fully closed state with dithiol oxidized to form a disulfide bridge. The open conformation is predicted to represent the activated state and the closed conformation is inactivated state. We isolated CF0F1 from spinach leaves. The γ subunit of spinach CF1 contains four cysteinyl sulfydryls, two of which (Cys199 and Cys205) are linked together to form the disulfide bridge. We isolated γ subunit from spinach ATPase by Hydroxyapatite Columns, and confirmed the native state of γ subunit by CD spectroscopy. We labeled TMR and Cy5 as donor and acceptor on γ subunit, confirm the numbers of dyes on γ subunit by ESI-MS, and confirmed the positions of dye on γ subunit by MALDI-TOF MS. Finally, we observed the change of conformations of γ subunit between folding and unfolding state by single molecular detection methods.口試委員審定書………………………………………………………..i 誌謝…………………………………………………………………..ii 中文摘要…………………………………………………………….iii 英文摘要……………………………………………………………….iv 圖目錄………………………………………………………………..vi 表目錄……………………………………………………………….viii 第一章 序論 1 1.1 動機…………………………………………………………… …1 1.2 葉綠體 ATP 合成脢的結構………………………………… ….1 1.3 γ和ε subunits 在葉綠體 ATPase 中所扮演的角色…………5 1.4 單分子偵測方式……………………………………………… ..10 第二章 實驗方法………………………………………………… .15 2. 1 萃取氧化態 CF0F1………………………………………15 2.1.1 從菠菜葉上分離出葉綠體……………………………..15 2.1.2 萃取類囊體膜蛋白………………………………………15 2.1.3 以硫酸銨沉澱法得到初分葉綠素………………………16 2.1.4 利用蔗糖梯度離心法在純化葉綠素……………………16 2.2 蛋白質濃度的測定…………………………………………...17 2.3 利用膠體電泳確認 CF0F1亞基的完整…………………………17 2.3.1 樣品配置…………………………………………...18 2.3.2 電泳分離…………………………………………...18 2.4 鑑定CF0F1為氧化態……………………………………………20 2.5 利用 Hydroxyapatite Columns純化 γ subunit………………22 2.5.1 蛋白質的吸附………………………………………...22 2.5.2 蛋白質的沖堤機制…………………………………...23 2.5.3 從 CF0F1 中純化 γ subunit ………………………..23 2.5.4 蛋白質溶液的透析…………………………………...24 2.5.5 蛋白質溶液的濃縮…………………………………...26 2.6 確認蛋白質為自然態……………………………………….27 2.6.1 製備蛋白質樣品……………………………………...29 2.7 Dye Labeling…………………………………………………..30 2.8共聚焦(Con-focal)顯微術…………………………………...34 第三章 結果與討論………………………………………………..45 3.1 氧化態CF0F1的萃取與純度鑑定………………………..36 3.2 氧化態CF0F1的鑑定…………………………………...37 3.3 以 HA 純化 γ subunit…………………………….37 3.4 熱變性效應…………………………………………....53 3.5 Dye Labeling…………………………………….....59 3.6 單分子偵測及數據處理方式…………………………...61 第四章 結論與未來展望…………………………………………..66 參考文

    Higher-Order Couplings in the Preheating Theory after Inflation

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    The energy scale during preheating after inflation can be very high, namely, about 1013GeV. In this thesis, we investigate the preheating theory by introducing higher- order coupling terms to extend the preheating theory after inflation to high energy scale and try to verify the robustness of the preheating theory. We find that the preheating theory is robust only for small coupling constants. Higher-order coupling terms might enhance or suppress the parametric resonance, and lead to the preheating more or less efficient.Chapter 1 Introduction 7 Chapter 2 FRW cosmology and inflation 9 2.1 A brief review of FRW big bang standard cosmology 2.2 Inflation Chapter 3 Reheating 13 3.1 Elementary theory of reheating – perturbative decay 3.2 Preheating 3.2.1 Parametric resonance 3.2.2 Stochastic resonance Chapter 4 Higher-order coupling corrections 29 4.1 Motivation 4.2 Analytical calculation 4.3 Numerical results Chapter 5 Summary and prospects 45 Appendix 49 Appendix A Quantum field theory in a time- dependent background Bibliography 5
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