75 research outputs found

    Additional_Table_S1_5 – Supplemental material for Integrative Analysis of Genome-Wide Association Studies and DNA Methylation Profile Identified Genetic Control Genes of DNA Methylation for Kashin-Beck Disease

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    Supplemental material, Additional_Table_S1_5 for Integrative Analysis of Genome-Wide Association Studies and DNA Methylation Profile Identified Genetic Control Genes of DNA Methylation for Kashin-Beck Disease by Ping Li, Cuiyan Wu, Xiong Guo, Yan Wen, Li Liu, Xiao Liang, Yanan Du, Lu Zhang, Mei Ma, Shiqiang Cheng, Bolun Cheng, Sen Wang and Feng Zhang in CARTILAGE</p

    Additional_Table_S2_GO_Enrichment_P_value_1 – Supplemental material for Integrative Analysis of Genome-Wide Association Studies and DNA Methylation Profile Identified Genetic Control Genes of DNA Methylation for Kashin-Beck Disease

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    Supplemental material, Additional_Table_S2_GO_Enrichment_P_value_1 for Integrative Analysis of Genome-Wide Association Studies and DNA Methylation Profile Identified Genetic Control Genes of DNA Methylation for Kashin-Beck Disease by Ping Li, Cuiyan Wu, Xiong Guo, Yan Wen, Li Liu, Xiao Liang, Yanan Du, Lu Zhang, Mei Ma, Shiqiang Cheng, Bolun Cheng, Sen Wang and Feng Zhang in CARTILAGE</p

    Microbial mechanisms of organic matter mineralization induced by straw in biochar-amended paddy soil

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    Abstract Combined straw and straw-derived biochar input is commonly applied by farmland management in low-fertility soils. Although straw return increases soil organic matter (SOM) contents, it also primes SOM mineralization. The mechanisms by which active microorganisms mineralize SOM and the underlying factors remain unclear for such soils. To address these issues, paddy soil was amended with 13 C-labeled straw, with and without biochar (BC) or ferrihydrite (Fh), and incubated for 70 days under flooded conditions. Compound-specific 13 C analysis of phospholipid fatty acids ( 13 C-PLFAs) allowed us to identify active microbial communities utilizing the 13 C-labeled straw and specific groups involved in SOM mineralization. Cumulative SOM mineralization increased by 61% and 27% in soils amended with Straw + BC and Straw + Fh + BC, respectively, compared to that with straw only. The total PLFA content was independent of the straw and biochar input. However, 13 C-PLFAs contents increased by 35–82% after biochar addition, reflecting accelerated microbial turnover. Compared to that in soils without biochar addition, those with biochar had an altered microbial community composition-increased amounts of 13 C-labeled gram-positive bacteria ( 13 C-Gram +) and fungi, which were the main active microorganisms mineralizing SOM. Microbial reproduction and growth were susceptible to nutrient availability. 13 C-Gram + and 13 C-fungi increased with Olsen P but decreased with dissolved organic carbon and NO3{\text{NO}}_{3}^{ - } NO 3 - contents. In conclusion, biochar acts as an electron shuttle, stimulates iron reduction, and releases organic carbon from soil minerals, which in turn increases SOM mineralization. Gram + and fungi were involved in straw decomposition in response to biochar application and responsible for SOM mineralization. Graphical AbstractNational Natural Science Foundation of China http://dx.doi.org/10.13039/501100001809Ningbo Municipal Bureau of Science and Technology http://dx.doi.org/10.13039/501100007928the “Pioneer” and “Leading Goose” R&D Program of Zhejian

    LARC 5665 Design Studio 5: the Urban Landscape, 2010 Uptown Cole Park, Professor Taner Ozdil.

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    Selected student work from the 2010 landscape architecture course LARC 5665 Design Studio 5: the Urban Landscape, Professor Taner Ozdil. Student work includes Cole Park and Uptown Village, Dallas, Texas; Cameron Holmes, Alexandra Leister, Grace Herman, Yunhui Zhou, Susan Alford, Sarah Kuehn, Chia-Yin Wu, Yao Lin, Cuiyan Mei, and Rhonda Fields

    LARC 5665 Design Studio 5: the Urban Landscape, 2010 Junius Heights, Professor Taner Ozdil.

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    Selected student work from the 2010 landscape architecture course LARC 5665 Design Studio 5: the Urban Landscape, Professor Taner Ozdil. Student work includes Junius Heights, Dallas, Texas; Cuiyan Mei, Yao Lin, Chia-Yin Wu, Yunhui Zhou, Alexandra Leister, and Rhonda Fields

    Physicochemically protected organic carbon release is the rate-limiting step of rhizosphere priming in paddy soils

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    http://dx.doi.org/10.13039/501100007928 Ningbo Science and Technology Bureauhttp://dx.doi.org/10.13039/501100001809 National Natural Science Foundation of Chin

    Characterization of <i>Alternaria</i> Species Associated with Black Spot of Strawberry in Dandong, China

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    Dandong has become the largest strawberry production and export base in China. Strawberry black spot disease is widespread and causes significant economic losses to strawberry growers in both the growing and harvest seasons. Until now, no study has reported the presence of the Alternaria species, the pathogen of strawberry black spot disease, in Dandong, Liaoning province, China. In 2020–2022, 108 isolates were obtained from strawberry leaves with typical symptoms of strawberry black spot disease from 56 major professional growing operations. Combined with morphological and molecular characteristics, the majority of isolates were identified as A. tenuissima (78 isolates, 72.2%), which had established total supremacy, followed by A. alternata (30 isolates, 27.8%). The pathogenicity results show that A. tenuissima and A. alternata are the two main pathogenic factors of strawberry black spot disease, the disease indexes of which were designated as 49.6–100.0% and 20.4–59.5%. To our knowledge, this paper is the first to identify A. tenuissima and A. alternata as causing black spot disease in strawberries in Dandong, China

    Cell cycle-related lncRNAs and mRNAs in osteoarthritis chondrocytes in a Northwest Chinese Han Population

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    Background: A group of differentially expressed long non-coding RNAs (lncRNAs) have been shown to play key roles in osteoarthritis (OA), although they represented only a small proportion of lncRNAs that may be biologically and physiologically relevant. Since our knowledge of regulatory functions of non-coding RNAs is still limited, it is important to gain better understanding of their relation to the pathogenesis of OA. Methods: We performed mRNA and lncRNA microarray analysis to detect differentially expressed RNAs in chondrocytes from three OA patients compared with four healthy controls. Then, enrichment analysis of the differentially expressed mRNAs was carried out to define disease molecular networks, pathways and gene ontology (GO) function. Furthermore, target gene prediction based on the co-expression network was performed to reveal the potential relationships between lncRNAs and mRNAs, contributing an exploration of a role of lncRNAs in OA mechanism. Quantitative RT-PCR analyses were used to demonstrate the reliability of the experimental results. Findings: Altogether 990 lncRNAs (666 up-regulated and 324 down-regulated) and 546 mRNAs (419 up-regulated and 127 down-regulated) were differentially expressed in OA samples compared with the normal ones. The enrichment analysis revealed a set of genes involved in cell cycle. In total, 854 pairs of mRNA and lncRNA were highly linked, and further target prediction appointed 12 genes specifically for their corresponding lncRNAs. The lncRNAs lncRNA-CTD-2184D3.4, ENST00000564198.1, and ENST00000520562.1 were predicted to regulate SPC24, GALM, and ZNF345 mRNA expressions in OA. Interpretation: This study uncovered several novel genes potentially important in pathogenesis of OA, and forecast the potential function of lnc-CTD-2184D3.4, especially for the cell cycle in the chondrocytes. These findings may promote additional aspects in studies of OA
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