2,492 research outputs found
Conversation with Cam Winton, Candidate for Minneapolis Mayor
No full textCam Winton is the only Independent candidate in the Minneapolis Mayoral race. How does his experience and vision for the city differ from those of his DFL counterparts? Come hear some of his ideas in a conversation moderated by Professor Larry Jacobs.Center for the Study of Politics and Governance, Humphrey School of Public Affairs, UMNWinton, Cam; Jacobs, Lawrence. (2013). Conversation with Cam Winton, Candidate for Minneapolis Mayor. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/194681
The Effect of Using Computer Technology Tools to Enhance the Teaching-Learning Process in CAD-CAM-CNC Module in Mechanical Engineering Subject Area
No full textThis study presents the evaluation of Computer Assisted Learning (CAL) package included in the teaching and learning methodology of computer aided design- computer aided design - computer numerical control (CAD-CAM-CNC) module.
Three groups of students with similar pre-abilities were exposed to three different teaching learning methodologies.The effectiveness of these three methods was determined by questionnaires completed by the students and collected by first author. Their answers were analysed quantitatively and qualitatively. The various categories used in the questionnaire was student’s attitudes towards learning CAD-CAM-CNC subjects, students’ opinions about their lecturers approaches to teaching process, students’ opinions and views about various aspects the CAD-CAM-CNC. The study concludes that the students taught with a combination of CAL package and traditional methods were more effective, efficient and satisfied with their learning experiences. So the proposed hybrid learning method (CAI plus traditional teaching method) is most suited for CAD-CAM-CNC teaching.
Computer Technology; Computer-Assisted Instruction (CAI); Computer Assisted Learning (CAL); Computer Aided Design (CAD); Computer Aided Manufacturing (CAM); Computer Numerical Control (CNC)
Nuclear translocation and signalling of L1-CAM in human carcinoma cells requires ADAM10 and presenilin/gamma-secretase activity
No full textL1-CAM (L1 cell-adhesion molecule), or more simply L1, plays an important role in the progression of human carcinoma. Overexpression promotes tumour-cell invasion and motility, growth in nude mice and tumour metastasis. It is feasible that L1-dependent signalling contributes to these effects. However, little is known about its mechanism in tumour cells. We reported previously that L1 is cleaved by ADAM (a disintegrin and metalloprotease) and that the cytoplasmic part is essential for L1 function. Here we analysed more closely the role of proteolytic cleavage in L1-mediated nuclear signalling. Using OVMz carcinoma cells and L1-transfected cells as a model, we found that ADAM10-mediated cleavage of L1 proceeds in lipid raft and non-raft domains. The cleavage product, L1-32, is further processed by PS (presenilin)/gamma-secretase to release L1-ICD, an L1 intracellular domain of 28 kDa. Overexpression of dominantnegative PS1 or use of a specific gamma-secretase inhibitor leads to an accumulation of L1-32. Fluorescence and biochemical analysis revealed a nuclear localization for L1-ICD. Moreover, inhibition of ADAM10 and/or gamma-secretase blocks nuclear translocation of L1-ICD and L1-dependent gene regulation. Overexpression of recombinant L1-ICD mediates gene regulation in a similar manner to full-length L1. Our results establish for the first time that regulated proteolytic processing by ADAM10 and PS/gamma-secretase is essential for the nuclear signalling of L1 in human carcinoma cell lines. Key words: a disintegrin and metalloprotease 10 (ADAM10), L1 cell-adhesion molecule (L1-CAM), nuclear translocation, presenilin (PS)/gamma-secretase activity, raft, signalling
Design of the CAM model and authoring tool
No full textStudents benefit from personalised attention; however, often teachers are unable to provide this. An Adaptive Hypermedia (AH) system can offer a richer learning experience in an educational environment, by giving personalised attention to students. On-line courses are becoming increasingly popular by means of Learning Management Systems (LSM). The aim of the GRAPPLE project is to integrate an AH with major LMS, to provide an environment that delivers personalised courses in a LMS interface. However, designing an AH is a much more complex and time-consuming task, than creating a course in a LMS. Several models and systems were developed previously, but the (re)-usability by educational authors of the adaptation remains limited. To simplify adaptive behaviour authoring for an educational author, a visual environment was selected as being most intuitive. This paper describes a reference model for authoring in a visual way and introduces an authoring tool based upon this model
Understanding the Development of Cam-Type Deformity by FE Analysis of the Immature Proximal Femur
No full textCam impingement appears to be an important factor in the development of early osteoarthritis (OA) of the hip. A Cam is generally diagnosed in young and active adults, already at the age of thirteen. Therefore, it has been suggested that excessive femoral loading during skeletal development might trigger the abnormal morphology. The goal of this research is to understand how the deformity develops. A finite element analysis is conducted to predict the stress distribution in the immature proximal femur under different loading conditions. Furthermore, the influence of the orientation of the epiphyseal plate is studied, as a Cam-type deformity is highly associated with an abnormal epiphyseal extension on the anterosuperior region. Previous studies suggested that octahedral shear stress promotes growth, while hydrostatic compressive stress inhibits growth. These mechanobiological principals are implemented to predict the bone growth rate. The position and the shape of the growth plate, as well as the loading direction highly influence the stress distribution in the proximal femur and growth plate. A medial directed load causes high compression stress in the growth cartilage in the lateral region. Endochondral growth is inhibited, which could result in a deflection of the growth plate, observed in Cam patients as epiphyseal extension. High regional stresses and osteogenic index appear in the epiphysis at the anterosuperior region when a convex growth plate is modeled. These results would support the theory that a Cam-type deformity might not be a result of endochondral growth, but the higher stresses demand local remodeling and apposition. Incorporating dynamic loading and tissue adaptation is needed to further understand how mechanical stimuli effects the epiphyseal growth and to predict morphological changes over time. Once one understands the etiological factors of cam impingement, the disorder can be treated at an early stage and the development into early osteoarthritis might be prevented.BMEBioMechanical EngineeringMechanical, Maritime and Materials Engineerin
Wound-induced rgs-CaM gets ready for counterresponse to an early stage of viral infection
No full textPlants and animals can recognize the invasion of pathogens through their perception of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs). Plant PRRs identified have been exclusively receptor-like kinases/proteins (RLK/Ps), and no RLK/P that can detect viruses has been identified to date. RNA silencing (RNA interference, RNAi) is regarded as an antiviral basal immunity because the majority of plant viruses has RNA as their genomes and encode RNA silencing suppressor (RSS) proteins to counterattack antiviral RNAi. Many RSSs were reported to bind to double-stranded RNAs (dsRNAs), which are regarded as viral PAMPs. We have recently identified a tobacco calmodulin (CaM)-like protein, rgs-CaM, as a PRR that binds to diverse viral RSSs through its affinity for the dsRNA-binding domains. Because rgs-CaM seems to target RSSs for autophagic degradation with self-sacrifice, the expression level of rgs-CaM is important for antiviral activity. Here, we found that the rgs-CaM expression was induced immediately (within 1 h) after wounding at a wound site on tobacco leaves. Since the invasion of plant viruses is usually associated with wounding, and several hours are required for viruses to replicate to a detectable level in invaded cells, the wound-induced expression of rgs-CaM seems to be linked to its antiviral function, which should be ready before the virus establishes infection. CaMs and CaM-like proteins usually transduce calcium signals through their binding to endogenous targets. Therefore, rgs-CaM is a unique CaM-like protein in terms of binding to exogenous targets and functioning as an antiviral PRR
Underwater CAM photosynthesis elucidated by Isoetes genome
No full textTo conserve water in arid environments, numerous plant lineages have independently evolved Crassulacean Acid Metabolism (CAM). Interestingly, Isoetes, an aquatic lycophyte, can also perform CAM as an adaptation to low CO2 availability underwater. However, little is known about the evolution of CAM in aquatic plants and the lack of genomic data has hindered comparison between aquatic and terrestrial CAM. Here, we investigate underwater CAM in Isoetes taiwanensis by generating a high-quality genome assembly and RNA-seq time course. Despite broad similarities between CAM in Isoetes and terrestrial angiosperms, we identify several key differences. Notably, Isoetes may have recruited the lesser-known ‘bacterial-type’ PEPC, along with the ‘plant-type’ exclusively used in other CAM and C4 plants for carboxylation of PEP. Furthermore, we find that circadian control of key CAM pathway genes has diverged considerably in Isoetes relative to flowering plants. This suggests the existence of more evolutionary paths to CAM than previously recognized. © 2021, The Author(s).Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Cam design and numeric control machining package for Hewlett-Packard Singapore (Pte) Ltd
No full textThis thesis presents a continuation of a previous work that was undertaken to develop a prototype software that integrates the processes of cam design and cam manufacturing for Industrial Automation and Robotics Design use. It starts by presenting some difficulty faced by the author who was unable to find suitable easy-to-use or friendly software for the cam analysis purpose so that the analyse data could help in the design of a cam profile followed by its manufacturing. The software whereby the author has embarked on especially in the area of cam manufacturing was to assist the author's internal support or in-house build industrial automation and robotics projects dealing with high speed cam driven for components pick & place machines.Master of Science (Computer Integrated Manufacturing
rgs-CaM Detects and Counteracts Viral RNA Silencing Suppressors in Plant Immune Priming
No full textPrimary infection of a plant with a pathogen that causes high accumulation of salicylic acid in the plant typically via a hypersensitive response confers enhanced resistance against secondary infection with a broad spectrum of pathogens, including viruses. This phenomenon is called systemic acquired resistance (SAR), which is a plant priming for adaption to repeated biotic stress. However, the molecular mechanisms of SAR-mediated enhanced inhibition, especially of virus infection, remain unclear. Here, we show that SAR against cucumber mosaic virus (CMV) in tobacco plants (Nicotiana tabacum) involves a calmodulin-like protein, rgs-CaM. We previously reported the antiviral function of rgs-CaM, which binds to and directs degradation of viral RNA silencing suppressors (RSSs), including CMV 2b, via autophagy. We found that rgs-CaM-mediated immunity is ineffective against CMV infection in normally growing tobacco plants but is activated as a result of SAR induction via salicylic acid signaling. We then analyzed the effect of overexpression of rgs-CaM on salicylic acid signaling. Overexpressed and ectopically expressed rgs-CaM induced defense reactions, including cell death, generation of reactive oxygen species, and salicylic acid signaling. Further analysis using a combination of the salicylic acid analogue benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) and the Ca2+ ionophore A23187 revealed that rgs-CaM functions as an immune receptor that induces salicylic acid signaling by simultaneously perceiving both viral RSS and Ca2+ influx as infection cues, implying its autoactivation. Thus, secondary infection of SAR-induced tobacco plants with CMV seems to be effectively inhibited through 2b recognition and degradation by rgs-CaM, leading to reinforcement of antiviral RNA silencing and other salicylic acid-mediated antiviral responses. IMPORTANCE Even without an acquired immune system like that in vertebrates, plants show enhanced whole-plant resistance against secondary infection with pathogens; this so-called systemic acquired resistance (SAR) has been known for more than half a century and continues to be extensively studied. SAR-induced plants strongly and rapidly express a number of antibiotics and pathogenesis-related proteins targeted against secondary infection, which can account for enhanced resistance against bacterial and fungal pathogens but are not thought to control viral infection. This study showed that enhanced resistance against cucumber mosaic virus is caused by a tobacco calmodulin-like protein, rgs-CaM, which detects and counteracts the major viral virulence factor (RNA silencing suppressor) after SAR induction. rgs-CaM-mediated SAR illustrates the growth versus defense trade-off in plants, as it targets the major virulence factor only under specific biotic stress conditions, thus avoiding the cost of constitutive activation while reducing the damage from virus infection
Evaluating the performance of the LIME and Grad-CAM explanation methods on a LEGO multi-label image classification task
No full textIn this paper, we run two methods of explanation, namely LIME and Grad-CAM, on a convolutional neural network trained to label images with the LEGO bricks that are visible in them. We evaluate them on two criteria, the improvement of the network's core performance and the trust they are able to generate for users of the system. We nd that in general, Grad-CAM seems to outperform LIME on this specic task: it yields more detailed insight from the point of view of core performance and 80% of respondents asked to choose between them when it comes to the trust they inspire in the model choose Grad-CAM. However, we also posit that it is more useful to employ these two methods together, as the insights they yield are complementary.CSE3000 Research ProjectComputer Science and Engineerin
- …
