20 research outputs found
Splice modulation as potential therapy for Usher syndrome type IIa
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199057.pdf (Publisher’s version ) (Open Access)Radboud University, 17 januari 2019Promotor : Kremer, J.M.J. Co-promotores : Wijk, H.A.R. van, Pennings, R.J.E
Towards gene therapy for USH2A-associated retinitis pigmentosa. ~Fishing for answers~
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196854.pdf (Publisher’s version ) (Open Access)Radboud University, 29 november 2018Promotores : Kremer, J.M.J., Keunen, J.E.E. Co-promotor : Wijk, H.A.R. va
Usher syndrome type IIa Genotype-phenotype correlations and hearing
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175288.pdf (Publisher’s version ) (Open Access)Radboud University, 29 september 2017Promotor : Snik, A.F.M. Co-promotores : Pennings, R.J.E., Wijk, H.A.R. va
Molecular genetics of hearing impairment
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147488.pdf (Publisher’s version ) (Open Access)Radboud Universiteit Nijmegen, 04 december 2015Promotor : Kremer, J.M.J. Co-promotores : Schraders, M., Wijk, H.A.R. va
Dissection of the molecular pathology of Usher syndrome.
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80734pub.pdf (Publisher’s version ) (Open Access)RU Radboud Universiteit Nijmegen, 29 mei 2009Promotores : Cremers, C.W.R.J., Cremers, F.P.M. Co-promotores : Kremer, H., Roepman, R.285 p
Energy balance closure for the LITFASS-2003 experiment
No full textIn the first part, this paper synthesises the main results from a series of previous studies on the closure of the local energy balance at low-vegetation sites during the LITFASS-2003 experiment. A residual of up to 25% of the available energy has been found which cannot be fully explained either by the measurement uncertainty of the single components of the surface energy balance or by the length of the flux-averaging period. In the second part, secondary circulations due to heterogeneities in the surface characteristics (roughness, thermal and moisture properties) are discussed as a possible cause for the observed energy balance non-closure. This hypothesis seems to be supported from the fluxes derived from area-averaging measurement techniques (scintillometers, aircraft)
Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus.
No full textThe aim of this study was to identify the receptor(s) for PRRSV on porcine alveolar macrophages (PAMs) by producing monoclonal antibodies (MAbs) against these cells. Hybridoma supernatants were selected for their ability to block PRRSV infection. Four MAbs, 1-8D2, 9.4C7, 9.9F2, and 3-3H2 inhibited infection and recognised cell surface, PAM-specific antigens as shown by immunofluorescence and immunoperoxidase monolayer assay. These MAbs were then used to identify cellular proteins involved in PRRSV infection by radioimmunoprecipitation assays (RIPAs). MAbs 1-8D2 and 9.9F2 each recognised a 150 kDa-polypeptide doublet, while MAbs 9.4C7 and 3-3H2 both recognised a 220 kDa-polypeptide. Glycosidase treatment demonstrated all these polypeptides to be N-glycosylated. Thus, multiple glycoproteins appear to be involved in infection of PAMs by PRRSV
The major envelope protein, GP(5), of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain
No full textA set of neutralizing monoclonal antibodies (mAbs) directed against the GP5 protein of European type porcine reproductive and respiratory syndrome virus (PRRSV) has been produced previously (Weiland et al., 1999). This set reacted with a plaque-purified virus (PPV) subpopulation of Dutch isolate Intervet-10 (I-10), but not with the European prototype PRRSV LV. In order to map the neutralization epitope in the GP5 protein of the PPV strain, the ORF5 nucleotide sequence of PPV was determined. When the amino acid sequence derived from this nucleotide sequence was compared with that of PRRSV LV, four amino acid differences were found. Using site-directed mutagenesis, we showed that a proline residue at position 24 of the GP5 sequence of the PPV strain enabled recognition by the neutralizing mAbs. Pepscan analysis demonstrated that the epitope recognized by the neutralizing mAbs stretched from residues 29 to 35. Surprisingly, the reactivity of the mAbs in the Pepscan system was independent of the presence of a proline in position 24. Moreover, residue 24 is located within the predicted signal peptide, implying that either the signal peptide is not cleaved or is cleaved due to the presence of Pro24 such that the epitope remains intact. Our results demonstrate the presence of a neutralization epitope in the N-terminal ectodomain of the GP5 protein of PRRSV and imply a role for the ectodomain of GP5 in the infection of PRRSV
