1,720,972 research outputs found
Translocation of p190rho guanosine triphosphatase-activating protein from cytosol to the membrane in human neutrophils stimulated with different agonists.
No full textIn this paper, we investigated the subcellular distribution of p190rho guanosine triphosphatase-activating protein (p190 GAP) in human neutrophils stimulated with different agonists. The results show that in neutrophils treated with formyl-methionyl-leucyl-phenylalanine (FMLP) (1) p190 GAP was translocated from the cytosol to the membranes; (2) the translocation of p190 GAP took place only at doses of FMLP that induced the translocation of rac 1 and rac 2 and the activation of the NADPH oxidase; and (3) the kinetic of translocation of p190 GAP paralleled that of rac 1 and rac 2. However, when the agonist was concanavalin A (ConA) or phorbol 12-myristate 13-acetate (PMA), rac 1 and rac 2, but not the p190 GAP, were translocated
The stimulation of human monocytes with interferon-gamma induces the phosphorylation of PU.1 and its binding to gp91phox promoter via activation of a classical protein kinase C
No full textWe previously reported that the stimulation of human blood monocytes with IFN-gamma induces the binding of PU.1 to the gp91phox
promoter and the consequent expression of gp91phox. In this study, we show that the effect of IFN-gamma is reproduced by the serine
phosphatase inhibitor, okadaic acid, and this suggests that serine kinases could be involved in gp91phox expression. We also show
that IFN-gamma induces the serine/threonine phosphorylation of PU.1 in cultured monocytes. This phosphorylation, as well as the
IFN-gamma-induced PU.1 binding and gp91phox protein synthesis, is slightly affected by the casein kinase II inhibitor, daidzein, but is
abrogated by the protein kinase C (PKC) -alpha and -beta inhibitor, Go6976, and by synthetic peptides with sequences based on the
endogenous pseudosubstrate region of the classical PKC alpha and beta isoforms. In contrast, peptides reproducing the pseudosubstrate
region of PKC beta were without effect. Moreover, we found that the treatment of monocytes with IFN-gamma induces the nuclear
translocation and the activation of PKC alpha and betaI, but not of PKC betaII, and that the IFN-gamma-induced phosphorylation of PU.1 was
greatly reduced by LY333531, a selective inhibitor of PKC beta isoforms. Finally, nuclear run-on assays demonstrated that while the
PKC inhibitors, Go6976 and LY333531, decrease the IFN-gamma-induced gp91phox transcription, the serine phosphatase inhibitor,
okadaic acid, enhances the gp91phox gene transcription. Our results indicate that in cultured monocytes, IFN-gamma induces the binding
of PU.1 to the gp91phox promoter and the expression of gp91phox by phosphorylation of PU.1 via activation of PKC alpha and/or beta I
Tyrosine phosphorylation and subcellular redistribution of p125ras guanosine triphosphatase-activating protein in human neutrophils stimulated with FMLP.
No full textIn this paper, we show that the p125 ras guanosine triphosphatase-activating protein (p125 GAP) is present in the cytosol of human neutrophils and is transiently tyrosine phosphorylated and translocated to the membranes upon cell activation with formyl-methionyl-leucyl-phenylalanine (FMLP). When concanavalin A (ConA) or phorbol 12-myristate 13-acetate (PMA), which both induced a long-lasting respiratory burst, were used as stimuli, tyrosine phosphorylation and translocation of p125 GAP did not occur
Inteferon-gamma induces gp91phox expression in human monocytes via protein kinase C-dependent phosphorylation of PU.1.
No full textWe previously reported that the stimulation of human blood monocytes with IFN-gamma induces the binding of PU.1 to the gp91phoxpromoter and the consequent expression of gp91phox. In this study,we show that the effect of IFN-gamma is reproduced by the serine
phosphatase inhibitor,okadaic acid,and this suggests that serine kinases could be involved in gp91phox expression. We also show that IFN-gamma induces the serine/threonine phosphorylation of PU.1 in cultured monocytes. This phosphorylation,as well as the
IFN-gamma-induced PU.1 binding and gp91phox protein synthesis,is slightly affected by the casein kinase II inhibitor,daidzein,but is abrogated by the protein kinase C (PKC) inhibitor Go6976,and by synthetic peptides with sequences based on the endogenous pseudosubstrate region of the classical PKC alpha and beta isoforms. In contrast,peptides reproducing the pseudosubstrate region of PKC epsilon were without effect. Moreover,we found that the treatment of monocytes with IFN-gamma induces the nuclear translocation and the activation of PKC alpha and beta I,but not of PKC beta II,and that the IFN-gamma-induced phosphorylation of PU.1 was greatly reduced by LY333531,a selective inhibitor of PKC beta isoforms. Finally,nuclear run-on assays demonstrated that while the PKC inhibitors,Go6976 and LY333531,decrease the IFN-gamma induced gp91phox transcription,the serine phosphatase inhibitor,enhances the gp91phox gene transcription. Our results indicate that in cultured monocytes,IFN-gamma induces the binding of PU.1 to the gp91phox promoter and the expression of gp91phox by phosphorylation of PU.1 via activation of PKC alpha and/or beta
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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