113 research outputs found
A hybrid feature selection on AIRS method for identifying breast cancer diseases
Get PDFBreast cancer may cause a death due to the late diagnosis. A cheap and accurate tool for early detection of this disease is essential to prevent fatal incidence. In general, the cheap and less invasive method to diagnose the disease could be done by biopsy using fine needle aspirates from breast tissue. However, rapid and accurate identification of the cancer cell pattern from the cell biopsy is still challenging task. This diagnostic tool can be developed using machine learning as a classification problem. The performance of the classifier depends on the interrelationship between sample sizes, some features, and classifier complexity. Thus, the removal of some irrelevant features may increase classification accuracy. In this study, a new hybrid feature selection fast correlation based feature (FCBF) and information gain (IG) was used to select features on identifying breast cancer using AIRS algorithm. The results of 10 times the crossing (CF) of our validation on various AIRS seeds indicate that the proposed method can achieve the best performance with accuracy =0.9797 and AUC=0.9777 at k=6 and seed=50
Chrysin Inhibits Indonesian Serotype Foot-and-Mouth-Disease Virus Replication: Insights from DFT, Molecular Docking and Dynamics Analyses
No full textChrysin, a predominant compound in Propolis, possesses diverse bioactivities, including antiviral properties. However, its antiviral efficacy against the Indonesian Foot-and-Mouth Disease Virus (FMDV) serotype remains unexplored. This study investigates Chrysin's inhibitory potential against FMDV Indonesian serotype by targeting the 3C Protease (3CP), a vital enzyme for viral replication. Multiple sequence alignment was used to reveal unique characteristics of the Indonesian serotype's 3CP compared to global serotypes. Density Functional Theory (DFT) calculations assessed Chrysin's interaction with 3CP based on electronegativity. Molecular docking and molecular dynamics analyses evaluated Chrysin's inhibitory activity against 3CP, using homology modeling for the Indonesian serotype's 3CP structure. Luteolin, a known FMDV 3CP inhibitor with a similar structure to Chrysin, served as a reference. Results showed distinct 3CP sequences in the Indonesian serotype compared to O serotypes and others. Chrysin exhibited potential electron-donor activity with lower HOMO and LUMO values than Luteolin, but they had similar energy gaps, i.e., 4.016 and 4.044 eV, respectively. Molecular docking indicated similar binding affinities, with Chrysin (-6.365 kcal/mol) and Luteolin (-6.864 kcal/mol) bound to active site residues. Molecular dynamics analysis demonstrated stable 3CP-Chrysin and 3CP-Luteolin complexes, with minor differences in Radius of gyration (Rg) and Root-Mean-Square Fluctuation (RMSF) below 1 Å. From the ligand stability point of view, Chrysin had comparable stability with Luteolin. However, Chrysin formed fewer hydrogen bonds and displayed greater free-binding energy than Luteolin during simulation periods. These findings suggest that Chrysin holds promise as an inhibitor of the Indonesian serotype's FMDV 3C Protease.
自然細胞老化およびそのモデルシステム誘導性細胞老化を利用した老化とがんの分子機能解析
No full text筑波大学University of Tsukuba博士（医学）Doctor of Philosophy in Medical Sciences2007【要旨】thesi
As narrativas "japonesas" de Valêncio Xavier : O mistério da prostituta japonesa e Mimi-Nashi-Oichi
Get PDFOrientadora: Profa. Dra. Patrícia da Silva CardosoDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Humanas, Letras e Artes, Programa de Pós-Graduação em Letras. Defesa: Curitiba, 30/03/2012Bibliografia: fls. 84-87Resumo: A novela O mistério da prostituta japonesa & Mimi-nashi-Oichi, escrita por Valêncio Xavier e publicada pela primeira vez em Curitiba, em 1986, pode ser considerada a obra & quot; japonesa" do autor. Embora pareçam duas narrativas distintas, aproximam-se tanto por conterem elementos do universo cultural japonês como por valerem-se de recursos que caracterizam a obra de Valêncio, como a hibridação entre texto e imagem e a inserção de elementos de mistério/fantásticos. Valêncio convida-nos a percorrer os labirintos de seu pensamento inventivo, buscando uma via que concilie a visão e outros sentidos. A interculturalidade e os encontros e desencontros na babel cosmopolita são seu tema. Seu projeto é buscar o Outro, o oposto que será complemento. Sem temer o confronto das diferenças e do estranhamento, o autor avança, buscando a palavra desse Outro. Esta leitura tem como objetivo pensar como o estranho, o mistério e o maravilhoso estão relacionados ao conceito do Outro, considerando O mistério da prostituta japonesa & Mimi-nashi-Oichi uma reflexão sobre a relação entre o Eu familiar do Ocidente e o Outro estranho do Oriente. Para percorrer o labirinto inventivo de Valêncio Xavier, dialogo com Gerard Genette, Julia Kristeva, Walter Benjamim, Haroldo de Campos, Lucia Santaella e Marshall McLuhan e com autores de Estudos Japoneses, como Ernst Fenollosa, Junichiro Tanizaki, Andrew Juniper, Adele Schlombs e os críticos da obra do autor, como Décio Pignatari e outros.Abstract: The novel O mistério da prostituta japonesa & Mimi-nashi-Oichi, written by Valêncio Xavier and first published in Curitiba in 1986, can be considered the Japanese work of the author. Although they seem two separate narratives, they are not worlds apart: both contain aspects of the Japanese cultural universe as well as Valêncio's writing distinctive characteristics, such as hybridization between text and image and insertion of mystery/fantastic elements. Valêncio invites us to walk along the labyrinths of his inventiveness, searching for a way to reconcile vision and other senses. Interculturality, similarities and differences in the cosmopolitan Babel are his subject. The author’s project is the search for the Other, the opposite that will be the complement. Without fearing confrontations, differences and strangeness, Valêncio presses on and seeks the other’s word. This reading aims to meditate on how the weird, the mysterious and the wonderful are related to the concept of the Other, considering O mistério da prostituta japonesa & Mimi-nashi-Oichi a reflection on the relations between the familiar Self from the West and the strange Other from the Far East. In order to navigate through Valêncio Xavier's inventive maze, I have turned to the thoughts of Gerard Genette, Julia Kristeva, Walter Benjamin, Haroldo de Campos, Lucia Santaella and Marshall McLuhan, to the authors of Japanese Studies, as Ernst Fenollosa, Junichiro Tanizaki, Andrew Juniper, Adele Schlombs, as well as to Valêncio Xavier critics, as Décio Pignatari and others
Review: Biology of the commercially used sea urchin Tripneustes gratilla (Linnaeus, 1758) (Echinoidea: Echinodermata)
No full textToha AHA, Sumitro SB, Hakim L, Widodo N, Binur R, Suhaemi, Anggoro AW. 2017. Review: Biology of the commercially used sea urchin Tripneustes gratilla (Linnaeus, 1758) (Echinoidea: Echinodermata). Ocean Life 1: 1-10. Tripneustes gratilla is a species of sea urchin in shallow tropical waters. The species is economically and commercially important, has ecological value, and prospects as a biological control agent. It is considered as the commercially traded sea urchin. Overexploitation has caused a sharp decline in T. gratilla populations. Understanding biological aspects of T. gratilla is critical to the sustainable use of this resource in the future.</jats:p
Oral and intranasal immunization with food-grade recombinant Lactococcus lactis expressing high conserved region of SARS-CoV-2 spike protein triggers mice’s immunity responses
No full textThe COVID-19 pandemic began at the end of 2019 in Wuhan, China, and has spread throughout the world. Vaccination is still the most effective method of prevention of pathogenic infections, including viral infections. However, there is little evidence that vaccination can protect against SARS-CoV-2 virus for a long time. Thus, regular re-vaccination is necessary to control COVID-19. Vaccination by injection is invasive, and is one of the reasons people refuse to get re-vaccinated. Therefore, we developed a less invasive vaccine based on oral or nasal administration. The gene encoding the high conserved region (HCR) spike protein was inserted into pNZ8149 and expressed in L.lactis NZ3900. Mice were immunized at 3-week intervals with oral or nasal routes. Anti-SARS-CoV2 spike antibody (IgG and IgA) level were measured using ELISA method before and after treatment. Plasma cells population in lymph were analyzed using flowcytometry and the CD4 + and CD8 + cells in lymph and intestine were analyzed using immunofluorescence method. The results of nasal and oral administration in experimental animals showed that L.lactis carrying the HCR gene could induce a humoral immune response, as indicated by increased levels of IgG and IgA against SARS-CoV-2 (IgG/IgA-SARS-CoV-2). The plasma cell population after nasal and oral vaccination in mice were significantly different with control group (p < 0.05). The CD4 + and CD8 + cells in intestine were significantly higher in orally immunized group mice than control group. The CD8 + cells in lymph were significantly higher in intranasal immunized group mice than control group. Our data demonstrate L.lactis expressing spike protein can be developed into a less invasive alternative to nasal and oral vaccination
Anticancer potential of holothurin A, holothurin B, and holothurin B3 from the sea cucumber Holothuria scabra
No full textPotential use of compounds from sea cucumbers as MDM2 and CXCR4 inhibitors to control cancer cell growth
No full text<strong>Computational Examination to Reveal Kaempferol as the Most Potent Active Compound from </strong><em><strong>Euphorbia hirta </strong></em><strong>against Breast Cancer by Targeting AKT1 and ERα</strong>
No full textFigure 1: Drug-likeness and toxicity screening. A) Drug-likeness screening using Lipinski, Veber, and Egan rules. B) Toxicity examination based on LD50 and probability to induce toxicity.Figure 2: Screening of membrane permeability and bioactivity. A) Simulation of the chrysin, epicatechin, Kaempferol, quercitol, and syringic acid penetrating the lipid bilayer. B) The energy transfer value of the six compounds penetrating the phospholipid membrane. C) Bioactivity screening. D) The 2D structure of the kaempferol.Figure 3: Prediction of protein targets and functional annotation. A) Prediction of the direct target of the kaempferol compound obtained nine protein target proteins. B) Analysis of target proteins with cBioportal found AKT1 and ERα proteins which have a role in breast cancer progression. C) Proteins that can interact with AKT1 and ERα proteins. D) Functional annotation of target proteins.Figure 4: 3D and 2D visualization of protein-ligand interactions. A) Interaction between AKT1-inhibitor and AKT1-Kaempferol. B) Interaction between ERα-inhibitor and ERα-Kaempferol.Figure 5: Simulation of the molecular dynamics of the interaction between AKT1 and the ligand. A) RMSD backbone proteins. B) RMSD ligand movement. C) Number of hydrogen bonds of the AKT1 protein. D) MM/PBSA binding energy values of the AKT1-inhibitor and AKT1-Kaempferol complexes. E) RMSF values of AKT1-inhibitor and AKT1-Kaempferol complexes.Figure 6: Simulation of the molecular dynamics of the interaction between ERα and ligands. A) RMSD backbone proteins. B) RMSD ligand movement. C) Number of hydrogen bonds of the Akt1 protein. D) MM/PBSA binding energy value of ERα-inhibitor and ERα-Kaempferol complexes. E) RMSF values of ERα-inhibitor and ERα-Kaempferol complexes.Figure 7: Changes in the pose of kaempferol during the simulation result in changes in the number and position of hydrogen bonds. A) AKT1-Kaempferol, B) ERα-Kaempferol.Table 1: Grid coordinate for specific docking.Table 2: Chemical interaction between Akt1 and ERα with the ligands.Table 3: Calculations on binding free energy of the complexesCompound analysis raw data: The result of compound screening in rawFunctional annotation raw data: Raw data of functional annotation resultMolecular docking raw data: The complex of all protein-ligand in this studyRaw data MD: Raw data of all MD simulation</p
Lepidocyrtus (Setogaster) nashi Mateos & Greenslade 2021, sp. nov.
No full textLepidocyrtus (Setogaster) nashi Mateos & Greenslade sp. nov. Figs 23, 24, 35–47, Tab 1 ZooBank: urn:lsid:zoobank.org:act: B8062D31-0B0B-4F0B-971F-EB5EC32ECEF8 Type material. Holotype: female on two slides (head and body separated, code T 22517), Australia, Victoria State, Tagels, Mt Bogong, 1868 m above sea level, S36°41’21” E147°18’24”, in pitfalls under snow in alpine grassland, 14.i.2012, M. Nash leg. Paratypes: one male on slide code T 22518, 4 specimens of unknown sex on slide codes LP307-1, LP307-2, LP307-4, LP307-5, and 2 specimens on absolute alcohol (sample code LP307), same data as holotype. Holotype and paratype slide T 22518 deposited at the Museum of Victoria (Melbourne, Australia); other paratype deposited at the E. Mateos collection (University of Barcelona, Barcelona, Spain). Etymology. The new species was named after the collector, Michael Nash who has been assiduously collecting Collembola for the junior author for many years. Diagnosis. With blue colour present on Ant.I–IV, dorsal and ventral head, Th.II to Abd.IV and coxae, trochanters and femora of three pair of legs; antenna, legs and dorsal side of manubrium unscaled; Ant.IV tip with T-chaeta; eyes G and H strongly reduced; labial chaetotaxy M 1 M 2 rEL 1 L 2 ;dorsal macrochaetae formula A0A2aA2/00/01*00+3 (*short ciliated macrochaeta); without dorsal macrochaetae from Th.II to Abd.I; Abd.I with chaeta a6; Abd.II m3 short ciliated macrochaeta, m5 smooth microchaeta; Abd.III with lateral tuft of 20–25 long ciliated filaments; Abd. IV trichobothria T2 and T4 close to each other, chaetae C1p, T3 and D1p in triangular pattern, chaeta a ciliated and bilobed, chaetae D1 and m ciliated and paddle-like, chaetae F2 and F3 ciliated macrochaete, with two psp on lateral position; unguis with a basal pair of teeth and two small inner teeth; unguiculus lanceolate and with serrated outer margin; spatulate tibiotarsal tenent hair; dentes with small, rounded basal tubercle; mucronal spine with spinelet. Description. Adult body length (without head and furca) 1.0– 1.3 mm (Holotype 1.3). Mesothorax not projecting overhead. Body dorsoventrally compressed. Blue colour present on Ant.I–IV, dispersed spots on dorsal and ventral head, on Th.II to Abd.III, mid-dorsal band on Abd.IV, and coxae, trochanters and femora of three pair of legs; densely black pigmented ocular areas (Fig. 35). Antenna without scales. Antennal length to head diagonal length ratio (head diagonal measured from cervical edge to apex of mouth part) ≈ 1.4. Relation of antennal joints I–IV as 1:1.6:1.7:2.7. Dorsal Ant.I-organ present. Ant. III organ composed of two subcylindrical sensory rods partially covered by an integumentary fold. Ant.IV with subapical mushroom-shaped chaeta (T-chaeta), without apical bulb. Clypeus and labrum as L. agricolus sp. nov. (Fig. 3). Clypeus with four lateral chaetae (2 L1 and 2 L2), four facial chaetae in two rows, and three prefrontal chaetae (1 pf0 and 2 pf1), all these chaetae ciliated. Labrum with ciliated prelabral chaetae and smooth labral chaetae in typical number 4/5,5,4; chaetae of apical row thicker than those in other rows. Closed inverted V-shaped labral apical intrusion; labral papillae smooth. Maxillary palp outer lobe with two subequal smooth chaetae and four smooth sublobal appendages, the tiny distal process that is present in the other two species described above (see Fig. 5) has not been observed (Fig. 36). Lateral process of outer labial papilla short, finger-shape, tip not reaching apex of papilla (Fig. 37). Labium (Figs 38–39) with five smooth proximal chaetae at the base of labial palp; labial anterior row formed by five smooth chaetae (a1–a5); posterior row formed by ciliated chaetae with formula M 1 M 2 rEL 1 L 2 ; r vestigial smooth microchaeta, ratio M 2 /r = 9; postlabial chaetotaxy as in L. agricolus sp. nov. (Fig. 6), with all chaetae ciliated, without spines, 3 chaetae in row I (one paratype asymmetric, with 3+4 ciliated chaetae on this position), 1 chaeta in row C, 3 chaetae in row E, 2 chaetae in row L, and 4 chaetae in row O. Dorsal head (Fig. 40) with macrochaetae A0, A2a and A2; A2a length equal or longer than A2; chaeta Pa5 absent.Interocular chaetotaxy with ciliated chaetae s, t, p, and 3 scales; eyes G and H small and difficult to see on the slides. Th.II–III dorsal chaetotaxy as L. agricolus sp. nov. (Figs 8–9). Th.II with 2 lateral S-chaetae (al and ms) and without macrochaetae in dorsal position. Th.III with a lateral sensillum (al) close to several ciliated chaetae. Abd.I dorsal chaetotaxy as in Fig. 41, with chaeta m5 and a lateral S-microchaeta (ms) external to a6. Abd.II chaetotaxy as in Fig. 42; chaetae ml and a2p absent; chaetae mi, a2, ml and ll fan-shaped; chaeta m3 short ciliated macrochaeta, ratio m3/m3e = 1.2; chaeta a6 present; m5 smooth microchaeta. Abd.III chaetotaxy as in Fig. 43; with S-chaetae as and ms; chaeta d3 present on one specimen, absent on the rest; chaetae mi, ml, a2, li, lm, ll, a6, im, em and am6 fan-shaped, of which a6 and li larger (paddle-like); p6 and pm6 broad ciliated macrochaetae; a8 and p8 thin ciliated macrochaetae; with lateral tuft of 20–25 long ciliated filaments. Abd.IV chaetotaxy as in Fig. 44; Sm smooth microchaeta; B4 and B5 broad ciliated macrochaetae, B6 thin ciliated macrochaeta with socket of minor diameter than macrochaetae B4 and B5; F2 broad ciliated macrochaeta; F3 thin ciliated macrochaeta; Fe4 thin ciliated macrochaeta; with 4+4 dorsal long S-chaetae on anterior region; with two lateral psp located external to chaeta r4. In the Abd.IV anterior bothriotrichal complex the ratio of distances between T2–T4/C1p ≈ 3; bothriotrichum T2 without accessory chaeta s; chaetae C1p, D1p and T3 forming a triangle (as in L. agricolus sp. nov., Fig 17); chaeta C1p strongly ciliated (Holotype) or finelly cilated (Paratypes), chaetae m, D1, pi and pe fan-shaped, from which m and D1 larger (paddle-like); chaeta a bilobed and cliated. Abd.V (Fig. 45) with dorsal S-chaetae as, acc.p4 and acc.p5. (+)––present, (-)––absent,?––no data available, smic––smooth microchaeta, tcmac––thin cliliated macrochaeta, cmac––cliliated macrochaeta, bil––bilobed, sim––simple (not bilobed), Ceph: cephalic chaetae, Abd.III tuft: number of long filaments in lateral Abd.III tuft, Unguis bp%: basal pair of teeth position on the inner edge of unguis-III measured in percentage from base of this inner edge; Unguiculus: morphology of unguiculus apex, acum––acuminate, trunc––truncate; Dental tubercle: basal dental tubercle absence/presence and morphology. a Data from Mateos & Grenslade (2015). b Zhi Xiang Pan personal communication. Ventral tube without scales, with 10+10 ciliated chaetae on anterior side and 9 ciliated chaetae on posterior side (as in L. agricolus sp. nov., Fig 19); all chaetae on posterior side ciliated; each lateral flap with maximum of 6 ciliated chaetae and 6 smooth chaetae. Legs without scales. V shaped trochanteral organ (leg III) with maximum of 15 smooth straight chaetae arranged in triangular shape (Fig. 46). Unguis (Fig. 47) with well-developed basal pair of teeth at 42% from base of the inner edge, and with two small inner teeth at 67% and 84% from base of inner edge, respectively (apical tooth smaller); unguiculus lanceolate and with serrated outer margin; spatulate tibiotarsal tenent hair and acuminate supra-empodial chaeta; ratio tenent hair/supra-empodial chaeta ≈ 2; ratio unguis inner edge/tenent hair ≈ 1. Manubrium without scales on dorsal surface; ventro-apical end with 2+2 ciliated chaetae; manubrial plate with two psp, 3 inner chaetae, and 3 outer chaetae. Dentes with small, rounded basal tubercle (difficult to see in several specimens). Mucronal basal spine with spinelet (as in L. agricolus sp. nov., Fig. 22). Pseudopores distribution as in L. (S.) agricolus sp. nov. (Figs 23–24). Discussion. Lepidocyrtus (Setogaster) nashi sp. nov. is close to the other two new species described, L. (S.) agricolus sp. nov. and L. (S.) coorongensis sp. nov., and to the other species listed in Table 1. Body with blue pigment and labium with smooth vestigial microchaeta r are good diagnostic characters differentiating L. (S.) nashi sp. nov. from species L. (S.) agricolus sp. nov., L. (S.) coorongensis sp. nov. and L. (S.) kuakea (Table 1). Acuminate unguiculus in L. (S.) nashi sp. nov. clearly separates this species to the other pigmented species listed in Table 1. The new species appears to be locally endemic and restricted to alpine grassland habitat on Mt Bogong where it is active under snow. The plant species present in the area were Celmisia costiniana (70%), Poa fawcettiae (20%), Luzula acutifolia (5%), and assorted herbs (5%) including Trachymene humilis and Aciphylla glacialis. A number of other invertebrates have been described as restricted to this habitat and locality (Greenslade & Slatyer 2017, Braby & Wurtz 2018). Rocky crevices here are an aestivation site for the Bogong Moth, an important food source for the Mountain Pygmy Possum. This fauna is being impacted adversely by climate change resulting in reduced snow cover as well as by increasing tourism. There is anecdotal evidence of visitors interfering with aestivating moths.Published as part of Mateos, Eduardo & Greenslade, Penelope, 2021, Towards understanding Lepidocyrtus Bourlet, 1839 (Collembola, Entomobryidae) II: new Australian species, pp. 365-387 in Zootaxa 4981 (2) on pages 378-383, DOI: 10.11646/zootaxa.4981.2.9, http://zenodo.org/record/492097
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