475 research outputs found
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Exploring stem cell therapies: an interview with Darius Widera
Full text linkEditorial: Repair and regenerative mechanisms by stem/progenitor cells and secretome: The golden twenties of translational medicine
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Origin and regenerative potential of vertebrate mechanoreceptor-associated stem cells
Full text linkWidera D, Hauser S, Kaltschmidt C, Kaltschmidt B. Origin and regenerative potential of vertebrate mechanoreceptor-associated stem cells. Anatomy Research International. 2012;2012:1-9.Meissner corpuscles and Merkel cell neurite complexes are highly specialized mechanoreceptors present in the hairy and glabrous skin, as well as in different types of mucosa. Several reports suggest that after injury, such as after nerve crush, freeze injury, or dissection of the nerve, they are able to regenerate, particularly including reinnervation and repopulation of the mechanoreceptors by Schwann cells. However, little is known about mammalian cells responsible for these regenerative processes. Here we review cellular origin of this plasticity in the light of newly described adult neural crest-derived stem cell populations. We also discuss further potential multipotent stem cell populations with the ability to regenerate disrupted innervation and to functionally recover the mechanoreceptors. These capabilities are discussed as in context to cellularly reprogrammed Schwann cells and tissue resident adult mesenchymal stem cells
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Signaling via NF-kappa B in the nervous system
No full textKaltschmidt B, Widera D, Kaltschmidt C. Signaling via NF-kappa B in the nervous system. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 2005;1745(3):287-299
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Potential role of NF-kappa B in adult neural stem cells: the underrated steersman?
No full textWidera D, Mikenberg I, Kaltschmidt B, Kaltschmidt C. Potential role of NF-kappa B in adult neural stem cells: the underrated steersman? INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE. 2006;24(2-3):91-102
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Improved method for ex ovo-cultivation of developing chicken embryos for human stem cell xenografts
Full text linkSchomann T, Qunneis F, Widera D, Kaltschmidt C, Kaltschmidt B. Improved method for ex ovo-cultivation of developing chicken embryos for human stem cell xenografts. Stem Cells International. 2013;2013: 960958.The characterization of human stem cells for the usability in regenerative medicine is particularly based on investigations regarding their differentiation potential in vivo. In this regard, the chicken embryo model represents an ideal model organism. However, the access to the chicken embryo is only achievable by windowing the eggshell resulting in limited visibility and accessibility in subsequent experiments. On the contrary, ex ovo-culture systems avoid such negative side effects.
Here, we present an improved ex ovo-cultivation method enabling the embryos to survive 13 days in vitro. Optimized cultivation of chicken embryos resulted in a normal development regarding their size and weight. Our ex ovo-approach closely resembles the development of chicken embryos in ovo, as demonstrated by properly developed nervous system, bones, and cartilage at expected time points. Finally, we investigated the usability of our method for trans-species transplantation of adult stem cells by injecting human neural crest-derived stem cells into late Hamburger and Hamilton stages (HH26–HH28/E5—E6) of ex ovo-incubated embryos. We demonstrated the integration of human cells allowing experimentally easy investigation of the differentiation potential in the proper developmental context. Taken together, this ex ovo-method supports the prolonged cultivation of properly developing chicken embryos enabling integration studies of xenografted mammalian stem cells at late developmental stages
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Methods for the Modulation and Analysis of NF-kappaB-dependent Adult Neurogenesis
No full textWidera D, Müller J, Imielski Y, Heimann P, Kaltschmidt C, Kaltschmidt B. Methods for the Modulation and Analysis of NF-kappaB-dependent Adult Neurogenesis. Journal of Visualized Experiments. 2014;(84):e50870
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NF-kB, Potential Role in Adult Neural Stem Cells
No full textWidera D, Kaltschmidt C, Kaltschmidt B. NF-kB, Potential Role in Adult Neural Stem Cells. In: Marc B, Nobutaka H, Uwe W, eds. Encyclopedia of Neuroscience. Berlin: Springer Verlag; 2009: 2862-2867
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TNF-alpha mediated transport of NF-kappa B to the nucleus is independent of the cytoskeleton-based transport system in non-neuronal cells
No full textMikenberg I, Widera D, Kaltschmidt B, Kaltschmidt C. TNF-alpha mediated transport of NF-kappa B to the nucleus is independent of the cytoskeleton-based transport system in non-neuronal cells. EUROPEAN JOURNAL OF CELL BIOLOGY. 2006;85(6):529-536
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Role of NF-kappaB in brain function
No full textKaltschmidt B, Mikenberg I, Widera D, Kaltschmidt C. Role of NF-kappaB in brain function. In: G. T, ed. Transcription Factors in the Nervous System. Weinheim: Willey-VCH; 2005: 327-343
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