1,721,307 research outputs found
Perpetual sedimentation open instrumentation
No full textThis page describes how to build rotating syringe pumps suitable for Drop-seq microfluidics and many other applications.</span
ECDM methods for fluidic interfacing through thin glass substrates and the formation of spherical microcavities
No full textElectrochemical discharge machining (ECDM) involves the electrolytic formation of a gas film at a tool electrode with high current density discharges and Joule heating for local material heating and removal. The ECDM process is ideally suited for low density glass through-hole machining for applications such as fluidic interconnection. In this paper, we describe a simple and robust ECDM cell arrangement and present optimum conditions for rapid and reproducible through-hole machining in both 500 mu m thick and fragile 180 mu m thin borosilicate glass substrates. Both anodic and cathodic methods were evaluated and the results offer additional insight into the complex and polarity-dependent mechanisms involved in the ECDM process. The anodic process produces unique spherical cavity microstructures, presenting a new capability for glass microfabrication
Ultrafast cell switching for recording cell surface transitions: new insights into epidermal growth factor receptor signalling
Full text linkA pinched-flow deflection technology was developed for rapidsingle cell switching between biochemical microenvironments.Millisecond switching was used to stimulate and preserve epidermal growth factor receptor (EGFR) autophosphorylation transitions. Intramolecular phosphorylation initiates signal transduction, is silenced by phosphatase activity until EGFR dimerization enables intermolecular phosphorylation to initiate downstream signalling
Droplet Kitchen
No full textOpen Instrumentation and Howtos for Microfluidics: Flexible and affordable droplet microfluidics with digital fabrication, open hardware and open source software.</span
Emerging technologies for understanding platelet diversity
No full textThis review discusses our understanding of platelet diversity with implications for the roles of platelets in hemostasis and thrombosis and identifies advanced technologies set to provide new insights. We use the term diversity to capture intrasubject platelet variability that can be intrinsic or governed by the environment and lead to a heterogeneous response pattern of aggregation, clot promotion, and external communication. Using choice examples, we discuss how the use of advanced technologies can provide new insights into the underlying causes of platelet molecular, structural, and functional diversity. As sources of diversity, we discuss the proliferating megakaryocytes with different allele-specific expression patterns, the asymmetrical formation of proplatelets, changes in platelets induced by aging and priming, interplatelet heterogeneity in thrombus organization and stability, and platelet-dependent communications. We provide indications how current knowledge gaps can be addressed using promising technologies, such as next-generation sequencing, proteomic approaches, advanced imaging techniques, multicolor flow and mass cytometry, multifunctional microfluidics assays, and organ-on-a-chip platforms. We then argue how this technology base can aid in characterizing platelet populations and in identifying platelet biomarkers relevant for the treatment of cardiovascular disease
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Modelling annular micromixers
No full textMagnetohydrodynamic mixing of two fluids in an annular microchannel is modelled as a two-dimensional laminar convection-diffusion problem and examined using asymptotic analysis and numerical simulation. The time T required for mixing of a plug of solute depends on the Peclet number Pe and on the geometry of the annulus. Three scaling regimes are identified: purely diffusive, Taylor-dispersive, and convection-dominated; each has a characteristic power-law dependence of T upon Pe. Consequences of these results for optimal micromixer design are discussed
- …
