49,542 research outputs found
Wergeland : En historisk djupdykning och renovering av Carl Malmstens soffa Wergeland
No full textSoffan Wergeland, ritad 1944 av möbelformgivaren Carl Malmsten, har ett traditionellt knutet resårhus och delad framkant. I studien renoveras en soffa av modellen Wergeland och här provas ett nytt sätt att knyta resårer, som visar sig vara tidseffektivt. Att tillverka en delad framkant med vulster på traditionellt sätt är tidskrävande och krångligt och därför används en förenklad metod att bygga upp vulster med hjälp av pressat papper. Metoden ger ett bra resultat och är till utseende jämförbart med det traditionella sättet. Modellen Wergeland har fått sitt namn efter beställaren Hjalmar Wergeland, läkare och norsk motståndsman under andra världskriget, som tvingas fly till Sverige och tillsammans med sin fru Helga bygger de upp ett nytt hem i Stockholm och beställer en soffa av Carl Malmsten. I studien presenteras historiska fakta från 1940-talet då soffan beställs, om Carl Malmsten, Hjalmar och Helga Wergeland och dokument, ritningar och en brevväxling mellan Hjalmar Wergeland och Carl Malmsten
The construction of Karen Karnak: The multi-author-function
No full textThis thesis is situated within the comparatively recent developments of Web 2.0 and the emergence of interactive WikiMedia, and explores the mode of authorship within a Read/Write culture compared to that of a Read/Only tradition. The hypothesis of this study is that the role of the audience has become merged with the author, and as such, represents new functions and attributes, distinct from a more conventional concept of authorship, in which the roles of audience and author are more separate. Read/Write and participatory culture, as defined by this study, is focused on collaboration, and includes the influences of D.I.Y. culture, Open-Source practices and the production of text by multiple authors. Multi-authorship presents a re-thinking of several concepts which support the notion of the individual author, since the focus of multi-authorship is not on attribution and ownership of a finished text, but on the continued malleability of a text. Modes of multi-authorship, demonstrated in the use of the pseudonyms Alan Smithee and Karen Eliot, represent declarative authors whose names signify multiple origins, whilst concurrently indicating a distinct body of work. The function of these names form an important context to this study, since primary research involves the construction of an experimental mode of multi-authorship utilising WikiMedia technology and the interaction of thirty nine participants, who are invited to create a body of work under the collective pseudonym Karen Karnak. The data generated by this experiment is analysed using aspects of Michel Foucault's author-function to identify and determine power structures inherent in the WikiMedia context. The interplay of power structures, including concepts such as identity, ownership and the body of work, affect the resulting mode of authorship and contribute to the construction of Karen Karnak, suggesting further areas of research into the emerging multi-author
Responding to lethal violence: RCMP use of deadly force
No full textNot peer reviewedContemporary police officers face the challenge of intervening in community crises while maintaining public and personal safety. Unfortunately, this sometimes includes precarious life and death encounters which require using deadly force.police; RCMP; use of force; deadly force; mental illness; crisis interventio
Tagging of Biomedical Articles on CiteULike: A Comparison of User, Author and Professional Indexing
No full textThis paper examines the context of online indexing from the viewpoint of three different groups: users, authors, and professional indexers. User tags, author keywords and descriptors were collected from academic journal articles, which were both indexed in Pubmed and tagged on CiteULike, and analysed. Descriptive statistics, informetric measures, and thesaural term comparison shows that there are important differences in the use of keywords between the three groups in addition to similarities which can be used to enhance support for search and browse. While tags and author keywords were found that matched descriptors exactly, other terms which did not match but provided important expansion to the indexing lexicon were found. These additional terms could be used to enhance support for searching and browsing in article databases as well as to provide invaluable data for entry vocabulary and emergent terminology for regular updates to indexing systems. Additionally, the study suggests that tags support organisation by association to task, projects and subject while making important connections to traditional systems which classify into subject categories
Application of in vitro transcribed and translated HIV-1 reverse transcriptase and integrase in protein-protein interaction studies
No full textReplication of the human immunodeficiency virus (HIV) necessitates the two viral enzymes reverse transcriptase (RT) and integrase (IN). These two proteins are found to associate in a large nucleoprotein complex called the preintegration complex (PIC) together with viral nucleic acids and other viral and host proteins. Since the two proteins are localised together in the host cell, a direct interaction between them can exist, although it has been difficult to demonstrate such interaction. In this study, RT and IN were produced by in vitro transcription and translation and the proteins were used to study possible RT-IN interactions by ELISA. The RT and IN coding regions were amplified by PCR from a full length HIV-1 HxB2 clone and ligated in-frame into the in vitro translation vector pCITE-5b(+). The two plasmids generated, pCITE-RT and pCITE-IN were constructed and verified by sequencing and restriction enzyme analysis. A coupled in vitro transcription and translation kit based on rabbit reticulocyte lysate was utilised together with the two plasmids in order to obtain RT and IN. The proteins were produced unmarked since marked amino acids possibly could obscure an eventual interaction. Verification of the protein products was done by Western blotting having specific monoclonal antibodies. The proteins produced were not purified from the reticulocyte lysate but used directly in an ELISA-format to try to detect any interactions between RT and IN. With the ELISA-format, no interactions could be demonstrated. The signal was the same for all dilutions of the coating protein, as well as for the negative control. The most likely explanation for this is that there are some proteins in the reticulocyte lysate that interacts with both RT and IN. If in vitro translated proteins are going to be used to investigate protein-protein interactions by this ELISA method they need to be purified first. An other possibility is to cotranslate the two proteins in vitro. It is not sure that an eventual direct interaction between RT and IN can be detected by ELISA. Other components of the PIC may need to be present for a direct interaction to occur
Flt3 receptor tyrosine kinase in AML and its modulation of the p53/Hdm2/Bcl-2 pathway
No full textAcute myeloid leukemia (AML) is an aggressive disease with a poor overall survival rate. The most frequent mutations associated with AML are internal tandem duplications (ITD) in the juxtamembrane region of the receptor tyrosine kinase Flt3. These mutations render the receptor constitutively active and alter signaling trough FLT3-ITD compared to wild type receptor. Non-mutated TP53 and elevated expression of its regulator Hdm2 is another interesting feature of AML. Studies have shown that the p53 pathway can be linked to Flt3 signaling as a subset of FLT3-ITD patients expresses increased levels of the anti-apoptotic protein Bcl-2 and hyperphosphorylated p53 protein. This thesis investigates the properties of FLT3-ITD and the interconnection of the Flt3 signaling pathway with the p53/Hdm2/Bcl-2 pathway in an attempt to elucidate novel therapeutic targets in AML. The experiments demonstrate a reciprocal regulation of Flt3 and Hdm2 in AML cells with Flt3-wt while FLT3-ITD cells suspend the Hdm2 modulation. FLT3-ITD patient cells have a higher level of Flt3 protein and the ITD results in dysregulated receptor turnover and attenuated Hdm2 down-regulation. Thus targeting of FLT3-ITD may result in elevated Hdm2 and increased tolerance for p53. The involvement of Bcl-2 family of proteins is also seen in this setting; a persistent attenuation of Mcl-1 is required for the cells to undergo apoptosis, however this is only seen in Flt3-wt cells. Hdm2 is in addition shown to be important as a regulator of Bcl-2. The blockage of Hdm2 E3 ligase activity results in increased Bcl2 and Hdm2 is required for Bcl-2 protection from p53-induced cell death. To conclude, wild type and mutated Flt3 is interconnected with the important p53/Hdm2/Bcl-2 pathway at several levels. Therapeutic targeting of Flt3 should therefore be evaluated in co-operation with the p53/Hdm2/Bcl-2 pathway. Combined targeting of FLT3-ITD, Hdm2 and Bcl-2 may therefore be interesting when approaching the development of novel AML therapy
Second Author Affiliation / Address line 1 Affiliation / Address line 2
No full textThis document contains the instructions for preparing a camera-ready manuscript for the proceedings of ACL-2015. The document itself conforms to its own spec-ifications, and is therefore an example of what your manuscript should look like. These instructions should be used for both papers submitted for review and for final versions of accepted papers. Authors are asked to conform to all the directions re-ported in this document.
Droplet motion with contact-line friction: long-time asymptotics in complete wetting
No full textWe consider the thin-film equation for a class of free boundary conditions modelling friction at the contact line, as introduced by E and Ren. Our analysis focuses on formal long-time asymptotics of solutions in the perfect wetting regime. In particular, through the analysis of quasi-self-similar solutions, we characterize the profile and the spreading rate of solutions depending on the strength of friction at the contact line, as well as their (global or local) corrections, which are due to the dynamical nature of the free boundary conditions. These results are complemented with full transient numerical solutions of the free boundary problem. Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Mathematical Physic
A study of the level of instructor interest and experience concerning the development of on-line courses at Wisconsin Indianhead Technical College
No full textPlan BThis study examined the level of interest and experience concerning on-line course development among full-time instructors employed by the Wisconsin Indianhead Technical College. The purpose of the study is to determine level of interest concerning the development of on-line courses, the level of experience to develop and instruct on-line courses, and the need for training and support for on-line course development. Recommendations of this study will be used to help WITC develop a sense of direction for future on-line course development. It may also provide the necessary information needed to create and provide on-line course development training, curriculum guidelines and standards, and the collection and compilation of developmental resource materials. Since the platform for on-line learning is through the Internet, an electronic on-line survey was chosen as the means of collecting data. During the Spring Semester of 2003, an e-mail message containing a link to access the survey was sent to all full-time WITC instructors. After each instructor completed the survey, the data was sent electronically via the Internet, without any personal identifiers, to University of Wisconsin-Stout Publications for processing. Recommendations of this study will be used to help WITC develop a sense of direction for future on-line course development. It may also provide the necessary information needed to create and provide on-line course development training, curriculum guidelines and standards, and the collection and compilation of developmental resource materials
Line transect sampling of primates : can animal-to-observer distance methods work?
No full textAn erratum to this article can be found at http://dx.doi.org/10.1007/s10764-010-9469-4Line transect sampling is widely used for estimating abundance of primate populations. Animal-to-observer distances (AODs) are commonly used in analysis, in preference to perpendicular distances from the line. This is in marked contrast with standard practice for other applications of line transect sampling. We formalize the mathematical shortcomings of approaches based on AODs, and show that they are likely to give strongly biased estimates of density. We review papers that claim good performance for the method, and explore this performance through simulations. These confirm strong bias in estimates of density using AODs. We conclude that AOD methods are conceptually flawed, and that they cannot in general provide valid estimates of density.Peer reviewe
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