31,086 research outputs found
Electro-microfiltration treatment of water containing natural organic matter and inorganic particles
No full textMorphological and molecular identification of leptocephali of Taiwanese duckbill conger, Gavialiceps taiwanensis (Chen & Weng, 1967) (Family Congridae)
No full textLin, Chien-Ting, Ho, Hsuan-Ching (2018): Morphological and molecular identification of leptocephali of Taiwanese duckbill conger, Gavialiceps taiwanensis (Chen & Weng, 1967) (Family Congridae). Zootaxa 4454 (1): 215-227, DOI: 10.11646/zootaxa.4454.1.1
The recognition region in SulA by ClpYQ protease from Escherichia coli
No full text大腸桿菌中ClpYQ蛋白酶為一種ATP依賴蛋白酶，由具有ATPase及unfoldase活性的ClpY，與具有peptidase活性的ClpQ所構成的雙單元體。這類蛋白酶在細胞中，可降解構形錯誤或是具危害性的蛋白質，以維持細胞正常生理作用，避免細胞受到危害。在ClpYQ蛋白酶中，ClpY會負責辨識基質，並水解ATP作為能量來源，將基質結構打開並傳送至ClpQ的活性區，以進行降解作用。然而，關於ClpYQ蛋白酶是如何選擇辨識基質，及後續降解作用的詳細機制，目前仍不清楚。SulA是一個細胞分裂的抑制物，當細胞暴露在逆境下時會產生SOS反應，誘導大量SulA蛋白表現，以避免受損的DNA傳到子代細胞。目前已知可分解SulA的蛋白酶為Lon及ClpYQ，其中又以Lon為主要負責分解的蛋白酶。之前有研究指出，Lon可以藉由辨認SulA之C-端末8個胺基酸，與之結合並將其降解，但是ClpYQ卻不能。對於ATP依賴蛋白酶來說，為避免不必要的降解，如何選擇辨認需要降解的基質是非常重要的。本實驗中，為確認ClpYQ蛋白酶辨認SulA蛋白的區域，建構SulA之C-端不同大小片段缺失的突變蛋白，以酵母菌雙雜交系統測試，各個SulA突變蛋白與ClpY之間交互作用的情形，發現ClpY辨識的區域可能位於C-端的高疏水性片段，C-端第20 - 30個胺基酸。於此區域內再建構點突變蛋白，分析不同性質的胺基酸對於ClpY交互作用的影響，結果顯示當點突變取代為親水性胺基酸時，會降低SulA蛋白與ClpY之間的交互作用。之後測試各個SulA突變蛋白的活性表現，及被ClpYQ蛋白酶降解之情形，結果顯示SulA之C-端第20 - 45個胺基酸的區域，對於其抑制細胞分裂的活性表現是重要的，且對於ClpYQ蛋白酶的降解作用也會造成影響，因此ClpYQ蛋白酶應可藉由SulA蛋白之活性表現與否，來辨別其是否需要降解。ClpYQ is an ATP-dependent protease from Escherichia coli and a two component complex composed of ClpY, which is an ATPase and unfoldase, and ClpQ peptidase. Degradation of denatured or damaged proteins by this proteases helps protect the normal cell growth from the harmful effects of these proteins. The ClpY is thought to recognize protein substrates, denature them, and translocate the unfolded polypeptide into the catalytic cavity of the ClpQ for degradation. However, little information is available on the recognition of substrates for ClpYQ and on the mechanism by which they were selected, unfolded, and translocated by ClpY to the interior of the ClpQ. SulA, induced in the SOS response, is a cell division inhibitor and prevents the distribution of damaged DNA into daughter cells during DNA repair processes. SulA can be degraded by ATP-dependent proteases such as Lon and ClpYQ, and the degradation in vivo seems to be predominantly by Lon, while ClpYQ appears to act as a backup for Lon. It was reported previously that the region of C-terminal eight amino acid residues of SulA was essential for interaction with Lon but not with ClpYQ. To avoid unnecessary degradation of cellular proteins, substrate selection by ATP-dependent proteases is tightly regulated; therefore, it is interesting to investigate the recognition region of SulA by ClpYQ. In this study, the deletion mutants of SulA with regard to C-terminus were constructed and the interaction with ClpY was analyzed in yeast two-hybrid system. The results showed that ClpY recognized the hydrophobic region of SulA, C20 - 30 aa, and the recognition was also likely to rely on hydrophobic interaction following the observation that the binding activity decreased if the substituted residue was polar. The C-terminal region of SulA, C20 - 45 aa, seemed to be important for its activity with an inhibition of cell division, and the region is necessary for the degradation by ClpYQ. Therefore, ClpYQ protease would be able to distinguish whether SulA is to be degraded by the activity of inhibition.目錄試委員會審定書 i謝 ii要 iiibstract iv錄 vi目錄 viii目錄 ix圖目錄 x、前言 1、ATP依賴蛋白酶 1、ClpYQ蛋白酶 2、ClpYQ複合體結構 4、ClpYQ蛋白酶之基質 5、細胞分裂抑制物SulA 7、蛋白酶對基質SulA辨認之相關研究 9、研究動機與目的 12、材料與方法 13、實驗材料 13一) 菌株與質體 13二) 藥品與試劑 14三) 器材設備 15四) 分析軟體 15、實驗方法 16一) 一般實驗方法 16二) 菌體的建構 20三) 突變基因建構 25四) 酵母菌雙雜交系統分析 28五) 大腸桿菌選殖基因表現系統 33六) 西方墨點分析 (Western Blotting) 35、實驗結果 41、確認SulA蛋白被ClpY辨識之區域 41一) 建構SulA蛋白C-端不同大小片段缺失之突變 41二) 以酵母菌雙雜交系統測試各個SulA突變蛋白與ClpY之交互作用 41、SulA蛋白被辨識區域之特性 43一) SulA蛋白C-端序列特性分析 43二) 於SulA蛋白被辨識區域中建構不同胺基酸性質之點突變 43三) 利用酵母菌雙雜交系統測試SulA點突變蛋白與ClpY之交互作用 44、不同蛋白酶對於SulA蛋白辨識區域之差異 45一) Lon蛋白酶與SulA缺失突變蛋白間之交互作用 45二) ClpY I domain上兩個loop對於SulA蛋白辨識之影響 46、SulA突變蛋白之活性表現及受ClpYQ蛋白酶降解之現象 47一) C-端末20個胺基酸缺失之SulA突變蛋白 48二) C-端二級結構部份缺失之SulA突變蛋白 50三) SulA不同性質胺基酸之點突變蛋白 51、討論 53、SulA蛋白被ClpY辨識之區域及其特性 53、不同蛋白酶對於辨認相同基質之差異 54、SulA突變蛋白之活性表現與FtsZ的相關性 55、ClpYQ蛋白酶對於SulA突變蛋白之降解情形 57、質體SulA的表現會誘導染色體之SulA表現 58、結論 60、參考文獻 61目錄一、本論文所使用的菌株與噬菌體 67二、本論文所使用的質體 68三、本論文所使用的引子對 69四、在酵母菌雙雜交系統中SulA突變蛋白與ClpY之交互作用情形 71五、SulA突變蛋白之活性測試及受ClpYQ蛋白酶降解之情形 72目錄一、大腸桿菌之SulA結構圖 73二、以酵母菌雙雜交系統分析Sul
Fagopyrum longistylum (Polygonaceae), a new species from Sichuan, China
No full textZhang, Kaixuan, Fan, Yu, Weng, Wenfeng, Tang, Yu, Zhou, Meiliang (2021): Fagopyrum longistylum (Polygonaceae), a new species from Sichuan, China. Phytotaxa 482 (2): 173-182, DOI: 10.11646/phytotaxa.482.2.5, URL: http://dx.doi.org/10.11646/phytotaxa.482.2.
Neenchelys cheni Chen & Weng 1967
No full text* Neenchelys cheni (Chen & Weng, 1967) RR*ª Myrophis cheni Chen & Weng, 1967: 39 (Type locality: Tungkang [Dong-gang], southwestern Taiwan). Chen, 1969: 130; Shen 1984 a: 117; Chen & Yu, 1986: 258; Shen et al., 1993: 112; Shao et al., 2008: 238. " Neenchelys " cheni (Chen & Weng, 1967): Ho et al. 2010: 24, table 1; Ho & Shao, 2011: 23, table 1. Neenchelys cheni (Chen & Weng, 1967): Hibino et al., 2012: 345; Ho et al., 2013: 5. Neenchelys retropinna Smith & Böhlke, 1983: McCosker & Chen, 2000: 356. Remarks. An uncommon species collected by midwater trawls off southwestern Taiwan.Published as part of Ho, Hsuan-Ching, Smith, David G., Mccosker, John E., Hibino, Yusuke, Loh, Kar-Hoe, Tighe, Kenneth A. & Shao, Kwang-Tsao, 2015, Annotated checklist of eels (orders Anguilliformes and Saccopharyngiformes) from Taiwan, pp. 140-189 in Zootaxa 4060 (1) on pages 169-170, DOI: 10.11646/zootaxa.4060.1.16, http://zenodo.org/record/24365
A Newly Commissioned Work for Cello, A Recording and Performance Practice Guide by Yu-Ting Tseng
No full textabstract: The introduction of a new instrumental piece—specifically Taiwanese—into the cello repertoire is as exciting as it is important. Currently, the majority of works for cello and piano include predominantly Western compositions that is repeatedly taught and performed. Reflections, by Taiwanese composer Ming-Hsiu Yen (Ms. Yen) is a response to this saturation. It is a piece that is both demanding for the performers and entertaining for the audience. Brilliantly written by a composer who has intimate familiarity with both the cello and piano, it is highly suitable for scholarly study and performance.
This document details ensemble issues, interpretative suggestions for both cellist and pianist, and general concepts about the music. The composer further adds to these concepts and suggestions.
Reflections is a programmatic work comprised of four movements, each with a descriptive title: “Gear,” “Tears of the Angel,” “Spintop,” and “Transformation.” Because the composer’s intentions were driven by pictorial ideas and not by a formal harmonic structure, this paper concentrates on ensemble issues and interpretation less than harmonic analysis.
Secondly, the project includes the premiere recording of Reflections, as performer by Yu-Ting Tseng, cellist, and Dr. Jeremy Peterman, pianist. This audio documentation provides other cellists and pianists the opportunity of hearing the piece as originally conceived by the composer, as an aid to their own future preparation of this work. This recording, combined with the interpretative analysis, will assist in bringing Reflections into the cello repertoire and public eye.Dissertation/ThesisReflections Recording Movement I GearReflections Recording Movement II Tears of the AngelReflections Recording Movement III SpintopReflections Recording Movement IV TransformationDoctoral Dissertation Music 201
Lun yu hui han /
No full text"Ming Tianqi yi chou (5 nian) chong kan ben"1. Lun yu gu xun. -- 2. Lun yu shi yi / Su Che. -- 3. Kong zi Lun yu nian pu / Cheng Fuxin. -- 4. Lun yu fu ji / Weng Fanggang. -- 5. Lun yu fa yin / Yang Wenhui
Xiao shi fan ting wu yan shi xu chao: [8 juan, juan shou]. v.62
No full text翁方綱, [伍崇曜]輯.Date from preface.框13.1 x 9.1 cm., 9行21字, 黑口, 左右雙邊, 無魚尾, 版心中鐫分冊書名, 下鐫叢書名.Weng Fanggang, [Wu Chongyao] ji.Kuang 13.1 x 9.1 cm., 9 xing 21 zi, hei kou, zuo you shuang bian, wu yu wei, ban xin zhong juan fen ce shu ming, xia juan cong shu ming
Gavialiceps taiwanensis Chen & Weng 1967
No full text* Gavialiceps taiwanensis (Chen & Weng, 1967) a*ªffiª Chlopsis taiwanensis Chen & Weng, 1967: 81, fig. 61 (Type locality: Tungkang, southwestern Taiwan). Chen, 1969: 134; Shen, 1984 a: 114; Chen & Yu, 1986: 255; Shen et al., 1993: 115. Gavialiceps taiwanensis (Chen & Weng, 1967): Karmovskaya, 1994: 84; Smith in Randall & Lim, 2000: 586; Shao et al., 2008: 239; Ho et al., 2010: 25; Ho & Shao, 2011: 24, table 1; Lin et al., 2013: 167. Gavialiceps taeniola (not of Alcock, 1889): Shao et al., 2008: 239. Remarks. The assignment of this species to the Muraenesocidae is provisional. This is the most abundant eel species found in bycatch of midwater trawls in Taiwan.Published as part of Ho, Hsuan-Ching, Smith, David G., Mccosker, John E., Hibino, Yusuke, Loh, Kar-Hoe, Tighe, Kenneth A. & Shao, Kwang-Tsao, 2015, Annotated checklist of eels (orders Anguilliformes and Saccopharyngiformes) from Taiwan, pp. 140-189 in Zootaxa 4060 (1) on pages 150-151, DOI: 10.11646/zootaxa.4060.1.16, http://zenodo.org/record/24365
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