407 research outputs found
Etablierung eines Organoid-Modells des ErbB2-positiven Mammakarzinoms zur Evaluierung einer zielgerichteten Immuntherapie mit CAR-NK-Zellen
No full textVIII, 204 Seiten ; Illustrationen, Diagramm
Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein
No full textBackground: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes. Expression of enzymatically active GrB in recombinant form is a prerequisite for functional analysis and application of GrB for therapeutic purposes. Methods and Findings: We investigated the influence of bacterial maltose-binding protein (MBP) fused to GrB via a synthetic furin recognition motif on the expression of the MBP fusion protein also containing an N-terminal alpha-factor signal peptide in the yeast Pichia pastoris. MBP markedly enhanced the amount of GrB secreted into culture supernatant, which was not the case when GrB was fused to GST. MBP-GrB fusion protein was cleaved during secretion by an endogenous furin-like proteolytic activity in vivo, liberating enzymatically active GrB without the need of subsequent in vitro processing. Similar results were obtained upon expression of a recombinant fragment of the ErbB2/HER2 receptor protein or GST as MBP fusions. Conclusions: Our results demonstrate that combination of MBP as a solubility enhancer with specific in vivo cleavage augments secretion of processed and functionally active proteins from yeast. This strategy may be generally applicable to improve folding and increase yields of recombinant proteins
Mediated counselling in the concept of mediatisation by Winfried Schulz
No full textIn this article, the author draws attention to the phenomenon of mediatisation (meaning intensive process of compacting the network of connections between media and society). It is noted that the processes of mediatisation penetrate different areas of life (such as family, political, public life, etc.). The author points out the consequences of these processes in the construction of counselling.
The various processes of mediatisation in mediated counselling (counselling in mass media) are identified in the article. The starting point is the concept of Winfried Schulz. The author of the article draws particular attention to the four (sub) processes of social change in which the media play a key role. These are: extension, substitution, amalgamation and accommodation. Each of these processes is identified by the author in mediated counselling. Each of them has an impact on the construction of counselling.
The author focuses on such phenomena as: exit counselling beyond time and space, extended availability of tips, "suspended advice", replacing the direct interaction by mediated interaction and even by intermediate quasi-interaction, multiplication of different counselling deals in the next (new) means of mass communication, mixing different types of mediated counselling in a range of media, generating problems
Forgotten and Newly Discovered Author - Werner Bräunig
No full textDiese Abschlussarbeit befasst sich mit dem "vergessenen" Autor Werner Bräunig (1934-1976). Er wurde wieder im Jahre 2007 "entdeckt", als sein verbotener Roman "Rummelplatz" erschien. In den ersten zwei Kapiteln werden kurz die Geschichte und die Literatur der Deutschen Demokratischen Republik (DDR) beschrieben. In dem Hauptteil beschäftigt sich die Arbeit mit dem Leben und den Werken von Werner Bräunig, hauptsächlich mit dem Roman "Rummelplatz". In diesem Teil wird auch die Bräunigs Beteiligung an dem kulturpolitischen Programm der Sozialistischen Einheitspartei Deutschlands (SED) sog. "Bitterfelder Weg" erklärt. Am Ende der Arbeit wird die Zensur in der DDR erwähnt. Gerade wegen der Zensur konnte Bräunig sein Roman nicht veröffentlichen.This bachelor's thesis deals with a "forgotten" author Werner Bräunig (1934-1976). His previously forbidden novel "Rummelplatz" was not published until 2007 and thanks to that the author was "newly discovered". In the first two chapters, the history and literature of the German Democratic Republic (GDR) are briefly described. The thesis's main part is dealing with Werner Bräunig's life and work, primarily his novel "Rummelplatz". This part also clarifies Bräunig's participation in the political-cultural program Socialist Unity Party of Germany, so called "Bitterfeld way". At the end of the thesis mentions a censorship in the GDR, because of which Bräunig could not publish his novel.Tato závěrečná práce se zabývá „zapomenutým“ autorem Wernerem Bräunigem
(1934-1976). Jeho dříve zakázaný román „Rummelplatz“ vyšel až v roce 2007 a díky
tomu byl tento autor „znovu objeven“. V prvních dvou kapitolách jsou krátce popsány
dějiny a literatura Německé demokratické republiky (NDR). V hlavní části se práce
zabývá životem a tvorbou Wernera Bräuniga, především románem „Rummelplatz“.
V této části je také vysvětlena Bräunigova účast na kulturně-politickém programu
Sjednocené socialistické strany Německa tzv. „Bitterfeldské cesty“. V závěru práce je
zmínka o cenzuře v NDR, kvůli které nemohl Bräunig svůj román publikovat.Katedra cizích jazykůDokončená práce s úspěšnou obhajobo
More Notes by Coleman
No full textIn an article published in Medium Ævum in 1949, Neil Ker was the first to note the appearance of signed marginal notes in three eleventh-century manuscripts from Worcester. These notes carry a cryptic signature reading 'Coleman' when deciphered and identify the author as the man of that name who served as chancellor to St Wulfstan in 1089 and prior of the cell of Westbury-on-Trym in 1093. Coleman and Thomas, prior of Worcester, died in 1113, Viri probitatis eximiae', according to the chronicle of John of Worcester. This is, of course, the Coleman who authored the lost vernacular life of Wulfstan II, Bishop of Worcester (d. 1095), used by William of Malmesbury in writing his own Latin life of that saint. In addition to these three signed notes, Ker attributed further annotations in Latin and Old English in the same manuscripts to Coleman, as well as marginalia found in two other eleventh-century Worcester manuscripts. The corpus of marginalia attributable to Coleman was expanded by Elizabeth Mclntyre in 1978 and nearly forty years after Ker's article, William Stoneman published a follow-up, also in Medium Ævum, in which he identified a further signed Old English note by Coleman. More recently, yet another Latin rubric was added to the growing list of Colemanian addenda by Rodney Thomson. What follows constitutes a further substantial contribution to the collection of identifiable traces which this churchman left in no less than eight manuscripts during the late eleventh and early twelfth centuries.https://www.questia.com/library/p5466/medium-aevum/i4076224/vol-79-no-1-201
More Notes by Coleman
No full textIn an article published in Medium Ævum in 1949, Neil Ker was the first to note the appearance of signed marginal notes in three eleventh-century manuscripts from Worcester. These notes carry a cryptic signature reading 'Coleman' when deciphered and identify the author as the man of that name who served as chancellor to St Wulfstan in 1089 and prior of the cell of Westbury-on-Trym in 1093. Coleman and Thomas, prior of Worcester, died in 1113, Viri probitatis eximiae', according to the chronicle of John of Worcester. This is, of course, the Coleman who authored the lost vernacular life of Wulfstan II, Bishop of Worcester (d. 1095), used by William of Malmesbury in writing his own Latin life of that saint. In addition to these three signed notes, Ker attributed further annotations in Latin and Old English in the same manuscripts to Coleman, as well as marginalia found in two other eleventh-century Worcester manuscripts. The corpus of marginalia attributable to Coleman was expanded by Elizabeth Mclntyre in 1978 and nearly forty years after Ker's article, William Stoneman published a follow-up, also in Medium Ævum, in which he identified a further signed Old English note by Coleman. More recently, yet another Latin rubric was added to the growing list of Colemanian addenda by Rodney Thomson. What follows constitutes a further substantial contribution to the collection of identifiable traces which this churchman left in no less than eight manuscripts during the late eleventh and early twelfth centuries.https://www.questia.com/library/p5466/medium-aevum/i4076224/vol-79-no-1-201
Target Cell-specific DNA Transfer Mediated by a Chimeric Multidomain Protein: NOVEL NON-VIRAL GENE DELIVERY SYSTEM
No full text⚘ Semiotic encounters with John Deely ☀ Winfried Nöth
No full text<p>Feel welcome... and you will become wide awake once you notice how deep the insights gained from the interplay between two semiotic animals can grow.</p>
<p>This event, commented by Myrdene Anderson (Purdue University) and co-chaired by William Passarini and Robert Junqueira (Institute for Philosophical Studies, both), is part of the activities of the 2022 International Open Seminar on Semiotics: a Tribute to John Deely on the Fifth Anniversary of His Passing, cooperatively organized by the Institute for Philosophical Studies of the Faculty of Arts and Humanities of the University of Coimbra, the Lyceum Institute, the Deely Project, Saint Vincent College, the Iranian Society for Phenomenology at the Iranian Political Science Association, the International Association for Semiotics of Space and Time, the Institute for Scientific Information on Social Sciences of the Russian Academy of Sciences, the Semiotic Society of America, the American Maritain Association, the International Association for Semiotic Studies, the International Society for Biosemiotic Studies, the International Center for Semiotics and Intercultural Dialogue, Moscow State Academic University for the Humanities and the Mansarda Acesa with the support of the FCT - Foundation for Science and Technology, I.P., of the Ministry of Science, Technology and Higher Education of the Government of Portugal under the UID/FIL/00010/2020 project.</p>
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<p>Winfried Nöth, Professor of Linguistics and Semiotics and Director of the Interdisciplinary Center for Cultural Studies of the University of Kassel until 2009, Visiting Professor at the University of Wisconsin (1985-86) and Humboldt University Berlin (2014-15), has been Professor of Cognitive Semiotics at the Pontifical Catholic University of São Paulo since 2010. He is an Honorary Member of the International Association for Visual Semiotics and the Institute for Edusemiotic Studies. His research is on topics of general and applied semiotics, cognitive semiotics, and Charles S. Peirce. Among his book publications are Handbook of Semiotics (1990, in German 2000), Mediale Selbstreferenz (2008) and Semiotic Theory of Learning (2018, with A. Stables, et al.). Nöth has edited Origins of Semiosis (1994), Semiotics of the Media (1997), and Crisis of Representation (2003), amongst others. Together with Lucia Santaella, he is the author of Imagem: Comunicação, semiótica e mídia (4th ed. 2005), Comunicação e semiótica (2004), Estratégias semióticas da publicidade (2010), and Introdução à semiótica (2017).</p>
<p>***</p>
<p>Myrdene Anderson received her Ph.D. from Yale University in 1978 and joined the faculty at Purdue University in 1977. Dr. Anderson has engaged in ethnographic research in a variety of settings, ranging from community garden associations in the U.S.A. to the international and interdisciplinary movement of artificial life in biology, but she is best known for her fieldwork among Saami reindeer-breeders in Norwegian Lapland, which research commenced in 1971 and continues to date. She has published over 150 articles and chapters in a variety of venues on a plethora of topics, and has edited a number of volumes on human-alloanimal ethology, on ethnicity and identity, on semiotic modeling, on the cultural construction of trash, on mathematics education, and on violence. Since 1983, more than 50 international and transdisciplinary symposia have been organized by Anderson. She has also been active on editorial boards of publications and on executive boards of professional societies. She served as president of the Central States Anthropological Society in 1993, and as president of the Semiotic Society of America in 1996. In 2003, Anderson was on a Fulbright in Estonia, where she both engaged in research and instructed in semiotics; as a courtesy, she also offered a semiotics course at the University of Helsinki. She has taught anthropology, linguistics, and semiotics and is responsible for one undergraduate core course (ANTH 414/LING 498—Language and Culture) and two graduate core courses (ANTH 514/LING 598—Anthropological Linguistics, and ANTH 605/LING 689—Ethnographic Analysis).</p>
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<p>Homepage: https://www.uc.pt/fluc/uidief/act/io2s<br>
Auditorium: https://www.uc.pt/fluc/uidief/act/io2s/auditorium</p>
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<p>Technical support assured by Robert Junqueira.</p>
<p>The official graphic designer of the IO2S DEELY is Zahra Soltani Tehrani.</p>
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