897 research outputs found
jason-weirather/AlignQC: AlignQC 2.0.4
No full text<p>An across the board improvement on stability. Better cross-platform compatibility. Docker added. Now <code>-t</code> or <code>--no_transcriptome</code> has replace <code>-a</code>, and <code>-g</code> or <code>--genome</code> has replaced <code>-r</code> to reduce the ambiguity. Now by default <code>-t</code> takes a GTF format file. with optional <code>--gpd</code> flag to indicate GPD format.</p>
Spatial signatures identify immune escape via PD-1 as a defining feature of T-cell/histiocyte-rich large B-cell lymphoma
No full textAbstract T-cell/histiocyte-rich large B-cell lymphoma (TCRLBCL) is an aggressive variant of diffuse large B-cell lymphoma (DLBCL) characterized by rare malignant B cells within a robust but ineffective immune cell infiltrate. The mechanistic basis of immune escape in TCRLBCL is poorly defined and not targeted therapeutically. We performed a genetic and quantitative spatial analysis of the PD-1/PD-L1 pathway in a multi-institutional cohort of TCRLBCLs and found that malignant B cells harbored PD-L1/PD-L2 copy gain or amplification in 64% of cases, which was associated with increased PD-L1 expression (P = .0111). By directed and unsupervised spatial analyses of multiparametric cell phenotypic data within the tumor microenvironment, we found that TCRLBCL is characterized by tumor-immune “neighborhoods” in which malignant B cells are surrounded by exceptionally high numbers of PD-L1–expressing TAMs and PD-1+ T cells. Furthermore, unbiased clustering of spatially resolved immune signatures distinguished TCRLBCL from related subtypes of B-cell lymphoma, including classic Hodgkin lymphoma (cHL) and DLBCL-NOS. Finally, we observed clinical responses to PD-1 blockade in 3 of 5 patients with relapsed/refractory TCRLBCL who were enrolled in clinical trials for refractory hematologic malignancies (NCT03316573; NCT01953692), including 2 complete responses and 1 partial response. Taken together, these data implicate PD-1 signaling as an immune escape pathway in TCRLBCL and also support the potential utility of spatially resolved immune signatures to aid the diagnostic classification and immunotherapeutic prioritization of diverse tumor types
Cloud Computing Offers Cheap Solutions
No full textAuthor\u27s biography: Jason Anderson is the director of the Georgia Southern University Small Business Development Center. He can be reached at [email protected]
Relations between acoustic and articulatory measurements of /l/
No full textVariation in the production of English /l/ has received significant study. It has been characterized in terms of categorical allophones, in terms of acoustic properties, and in terms of articulatory timing. Using a parallel corpus of acoustic-articulatory data from two speakers of American English, this study looks at the relations between acoustic and articulatory measurements of /l/ across words in corpus of read speech. We find significant negative correlations between F1 and tongue tip height and significant positive correlations between F2 and tongue body retraction. Additionally, we find that the relative timing of tongue tip and tongue back gestures in our data are consistent with past work on positional variants of /l/
Constraint Therapy With Progressive Incorporation of Bimanual Therapy Significantly Improves Hand Function in Children With Unilateral Brain Injury
No full textAbstract
Date Presented 3/31/2017
This study examined the efficacy of modified constraint-induced movement therapy, with progressive introduction of bimanual therapy to improve hand functions in children with unilateral brain injury participating in an intensive occupational therapy program.
Primary Author and Speaker: Ka Lai Kelly Au
Contributing Authors: Julie L. Knitter, Susan Morrow-McGinty, Jason B. Carmel, Kathleen M. Friel</jats:p
Study of parasite kinetics with antileishmanial drugs using real-time quantitative PCR in Indian visceral leishmaniasis
No full textObjectives This study describes parasite kinetics in the blood of visceral leishmaniasis patients treated with liposomal amphotericin B (L-AmB) or a preformed fat emulsion of amphotericin B (ApL) using real-time quantitative PCR (qPCR).
Methods Forty-six patients were treated with a single dose (15 mg/kg of body weight) of either L-AmB (n = 13) or ApL (n = 33). qPCR was used to estimate parasite kinetics by detection of Leishmania donovani DNA using kinetoplast DNA-specific primers in peripheral blood samples using an absolute quantification method.
Results The mean parasite load decreased from baseline (day 0) values of 894.07 and 980.48 to 71.72 and 211.52 parasite genomes/mL at day 7 in L-AmB and ApL groups, respectively, and at day 30 these further declined to 8.30 and 133.98 parasite genomes/mL, respectively. At day 30 post-treatment evaluation, the decline in parasite load was significantly greater (P = 0.024) with L-AmB compared with ApL. Four of 33 patients in the ApL group failed treatment (1 primary failure and 3 relapses) with the presence of parasites, whereas all patients in the L-AmB group were cured at 6 month follow-up.
Conclusions qPCR can be a tool to measure parasite dynamics accurately and provide a marker to measure the efficacy of various drugs. It can be used as a test of cure, allowing us to do away with invasive and risky methods such as splenic or bone marrow aspiration
Development of a time-limited group for adolescents with a relative who has cancer
No full textThis study explores the emotional and psychological needs of a group of adolescents who have a relative diagnosed with cancer. When a family endures a diagnosis of cancer, the entire family can be profoundly affected, including the healthy children in the family. Relatives of children with cancer are at an increased risk for Post-Traumatic Stress Syndrome as well as other emotional disturbances (Woodgate, 2006). For the present study, five adolescents took part in a qualitative interview process, and were self-referred through the Relay for Life, Ocean County, NJ chapter of the American Cancer Society. The goal of the study was to operationalize and better define the experience of having a loved one diagnosed with cancer and then determine the components of an effective support group. It was hypothesized that if adolescents participate in the development of a social support group, it will be more successful in retaining participants in the future. A qualitative research design was used to: 1) gain a better understanding of the overall experience of having a relative diagnosed with cancer and 2) to identify the specific needs of the adolescents who participated in the qualitative interview. The results of this study illustrate the gravity of having a loved one diagnosed with cancer, especially during adolescence. The primary themes that arose in this study included: 1) a need for more information regarding cancer and its treatment; 2) a need for emotional support; and 3) a psychosocial component to address positive and negative coping mechanisms. With the increased demands of school and social pressures on adolescents, there is a corresponding need for groups to help adolescents understand what is occurring, and learn how to cope when a family member is diagnosed with cancer. The results of this study are intended to guide future research, improve the experience of having a relative diagnosed with cancer, and provide a model for the development of future adolescent social support groups.Psy. D.Includes bibliographical referencesby Jason Thomas Ruc
Voice Compression and Communications: Principles and Applications for Fixes and Wireless Channels
No full textUp-to-date, expert coverage of topics in wireless voice communications Voice communication is the most important facet of mobile radio service. Even when the predicted surge of wireless data and Internet services becomes a reality, voice will remain the most natural means of human communication. Voice Compression and Communications details issues in wireless voice communications and treats compression, channel coding, and wireless transmission as a joint subject. Part I covers background material, whereas Part II provides detailed information on both proprietary and standardized analysis-by-synthesis codecs, including the speech codecs of virtually all existing wireline-based and wireless systems. Parts III and IV discuss mainly research-based wideband, audio, as well as very low-rate schemes likely to find their way into future standards. Voice Compression and Communications describes fundamental concepts in a non-mathematical way early in the book for those with only a background knowledge of signal processing and communications. More advanced readers will find detailed discussions of theoretical principles, future concepts, and solutions to various specific wireless voice communications problems
DNA fusion gene vaccination mobilizes effective anti-leukemic cytotoxic T lymphocytes from a tolerized repertoire
No full textThe majority of known human tumor-associated antigens derive from non-mutated self proteins. T cell tolerance, essential to prevent autoimmunity, must therefore be cautiously circumvented to generate cytotoxic T cell responses against these targets. Our strategy uses DNA fusion vaccines to activate high levels of peptide-specific CTL. Key foreign sequences from tetanus toxin activate tolerance-breaking CD4+ T cell help. Candidate MHC class Ibinding tumor peptide sequences are fused to the C terminus for optimal processing and presentation. To model performance against a leukemia-associated antigen in a tolerized setting, we constructed a fusion vaccine encoding an immunodominant CTL epitopederived from Friend murine leukemia virus gag protein (FMuLVgag) and vaccinated tolerant FMuLVgag-transgenic (gag-Tg) mice. Vaccination with the construct induced epitopespecificIFN-c-producing CD8+ T cells in normal and gag-Tg mice. The frequency and avidity of activated cells were reduced in gag-Tg mice, and no autoimmune injury resulted. However, these CD8+ T cells did exhibit gag-specific cytotoxicity in vitro and in vivo. Also, epitope-specific CTL killed FBL-3 leukemia cells expressing endogenous FMuLVgag antigen and protected against leukemia challenge in vivo. These results demonstrate a simple strategy to engage anti-microbial T cell help to activate epitope-specific polyclonal CD8+ T cell responses from a residual tolerized repertoire
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Validating Volunteered Geographic Information: Can We Reliably Trace Visitors' Digital Footprints?
No full textJason L. Stienmetz is earning his PhD in Business Administration at Temple University’s Fox School of Business. He is also a research coordinator at the Eric Friedheim Tourism Institute at the University of Florida. His research interests include tourism metrics, big data analytics, and smart tourism.
Daniel R. Fesenmaier is a Professor in the Department of Tourism, Recreation and Sport Management, University of Florida and Director of the Eric Friedheim Tourism Institute. His main research and teaching interests focus on the use of information and the Internet in travel decisions, the use of information technology for tourism marketing and the development of knowledge-based systems for tourism marketing organizations. Dr. Fesenmaier is the author of a number of articles dealing with tourism marketing, advertising evaluation and information technology. He has co-edited five books and is co-founding editor of the international journal Tourism Analysis.Passport to Research (Visual Papers)The objective of this study is to evaluate the quality of Volunteered Geographic Information (VGI) data for the purpose of describing patterns of visitor movement within a tourism destination. Using a Southern United States tourism destination as a case study, this research quantifies visitor flow networks using both data mined from Instagram and data collected using a traditional online survey methodology and then conducts a series of statistical analyses to compare results. In doing so, this paper highlights the advantages of using VGI data for tourism research, but also draws attention to potential trappings. Keywords: Volunteered Geographic Information (VGI), Instagram, visitor network, digital footprin
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