5,869 research outputs found
Comparative analysis of Mycobacterium tuberculosis pe and ppe genes reveals high sequence variation and an apparent absence of selective constraints.
No full textContains fulltext :
110619.pdf (Publisher’s version ) (Open Access)Mycobacterium tuberculosis complex (MTBC) genomes contain 2 large gene families termed pe and ppe. The function of pe/ppe proteins remains enigmatic but studies suggest that they are secreted or cell surface associated and are involved in bacterial virulence. Previous studies have also shown that some pe/ppe genes are polymorphic, a finding that suggests involvement in antigenic variation. Using comparative sequence analysis of 18 publicly available MTBC whole genome sequences, we have performed alignments of 33 pe (excluding pe_pgrs) and 66 ppe genes in order to detect the frequency and nature of genetic variation. This work has been supplemented by whole gene sequencing of 14 pe/ppe (including 5 pe_pgrs) genes in a cohort of 40 diverse and well defined clinical isolates covering all the main lineages of the M. tuberculosis phylogenetic tree. We show that nsSNP's in pe (excluding pgrs) and ppe genes are 3.0 and 3.3 times higher than in non-pe/ppe genes respectively and that numerous other mutation types are also present at a high frequency. It has previously been shown that non-pe/ppe M. tuberculosis genes display a remarkably low level of purifying selection. Here, we also show that compared to these genes those of the pe/ppe families show a further reduction of selection pressure that suggests neutral evolution. This is inconsistent with the positive selection pressure of "classical" antigenic variation. Finally, by analyzing such a large number of genes we were able to detect large differences in mutation type and frequency between both individual genes and gene sub-families. The high variation rates and absence of selective constraints provides valuable insights into potential pe/ppe function. Since pe/ppe proteins are highly antigenic and have been studied as potential vaccine components these results should also prove informative for aspects of M. tuberculosis vaccine design
Diseño y gestión del E-commerce para la psicóloga Rachel Watson Delucchi
No full textThe present project is about the design and management of e-commerce for the clinical
and educational psychologist Rachel Watson Delucchi. This proposal was created during
the pandemic caused by COVID-19, with the purpose of providing socio-emotional
support to Peruvians who were experiencing difficulties due to the situation. A website
was designed and executed in a professional manner to bring a new platform for her
services and products, so that her patients and followers can choose the best alternative
for their personal, family or business counseling and support.El presente proyecto consiste en la creación del diseño y gestión del e-commerce para la psicóloga clínica y educacional Rachel Watson Delucchi. Esta propuesta nace durante la pandemia ocasionada por el COVID-19, con la finalidad de brindar apoyo socioemocional a los peruanos que estuvieran atravesando dificultades por la coyuntura. Se diseñó y desarrolló un portal digital web para ofrecer sus servicios y productos de manera profesional, para que sus pacientes y seguidores puedan escoger la mejor alternativa de apoyo para su persona, familia o empresa
The Client's and Therapist's Vocal Qualities in CBT and PE-EFT for Depression
No full textThe psychotherapy client’s vocal quality indicates the quality of and shifts in the client’s engagement in treatment. In contrast, the therapist’s vocal quality is a treatment intervention, either facilitating or hindering the client’s progress. The Client Vocal Quality (CVQ) and Therapist Vocal Quality (TVQ) measures (Rice & Kerr, 1986) were applied to responses in the middle 20 minutes of low and high change sessions for 61 clients who received either cognitive behavioural therapy (CBT) or process-experiential emotion focused therapy (PE-EFT) for depression (Watson, Gordon, Stermac, Kalogerakos, & Steckley, 2003). Sessions were selected using the Client Task Specific Change Measure-Revised (CTSC-R; Watson, Greenberg, Rice, & Gordon, 1996). Outcome measures included the Beck Depression Inventory, Dysfunctional Attitude Scale, Global Severity Index of the Symptom Checklist-90-Revised, Inventory of Interpersonal Problems, Problem-Focused Style of Coping Scale, and Rosenberg Self-Esteem Scale. The CVQ categories differentiated between treatment types, with PE-EFT clients expressing a significantly higher proportion of Emotional vocal quality and CBT clients expressing a significantly higher proportion of Externalizing vocal quality. There was no difference between the treatment types for Focused or Limited vocal quality. The CVQ categories predicted outcome. This relationship was particularly evident in the session in which clients first reported moderate to high change on the CTSC-R. The combination of Emotional and Focused categories was a stronger predictor than either category alone. In contrast, a higher proportion of Externalizing vocal quality predicted worse scores. In terms of Therapist Vocal Style, PE-EFT therapists spoke predominantly in the Softened-Irregular Vocal Style, while CBT therapists spoke predominantly in the Natural-Definite Vocal Style. Therapists’ use of a Softened-Irregular Vocal Style, compared with a Natural-Definite Vocal Style, was associated with the client’s report, at the end of therapy, that he/she felt less easily exploited by others and had a greater ability to assert interpersonal boundaries without fear of offending others. Limitations of the study are discussed.Ph
The Client's and Therapist's Vocal Qualities in CBT and PE-EFT for Depression
No full textThe psychotherapy client’s vocal quality indicates the quality of and shifts in the client’s engagement in treatment. In contrast, the therapist’s vocal quality is a treatment intervention, either facilitating or hindering the client’s progress. The Client Vocal Quality (CVQ) and Therapist Vocal Quality (TVQ) measures (Rice & Kerr, 1986) were applied to responses in the middle 20 minutes of low and high change sessions for 61 clients who received either cognitive behavioural therapy (CBT) or process-experiential emotion focused therapy (PE-EFT) for depression (Watson, Gordon, Stermac, Kalogerakos, & Steckley, 2003). Sessions were selected using the Client Task Specific Change Measure-Revised (CTSC-R; Watson, Greenberg, Rice, & Gordon, 1996). Outcome measures included the Beck Depression Inventory, Dysfunctional Attitude Scale, Global Severity Index of the Symptom Checklist-90-Revised, Inventory of Interpersonal Problems, Problem-Focused Style of Coping Scale, and Rosenberg Self-Esteem Scale. The CVQ categories differentiated between treatment types, with PE-EFT clients expressing a significantly higher proportion of Emotional vocal quality and CBT clients expressing a significantly higher proportion of Externalizing vocal quality. There was no difference between the treatment types for Focused or Limited vocal quality. The CVQ categories predicted outcome. This relationship was particularly evident in the session in which clients first reported moderate to high change on the CTSC-R. The combination of Emotional and Focused categories was a stronger predictor than either category alone. In contrast, a higher proportion of Externalizing vocal quality predicted worse scores. In terms of Therapist Vocal Style, PE-EFT therapists spoke predominantly in the Softened-Irregular Vocal Style, while CBT therapists spoke predominantly in the Natural-Definite Vocal Style. Therapists’ use of a Softened-Irregular Vocal Style, compared with a Natural-Definite Vocal Style, was associated with the client’s report, at the end of therapy, that he/she felt less easily exploited by others and had a greater ability to assert interpersonal boundaries without fear of offending others. Limitations of the study are discussed.Ph
Estudio Hipoglicemiante de Amaranthus powelli S. Watson “atajo”
No full textObjetivo: Determinar la acción hipoglicemiante de Amaranthus powelli S. Watson “atajo” a partir del extracto acuoso, de las hojas en animales de experimentación. Lugar; Facultades: Ciencias Biológicas (Laboratorio de Farmacognosia), Medicina Humana (Laboratorio de Farmacología) y Farmacia y Bioquímica (Laboratorio de Cemprofarma) de la UNMSM. Material y Método: se emplearon 60 ratones machos albinos Mus musculus de la cepa HSDMIH y 84 ratas machos albinas de la especie Rattus novergicus cepa Holtzmann. Método empleado CYTED 1995.
Resultados: Taxonómicamente la planta conocida como “atajo” es Amaranthus powelli S. Watson. La DL50 es de 8831,45 mg/Kg. para Amaranthus powelli S. Watson, se observa una reducción de glicemia en ratas sometidas a la prueba oral de glucosa, siendo mayor en los animales que recibieron 100 mg/kg. La planta no es tóxica. Se identificaron los principios activos responsables de la actividad farmacológica, los cuales se determinaron como metabolitos secundarios, el estudio fitoquímico del extracto metanólico y etéreo indican la presencia de taninos, flavonoides, esteroides, triterpenoides y alcaloides. La existencia de cromo y magnesio, se determinó en el análisis de minerales, 2 minerales importantes, el cromo en el metabolismo de la glucosa y el magnesio en la secreción de insulina. El estudio histopatológico en el hígado, bazo y riñones de las ratas demostró que la planta no produce lesión en ellas. Conclusión, el extracto acuoso de las hojas de Amaranthus powelli S.Watson baja el nivel de glucosa, siendo mayor en los animales que recibieron 100 mg/Kg. de extracto.Tesi
On minimal non-PE-groups
No full textAbstractIf H is a subgroup of a finite group G, by HG denote the normal closure of H in G. G is called a PE-group if every minimal subgroup X of G satisfies NG(X) ∩ XG = X. The author proves that all PE-groups are solvable with the Fitting height at most 3 and classifies the minimal non-PE-groups
Filellum nitidum Watson 2005
No full text<i>Filellum nitidum</i> Watson, 2005 <p> <i>Filellum nitidum</i> Watson, 2005: 504 –505, figs. 3c–e.</p> <p> <b>Type series.</b> Holotype— <i>Filellum nitidum</i> Watson, 2005, fertile colony on stem of <i>Gymnangium</i> sp. (NMV F 101650). Paratype— <i>Filellum nitidum</i> Watson, 2005, fertile colony on basal stems of additional colonies of <i>Gymnangium</i> sp. (WAM Z29955) (Watson 2005: 504).</p> <p> <b>Type locality.</b> From the holotype, Australia, Middle Island, 25 m (Watson 2005: 504).</p> <p> <b>Description.</b> See Watson (2005: 504).</p> <p> <b>Distribution.</b> <i>Filellum nitidum</i> is only known from its original description, from Australia, Middle Island (Watson 2005).</p> <p> <b>Remarks.</b> <i>Filellum nitidum</i> was described by Watson (2005: 504) as bearing “Hydrothecae sessile […] adnate part of base fairly short, free part long, perisarc of abcauline wall faintly wrinkled; perisarc of free wall rather thick, marginal rim slightly everted and thickened” and “hydrothecae with several regenerations along length”. Surprisingly, in spite of the original description and the figure 3C, where wrinkled hydrothecae are shown, Watson (2005: 505) also stated that the “absence of marked abcauline rugosity and absence of a distinctly everted rim” would make the trophosome of the species similar to that of <i>F. antarcticum</i>. This seems to be an inapt description, since <i>F. antarcticum</i> has distinctly marked striae on the upper side of the adnate portion of the hydrothecae (Peña Cantero <i>et al.</i> 2004b). On the other hand, as also remarked by Watson (2005), both species have clearly different gonothecae. Other morphological features listed by Watson (2005) do not distinguish this species from the others of the genus <i>Filellum</i>. Therefore, we believe that reliable diagnosis of <i>F. nitidum</i> must be based on coppiniae characters, described as “surface of coppinia a mosaic covered by a thin pellicle of tissue perforated by small openings flush with surface; hydrothecae protruding above surface together with large inverted funnel-shaped tubules, some tubules bifurcated, apices of others closed” and the sectional view of “numerous rather haphazardly arranged elongate gonothecae” (Watson 2005: 504–505). The figure illustrating the coppinia of this species (Watson 2005: 503, figs. 3D–E) shows protective tubes among the gonothecae; this, together with the different shape of the gonothecae, make the species distinct from <i>F. magnificum</i>, for which the defensive tubes are mainly situated on the periphery of the mass of gonothecae, like a fence, arching over the gonothecae. There is no information on the cnidome of the species, and no further comparison concerning this character can be made.</p>Published as part of <i>Marques, Antonio C., Peña, Álvaro L., Miranda, Thaís P. & Migotto, Alvaro E., 2011, Revision of the genus Filellum Hincks, 1868 (Lafoeidae, Leptothecata, Hydrozoa), pp. 1-28 in Zootaxa 3129</i> on page 15, DOI: <a href="http://zenodo.org/record/206783">10.5281/zenodo.206783</a>
Cuidado perioperatorio de enfermería según Jean Watson en un instituto de salud de Lima 2019
No full textObjetivo: Determinar el cuidado perioperatorio según Jean Watson en un Instituto de salud de Lima 2019. Material y Método: El nivel de la investigación es de tipo cuantitativo, de corte transversal y con diseño descriptivo, toda vez que se detalla el cuidado perioperatorio según la teoría de Watson que brindan las enfermeras de centro quirúrgico a pacientes con patología cardiaca. El estudio se realizará en el Instituto Nacional Cardiovascular INCOR, con una muestra conformada por 20 enfermeras especialistas del servicio de Centro Quirúrgico de esta institución. La técnica que se aplicará para la recolección de datos del presente estudio será la observación y el instrumento será una guía de observación, a través de la escala en la medición del cuidado humano transpersonal basado en la Teoría de J. Watson, que consta de 38 ítems dividido en 3 dimensiones: interacción enfermera paciente, campo fenomenológico y necesidades humanas, lo cual permitirá aplicar la estadística descriptiva
[[alternative]]The Study of Acknowledgement of Risk Management on Perception of PE Teachers in Taoyuan Junior High Schools
No full text[[abstract]]The purpose of this study was to (1) explore the acknowledgement about physical activity risk management of junior high school PE teachers in Taoyuan, (2) compare PE teachers’ perception difference about risk management with different background (3) realize the strategy of risk management plan of PE teachers in junior high school. (4) finally build effective risk management plan for all PE teachers in junior high school.
One hundred and ninety two Taoyuan PE teachers served as the subjects of this study with the questionnaire of ” PE Teacher’s Perception of The Risk Management”. This study used descriptive statistics, t-test, and one-way ANOVA to analyze data. Besides, the research also chose seven PE teachers to do semi-structured interview, trying to understand risk management strategy of PE teachers. The results were listed as below:
1.The perception of risk management of physical teachers in junior high school, based on the importance, was: theory layer, practical layer, law layer, entity and application layer.
2.Significant difference was found in gender, practical layer, law layer and entity; while other factors showed no significant difference.
3.All the interviewees didn’t take action about risk management , but all of them agreed that risk management plan of physical activity is necessary. They suggested higher authorities ask junior high school to set risk management plan for physical activity, and become an evaluated item.
In conclusion of the research, the PE teachers in junior high school think it is important to execute a better physical activity risk management. Author suggested junior high school should set risk management plan for physical activity to get “zero risk” in school.
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