18,607 research outputs found
Pharmaceutical chemistry / edited by David G. Watson.
No full textpharmacy bookfair2015Includes bibliographical references and index.ix, 641 p.
Non-maximal cyclic group actions on compact Riemann surfaces
No full textWe say that a finite group G of automorphisms of a Riemann surface X is non-maximal in genus y if (i) G acta as a group of automorphisms of some compact Riemann surface Xg of genus g and (ii), for alí such surfaces Xg, | Aut Xg |>| G |. In this paper we investigate ihe case where G is a cylic group Cn of order n. If Cn acts on only finitely many surfaces of genus g, then we completely solve the problem of finding all such pairs (n, g)
Surgical antisepsis and the risk of operating theatre fires
No full textMatthias Maiwald, Chris J. M. Farmer, David G. Lance, Vincent B. Nieuwenhuijs, Christopher H. Heath, David I. Watson, David L. Gordo
Data for 'A model for stochastic prediction of track support stiffness'
No full textThis dataset supports the publication:
Le Pen, L., Milne, D., Watson, G., Harkness, J., & Powrie, W. (Accepted/In press). A model for stochastic prediction of track support stiffness. Proceedings of the Institution of Mechanical Engineers, Part F: Journal of Rail and Rapid Transit.
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The effect of oxidation on the stability of G-quadruplex DNA : implications for oncogene expression
No full textG-quadruplexes (G4-DNA) are a class of secondary structures formed from Guanine rich sequences. In recent years these structures have been implicated in both telomere maintenance and oncogene expression, and have been shown to be abundant in upstream promoter regions and at telomeric ends.
The mutagenic properties of oxidative stress on DNA have been widely studied, as has the association with carcinogenesis. The oxidation of deoxyguanosine to 8-oxo-2’deoxyguanosine (8-oxo-dG) is the most common result when DNA is under oxidative stress and as such, the G-rich sequences that form G-quadruplexes can be viewed as potential “hot-spots” for DNA oxidation. We propose that oxidation may destabilise the G-quadruplex structure, leading to its unfolding into the duplex structure, affecting gene expression. This would imply a possible mechanism by which oxidation may impact on oncogene expression.
This project used both in silico and in vitro methods to observe the effect of oxidation on the G-quadruplex structure and the consequences in oncogene expression, using two biologically relevant G-quadruplex structures, those found in the promoter regions of the proto-oncogenes c-Myc and c-Kit as proof of concept.
Molecular dynamics (MD) simulations were performed (isothermic, isobaric 500ns unrestrained simulation in explicit solvent and counterions) on the c-Kit and c-Myc G-quadruplex structures with and without 8-oxo-dG incorporated into the central tetrad. FRET experiments were performed on these same structures, observing the conformation of sequences known to form G-quadruplexes under near physiological conditions and subjected to oxidative stress, through Fenton chemistry. Gene expression data analyses were also performed to evaluate the prevalence of different G-quadruplex forming motifs (GQMs) in genes affected by oxidation.Although no relevant information was gained from the FRET experiments, the MD results constitute the longest simulations of this type performed on the c-Myc and c-Kit G-quadruplex structures published to date and predict the high stability of these structures under normal physiological conditions. They also clearly demonstrate a destabilising effect of oxidation on G-quadruplex structures, with the extent of the effect dependent on the structure oxidised.
Furthermore, gene expression data analysis showed that genes whose expression is significantly altered when subjected to oxidative stress are statisticallymore likely to contain a GQM than the remainder of the genome, through the use of significance testing.
These findings demonstrate a differential effect of oxidation on G-quadruplexes, likely dependent on other known characteristics affecting G4 stability such as loop length and sequence. Results also point towards this mechanism affecting gene expression. This is suggestive of a novel route for oxidation mediated carcinogenesis, through upregulation of oncogene expression or possibly downregulation of tumour suppression genes
Pharmaceutical analysis : a textbook for pharmacy students and pharmaceutical chemists / David G. Watson ; with a contribution by RuAngelie Edrada-Ebel.
No full textpharmacy bookfair2016Includes bibliographical references and index.xi, 427 pages :This introductory text highlights the most important aspects of a wide range of techniques used in the control of the quality of pharmaceuticals. Written with the needs of the student in mind, this clear, practical guide includes self-testing sections with arithmetical examples and tests to help students brush up on their arithmetical skills in an applied context
sj-docx-1-pss-10.1177_09567976211007463 – Supplemental material for Who Are You? The Study of Personality in Patients With Anterograde Amnesia
No full textSupplemental material, sj-docx-1-pss-10.1177_09567976211007463 for Who Are You? The Study of Personality in Patients With Anterograde Amnesia by McKenna M. Garland, Jatin G. Vaidya, Daniel Tranel, David Watson and Justin S. Feinstein in Psychological Science</p
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