1,120 research outputs found
Four-wave mixing of gold nanoparticles for three-dimensional cell microscopy
No full textOptical microscopy is an indispensable tool for obtaining spatial and temporal resolution within living cells and tissues. In this paper, a novel multiphoton microscopy technique which exploits the third-order nonlinearity called four-wave mixing and applied to gold nanoparticles in resonance with their surface plasmon is developed. The coherent, transient and resonant nature of this signal allows the interferometric time-resolved detection of four-wave mixing specifically originating from GNPs, with particle diameters down to 5nm. This paper demonstrate high-contrast, background-free imaging of gold-labelled Golgi structures in HepG2 cells with a sub-diffraction-limit lateral (axial) resolution of 140nm (470nm) at excitation powers corresponding to less than 3K photothermal heating, compatible to live cell imaging. These results pave the way towards photostable non-toxic and highly sensitive in-vivo optical imaging with sub-micron three-dimensional resolution
For Peter
No full textPart of the Yangai Broadside IV Series.Signed by the author and illustrator. Numbered G / 30
Hugh Dalziel Duncan: the forgotten interactionist?
No full textThe symbolic interactionist tradition is alive and well as measured by the number of researchers, publications and associational bodies, identifying with this approach. Yet the history of symbolic interactionism and its debt to early Chicago sociology is the source of some debate. In this paper, I examine the writings of a post-war adherent of this perspective who is little discussed these days: Hugh Dalziel Duncan. In a series of books, the most influential of which would be Communication and Social Order, this author sought to offer an alternative to the kind of 'mechanistic' models prevalent in American sociology of during the 1950s and 60s. Relying on the theories of Dewey, Mead, Simmel and Burke, he also took fellow symbolic interactionists to task for their failure to explain how symbols do their work or what it means to describe interaction as the performance of 'agency'. Pre-empting the recent cultural turn, Duncan made no distinction between the humanities and social sciences, and sought to place the study of aesthetic forms such as comedy at the centre of sociological reflection. Why then are his many publications so little read today
Narrative Rhetoric in Representing the British Aristocracy: Julian Fellowes and Peter Morgan
No full textThe essay aims at analyzing the different narrative and rhetorical approaches of two prominent British screenwriters to the representation of British aristocracy. The works by Julian Fellowes and by Peter Morgan offer the recent most popular contributions to the representation of British aristocratic life-style and values in contemporary mainstream culture. Besides the global success of their stories, a reason of interest for going deep into the way the two writers tackle the topic resides in their different political and ideological perspectives. On the one side, Fellowes’s depiction of aristocracy in his hit prime time TV drama Downton Abbey (ITV, 2010-2015) draws on the open conservative mindset of the author (“The BBC wouldn’t have done that... They are not happy with dramas that do not reflect their own political and philosophical viewpoint”). On the other side, portraying the Royal Family first in the film The Queen (2006), then in the TV series The Crown (Netflix, 2016), Morgan’s believes – opposite to Fellowes’ ones – were spurred (“it’s absolutely scandalous that they should still exist in an egalitarian society”). The aim of my analysis is twofold. First, to highlight how both Fellowes and Morgan had to control and balance the expression of their ideological credo so that their narrative wouldn’t be felt as “preachy”. Second, to identify which thematic solutions allowed them to make aristocracy (a topic generally felt as remote and distant) engaging for contemporary audiences
Planning for a future of asset-based welfare? New Labour, financialized economic agency and the housing market
No full textThis article focuses on core aspects of the political economy of New Labour and surveys the strategic priorities to which it is likely the planning process will have to adapt. As with other policy areas, the effects of enhanced Treasury micro-management of the Government's reform agenda has begun to impact upon the field of planning. The prime example in this respect is the Treasury's preference for replacing state provision of welfare-enhancing services with the move towards an individualized system of asset-based welfare. The article begins with an analysis of this shift, showing how it is dependent on creating financialized economic agents who think instinctively as active saver-investors in their quest to accumulate assets to fund future consumption of welfare. In contemporary Britain the housing market dominates the accumulation of assets amongst everyday saver-investors. The article concludes by analysing the possible tension that will be introduced into the planning process because of New Labour's twin goals: (1) to defend the current value of asset wealth even as the mortgage lending market has stalled and confidence in the stability of house prices has temporarily evaporated; and (2) to restrict exclusion from private ownership in the housing market so that broadening access can be used to propel a universal move towards an individualized system of asset-based welfare. The fallout from the world credit crunch, which began in autumn 2007 and remains ongoing at the time of writing in January 2009, looks likely to exacerbate what was always a tension-prone combination of objectives
The effective incorporation of research in undergraduate econometrics
No full textThis chapter examines the integration of research into undergraduate econometrics teaching through a focus on replication and reproduction (R&R) of published studies. Rather than limiting students to the passive reception of research findings, the R&R approach fosters active engagement by requiring them to work directly with real data and methods in order to reproduce results and interrogate techniques. The discussion situates R&R within broader debates on the role of research in teaching, highlighting its potential to make scholarship more accessible and relevant to students. Practical case studies and classroom-ready materials from the Economics Network Ideas Bank are presented, illustrating how this approach can enhance learning, develop quantitative skills, and build student confidence. Finally, the chapter shows how R&R aligns with wider educational goals, including employability, self-efficacy, and sustained engagement
The christology of Alexander of Hales
No full textThis thesis is a study in the Christology of Alexander of Hales. I chose Alexander as a subject simply because I wanted to become acquainted with a theology not of my own tradition and there is no better period to choose for this purpose than the thirteenth century, the century of the scholastic giants. Having chosen the period the next question to be asked was "who could I study besides Thomas Aquinas"? The fact that there were good new texts available of Alexander's main works and the fact that he was a man of some stature in his own day together with the fact that most Protestants have never heard of him seemed a good reason to discover the source of his great reputation. There were problems about the text of the Summa in that there were doubts as to its genuineness but I hoped that these problems would be solved as I proceeded.
However, this was not to be so. Because of the
doubts about the genuineness of the Summa this thesis has
become a study in the Christology of Alexander which appears
in the Glossa on the Sentences of Peter Lombard. Together
with this there are discussions of the views of the
Quaestiones at the end of each topic in this thesis
Genetic basis of congenital myeloid failure syndromes in mutant zebrafish
No full textDeposited with permission of the author. © Dr. Duncan Peter CarradiceZinc finger and BTB domain containing proteins (BTB-ZF) are transcriptional repressors from a family including members with critical roles in haematopoiesis and oncogenesis. From an N-ethyl-N-nitrosourea (ENU) mutagenesis screen for defects in myeloid development, a zebrafish mutant deficient in cells expressing myeloperoxidase (mpx) designated marsanne (man) was identified. Positional cloning identified that man carried a mutation in zbtb11, a largely unstudied BTB-ZF transcription factor, suggesting that zbtb11 is critical for normal neutrophil development.
The mutant man was found in a gynogenetic haploid ENU screen for defective expression of genes along the developmental pathway from mesoderm to mature neutrophil, undertaken to search for novel genetic regulators of myelopoiesis in an unbiased fashion. Since zebrafish are ectothermic, embryos were screened at 33°C to maximise recovery of temperature dependant alleles; man was the single temperature dependent mutant recovered.
man was a recessive, early embryonic lethal mutant with normal expression ofgenes involved in early haematopoietic differentiation and specification but markedly reduced expression of mpx, a gene expressed in terminally differentiated neutrophils. Erythropoiesis was unaffected. man mutants also developed brain and spinal cord degeneration with hydrocephalus, with marked apoptosis throughout the central nervous system.
Positional cloning resolved the genetic interval containing the man mutation to52.5 Kb containing the open reading frame of a single gene, zbtb11. Sequencingidentified a putative missense mutation at nucleotide 346 (T>A), causing aC116S substitution. Genetic tests implicated this zbtb11 mutation as the cause of the man phenotype. Antisense morpholino oligonucleotide knockdown of zbtb11 recapitulated the mpx deficiency and other features of the man phenotype. Injection of WT but not mutant zbtb11 mRNA into man embryos completely rescued expression of mpx and all other aspects of the man phenotype. Human ZBTB11 mRNA also rescued man mutants, indicating a wide evolutionary conservation of Zbtb11 function.
Utilising the rescue of man as an in vivo bioassay for Zbtb11 function, truncated and mutated forms of Zbtb11 were tested for functionality. The C terminal zinc fingers were not required for Zbtb11 function in this assay. The function of the N terminal domain of the protein, containing the residue mutated in man, was also explored.
These studies demonstrate a function for Zbtb11 in vertebrate neutrophil development. Ongoing study of the man mutant will provide further insights into Zbtb11 function and define its exact role in haematopoiesis
Trends of Europeanization in social welfare politics. IHS Political Science Series 82, July 2002
No full textFritz W. Scharpf (2000 and 2002) defines the term Europeanization as the progressive shift of governmental tasks to the European level. According to this understanding he identifies four modes of Europeanization. Further, he recognizes the establishment of minimum standards and the open method of co-ordination as specific modes of Europeanization. This paper first relates the welfare political goals and problems of both named methods of Europeanization in social welfare politics, then describes the political processes which accompany them, and subsequently tests whether Scharpf’s analysis can be affirmed
Obstacles to International Action Against State Terror in the Post-Cold War International System
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