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ฤทธิ์การยั้บยั้งแบคทีเรีย Streptococcus pneumoniae ของโรโดไมรโทน โดยการวิเคราะห์ทางโปรติโอมิกส์และเมตาโบโลมิกส์
No full textThesis (Ph.D., Microbiology)--Prince of Songkla University, 2019The emergence of antibiotic-resistant pathogenic bacteria is a healthcare problem worldwide. We evaluated the antimicrobial activity of rhodomyrtone, an acylphloroglucinol present in Rhodomyrtus tomentosa leaves, against the human Gram- positive pathogens, Streptococcus pneumoniae. The compound exhibited pronounced anti-pneumococcal activity against a broad collection of clinical isolates. We studied the effects at the molecular level by a combination of proteomics and metabolomics. The integration of proteomic and metabolomic analyses revealed alterations in enzymes and metabolites involved in different metabolic pathways including amino acid biosynthesis, nucleic acid biosynthesis, glucid, and lipid metabolism. Notably, the levels of two enzymes (glycosyltransferase and UTP-glucose-1-phosphate uridylyltransferase) and three metabolites (UDP-glucose, UDP-glucuronic acid and UDP-N-acetyl-D-galactosamine) participating in the synthesis of the pneumococcal capsule clearly diminished in cells exposed to rhodomyrtone. Rhodomyrtone-treated pneumococcal cells significantly possessed less amount of capsule, as measured by a colorimetric assay and visualized by electron microscopy. These findings reveal the utility of combining proteomic and metabolomic analyses to provide insight into phenotypic features of S. pneumoniae treated with this potential novel antibiotic.
The ability of Rhodomyrtus tomentosa leaf extract and rhodomyrtone to prevent biofilm formation and eradicate mature biofilms was assessed. The extract and rhodomyrtone at 1/8 × MIC significantly inhibited biofilm formation in all clinical isolates (P < 0.05). The viability of 8-day biofilm-grown cells significantly decreased following the treatment with the extract and rhodomyrtone at 16 × MIC. 40-90% reduction in the bacterial adhesion and invasion to A549 human alveolar epithelial cells was observed after challenging with the extract and rhodomyrtone, compared with the control within 60 min. Increase in 90-99% phagocytosis of the bacterial cells by RAW264.7 macrophage cell line was detected following the treatment with the extract and rhodomyrtone at 1/2 × MIC, compared with the control. The results suggested potential medicinal benefits of the extract and rhodomyrtone for the treatment of pneumococcal infections.
In conclusion, rhodomyrtone is a promising alternative antibacterial agent for the treatment of the infections caused by the Gram-positive pathogens including S. pneumoniae.อุบัติการณ์เชื้อแบคทีเรียดื้อยาปฏิชีวนะเป็นปัญหาสําคัญทั่วโลก งานวิจัยนี้ศึกษาฤทธิ์ของสาร
โรโตไทร โทนซึ่งเป็นกลุ่ม acyphloroglucinol แยกจากใบปกระทุในการต้านเชื้อแบคทีเรียก่อโรคกรัมบวก Streptococcus pneumoniae สารโร โตมร โทนมีฤทธิ์ต้านเชื้อแบคทีเรีย S. poweramoniae ที่แยกได้จากผู้ป่วย ศึกษา ฤทธิ์ ของโรโดไมร โทนในระดับโมเลกุลโดยวิธีโปรตีโอมิกส์และเมตาโบโลนิกส์ พบว่ามีการเปลี่ยนแปลงของ
เอนไซม์และสารเมทาบอไลท์ที่เกี่ยวข้องกับกระบวนการเมทาบอลิซึมของการสังเคราะห์กรดอะมิโน กรด นิวคลีอิก คาร์โบไฮเดรต และไขมัน ของเชื้อ S. wemonine ในสภาวะที่มีสารโรโตไมร โทน เป็นที่น่าสังเกตว่า ระดับของเอนไซม์ glycosyltransferase และ UTP-glucose-1-phosphate uridylyltransferase และสารเมทาบอไลท์ ประกอบด้วย UDP-glucose, UDP-glucuronic acid และ UDP-N-acetyl-D-galactosamine ลดลงในสภาวะที่มีสาร โรโตไทร โทน โดยเอนไซม์และสารดังกล่าวเกี่ยวข้องกับกระบวนการสังเคราะห์แคปซูลของเชื้อ S. pneumoniae สารโรโตไทร โทนลดการสังเคราะห์แคปซูลของเชื้อ S. pneumoniae ซึ่งศึกษาด้วยการวัดปริมาณแคปซูลและ
ภาพถ่ายอิเล็กตรอนชนิดส่องผ่าน
สารสกัดใบกระทที่สกัดด้วยเอาทนอก ด้วยเอาทนอล และสารบริสุทธิ์โรโตไทร โทนที่ความเข้มข้น 1/18 x MIC ยับยั้งการสร้างไบโอฟิล์มของเชื้อ S. pneumoniae ที่แยกได้จากผู้ป่วย อย่างมีนัยสําคัญ สารสกัดใบกระทุและ โรโตไมร โทนที่ความเข้มข้น 16 × MIC ฆ่าเชื้อในไบโอฟิล์มที่มีอายุ 8 วัน อย่างมีนัยสําคัญ สารสกัดในกระทู้และ โรโตไทร โทนลดการเกาะติดและการบุกรุกของเชื้อ S. Jesusmoine ต่อ 2549 human alveolar epithelial cells ภายในเวลา 60 นาที เชื้อ S. pneumoniae ที่เลี้ยงในสภาวะที่มีสารสกัดใบกระทุและโร โตมร โทนถูกจับกินโดย RAW264.7 macrophage cell line เพิ่มขึ้น 90-99% เมื่อเทียบกับชุดควบคุม จากผลการทดลองที่กล่าวมาแสดงให้ เห็นถึงศักยภาพของสารโรโดไมร โทนในการยับยั้งเชื้อ S. pneumoniaer
โดยสรุป rhodomyrione เป็นสารต้านแบคทีเรียทางเลือกที่มีแนวโน้มสําหรับการรักษาโรคติด
เชื้อที่เกิดจากเชื้อกรัมบวกที่มีสาเหตุจากเชื้อ S. pneumonia
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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