100,512 research outputs found
The rare mantis shrimp Areosquilla indica (Hansen, 1976) (Crustacea, Stomatopoda) from the Great Barrier Reef: first Australian records of the genus and species
No full textAhyong, Shane T., Wassenberg, Theodore J. (2015): The rare mantis shrimp Areosquilla indica (Hansen, 1976) (Crustacea, Stomatopoda) from the Great Barrier Reef: first Australian records of the genus and species. Zootaxa 4000 (4): 492-496, DOI: 10.11646/zootaxa.4000.4.
De Stadt Emmerik
No full textdoor den vermaarden geschiedenisschrijver Everhardus Wassenberg in het jaar 1667 ... in 't latijn beschreeven. Door ... Vicarius Joannes van Wijck ... 1772 verkort, en in 't nederduitsch vertaald. Thans door ... Vicarius Franc. Xaverius Merbeck van veele taalfouten gezuivert ... verrijktVorlageform des Erscheinungsvermerks: uit drukkerij van J. L. RomenAusz
Maltose-binding protein from the hyperthermophilic bacterium Thermotoga maritima: Stability and binding properties
No full textRecombinant maltose-binding protein from Thermotoga maritima (TmMBP) was expressed in Escherichia coli and purified to homogeneity, applying heat incubation of the crude extract at 75 degrees C. As taken from the spectral, physicochemical and binding properties, the recombinant protein is indistinguishable from the natural protein isolated from the periplasm of Thermotoga maritima. At neutral pH, TmMBP exhibits extremely high intrinsic stability with a thermal transition >105 degrees C. Guanidinium chloride-induced equilibrium unfolding transitions at varying temperatures result in a stability maximum at approximate to 40 degrees C. At room temperature, the thermodynamic analysis of the highly cooperative unfolding equilibrium transition yields Delta G(N) --> (U) 100(+/-5) kJ mol(-1) for the free energy of stabilization. Compared to mesophilic MBP from E. coli as a reference, this value is increased by about 60 kJ mol(-1). At temperatures around the optimal growth temperature of T. maritima (t(opt) approximate to 80 degrees C), the yield of refolding does not exceed 80%; the residual 20% are misfolded, as indicated by ii decrease in stability as well as loss of the maltose-binding capacity. TmMBP is able to bind maltose, maltotriose and trehalose with dissociation constants in the nanomolar to micromolar range, combining the substrate specificities of the homologs from the mesophilic bacterium E.coli and the hyperthermophilic archaeon Thermococcus litoralis. Fluorescence quench experiments allowed the dissociation constants of Ligand binding to be quantified. Binding of maltose was found to be endothermic and entropy-driven, with Delta H-b = + 47 kJ mol(-1) and Delta S-b = + 257 J mol(-1) K-1. Extrapolation of the linear vant 'Hoff plot to t(opt) resulted in K-d approximate to 0.3 mu M. This result is in agreement with data reported for: the MBPs from E, coli and T, litoralis at their respective optimum growth temperatures, corroborating the general observation that proteins under their specific physiological conditions are in corresponding states. (C) 2000 Academic Press
FIGURE 1 in The rare mantis shrimp Areosquilla indica (Hansen, 1976) (Crustacea, Stomatopoda) from the Great Barrier Reef: first Australian records of the genus and species
No full textFIGURE 1. Areosquilla indica (Hansen, 1926), male, TL 38 mm (QM W27950). A, anterior cephalothorax; B, right eye; C, right dorsal process of antennular process, lateral view; D, left raptorial claw; E, right thoracic somites 5–8, dorsal view; F, right thoracic somite 5, lateral view; G, thoracic somite 8 sternal keel, right lateral view; H, posterior abdominal somites, telson and right uropod; I, right uropod, ventral view; J, right pleopod 1 endopod, anterior view. Scale A–F, H, I = 2.0 mm; G, J = 1.0 mm.Published as part of Ahyong, Shane T. & Wassenberg, Theodore J., 2015, The rare mantis shrimp Areosquilla indica (Hansen, 1976) (Crustacea, Stomatopoda) from the Great Barrier Reef: first Australian records of the genus and species, pp. 492-496 in Zootaxa 4000 (4) on page 493, DOI: 10.11646/zootaxa.4000.4.8, http://zenodo.org/record/23424
Letter, [Author unclear] to Paulina T. Merritt
No full textHandwritten letter to Paulina Merritt from an unknown author, October 1, 1876.
Climate change and Environmental awareness: a European working group to study the environmental impact and promote sustainability in Child Neurology
No full textClimate change is happening right now, and this is everyone’s problem. Beside the well-known negative impact on the environment, climate change directly and indirectly causes several health issues, that are more pronounced in low-income countries, in people with low socio-economic status, and in people with chronic and disabling conditions. As scientists, we feel the urge to spread awareness among the general population, and to persuade politicians and stakeholders on the necessity (and opportunities) to tackle climate issues for the sake of our planet. As clinicians, we need to promote our healthcare systems resilience and prevent climate-change related morbidity and mortality. As informed citizens, we must discourage inaction and promote engagement on environmental issues, at all levels: personal, social, financial, educational, political, medical and economical. To reach this target, the Young members of the European Pediatric Neurology Society (EPNS), strongly supported by the general EPNS board, have designed and coordinated the new “Climate change and environmental awareness Working Group”. Our aim is to educate on climate change-related health issues and promote positive actions for reduction of carbon emissions, damage mitigation, and adaptation plans within the field of Pediatric Neurology. Actions include networking with other scientific Societies and Groups for Climate defense (e.g., ILAE Climate change commission), performing literature revision and promoting multicenter studies on climate change-related health issues, estimating the Society carbon footprint (i.e. website, newsletters and in-person events) and foreseeing emissions compensation (e.g. with carbon credits), fostering sustainable alternatives for the biannual Society Congress like reducing paper use, proposing plant-based menus and discouraging international flights (e.g. hybrid in person and web-based congress, and easily-accessible-by-train locations). Climate change is everyone’s problem, and solutions need the effort of many. This is how we take action
Handwritten biographical information on Paulina T. McClung Merritt
No full textA handwritten biography of Paulina T. McClung Merritt by an unknown author, 1892.
Heterogeneous and tissue-specific regulation of effector T cell responses by IFN-gamma during Plasmodium berghei ANKA infection.
No full textIFN-γ and T cells are both required for the development of experimental cerebral malaria during Plasmodium berghei ANKA infection. Surprisingly, however, the role of IFN-γ in shaping the effector CD4(+) and CD8(+) T cell response during this infection has not been examined in detail. To address this, we have compared the effector T cell responses in wild-type and IFN-γ(-/-) mice during P. berghei ANKA infection. The expansion of splenic CD4(+) and CD8(+) T cells during P. berghei ANKA infection was unaffected by the absence of IFN-γ, but the contraction phase of the T cell response was significantly attenuated. Splenic T cell activation and effector function were essentially normal in IFN-γ(-/-) mice; however, the migration to, and accumulation of, effector CD4(+) and CD8(+) T cells in the lung, liver, and brain was altered in IFN-γ(-/-) mice. Interestingly, activation and accumulation of T cells in various nonlymphoid organs was differently affected by lack of IFN-γ, suggesting that IFN-γ influences T cell effector function to varying levels in different anatomical locations. Importantly, control of splenic T cell numbers during P. berghei ANKA infection depended on active IFN-γ-dependent environmental signals--leading to T cell apoptosis--rather than upon intrinsic alterations in T cell programming. To our knowledge, this is the first study to fully investigate the role of IFN-γ in modulating T cell function during P. berghei ANKA infection and reveals that IFN-γ is required for efficient contraction of the pool of activated T cells
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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