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Principle Ward E. Tibbet of Saqinaw High School meets with forum members to sponsor Luis Castellanos trip to Europe (photograph)
No full textPrinciple Ward E. Tibbet of Saqinaw High School meets with forum members to sponsor Luis Castellanos trip to Europe
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Antibody engineering: The use of Escherichia coli as an expression host
No full textThe hypervariable loops of an antibody molecule are supported on the relatively conserved β-sheeted frameworks of the heavy- and light-chain variable domains (designated VH and VL domains, respectively). Residues within and flanking these loops interact with antigen and confer the specificity and affinity of antigen binding on the immunoglobulin molecule. Thus, the isolation and expression of VH and VL domain genes are of particular interest both for analysis of the determinants of antibody specificity and for generation of fragments with binding affinities for use in therapy and diagnosis. The PCR can now be used to isolate diverse repertoires of antibody VH and VL domain genes from antibody-producing cells from different species, including humans and mice. The genes can be expressed as either secreted or surface-bound Fv or Fab fragments, using Escherichia coli expression systems, and the desired antigen-binding specificity screened for or, preferably, selected. The use of E. coli as an expression host allows the required antigen-binding specificity to be isolated in clonal form in a matter of days. The VH and VL domain genes can also be hypermutated and higher-affinity variants isolated by screening or selection. Thus, the use of this technology should allow the isolation of novel binding specificities or specificities that are difficult to generate by hybridoma technology. It will also facilitate the isolation of human-derived Fv/Fab fragments that may be less immunogenic in therapy. This approach therefore has almost unlimited potential in the generation of therapeutics with binding specificities to order. The fragments can be used either alone or linked to effector functions in the form of antibody-constant domains or toxins. The new technology could prove to be a method of choice for the rapid and convenient production of designer antibodies.</p
VH shuffling can be used to convert an Fv fragment of anti-hen egg lysozyme specificity to one that recognizes a T cell receptor Vα
No full textThis study describes the isolation and characterization of Fv fragments that recognize a T cell receptor Vα (Vα 1934.4). A VH gene repertoire from an immunized mouse was recombined with the anti-hen egg lysozyme (HEL) VκD1.3 gene as single chain (sc)Fvs, and an Fv with reasonable affinity for binding to Vα1934.4 isolated. The Fv (VH14/VκD1.3) does not bind to HEL, indicating that the heavy chain shuffling has converted an anti-HEL specificity to one that recognizes the unrelated Vα1934.4. The association constant for the Fv-Vα1934.4 interaction has been determined using surface plasmon resonance (SPR) and is 1.2 × 107 M-1. Recombinant antibodies of reasonable affinity can therefore be generated by combining a VH library with a 'fixed' Vκ. To improve the affinity further, light chain shuffling has been used to generate an Fv (VH14/Vκ9) that has a 30-fold higher affinity for binding to Vα1934.4 than the parent (VH14/VκD1.3) Fv, and SPR measurements demonstrate that the affinity improvement is due to an increase in on-rate. Unexpectedly, Vκ9 differs from VκD1.3 by only two amino acids at positions 30 and 91 and, consistent with the change in binding affinity, both of these residues are located in CDRs.</p
Expression and secretion of T‐Cell receptor Vα and Vβ domains using Escherichia coli as a host
No full textAn expression system for the production of recombinant T‐cell receptor (TCR) variable domains would, inter alia, allow structural studies lo be carried out and provide protein for the generation of anti‐clonotypic antibodies, in this report the Vα and Vβ domain genes have been isolated from a T‐cell hybridoma which is associated with the pathogenesis of experimental allergic encephalomyelitis (EAE) In the H‐2 mouse. These have been expressed as secreted domains in Escherichia coli, using secretion vectors previously used for the production of immunoglobulin fragments. Both Vα and Vβ domains are secreted in milligram quantities into the culture supernatant, although the levels of the Vα domain are about 10‐20 Ibid higher than those of the Vβ domain. This expression system offers a rapid route for the production of recombinant TCRs in soluble form.</p
Dreaming of a castle in the air [music] /
No full textFor voice and piano.; Caption title.; "Arrangement for 'Banjulele' banjo and ukulele by Kel Keech."; Covers vary.; NLA's "Snell N" copy: "Featured by the famous English, Humphrey Bishop Musical Comedy Co. Sung by Thelma Trott"--Cover. Portraits of Thelma Trott and Humphrey Bishop on cover. ANL; NLA's "N" copy: "Sung by Rene Dixon. Sir Benjamin Fuller and Mr. John Fuller present London's beautiful fairy pantomime, 'Jack and the beanstalk' at the St. James Theatre"--Cover. Portraits of Sir Ben. Fuller, Mr. John Fuller and Rene Dixon on cover. Col. ill. of Jack climbing the beanstalk on cover. ANL; Also available online http://nla.gov.au/nla.mus-an6301606; 1925 by Harms Inc
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Secretion of T cell receptor fragments from recombinant Escherichia coli cells
No full textThis study describes the secretion and purification of T cell receptor (TCR) Vα, Vβ domains and single chain Vα-Vβ fragments (scTCRs) from recombinant Escherichia coli cells. The TCR Vα and Vβ genes are derived from a T cell hybridoma that is associated with disease pathogenesis in murine experimental allergic encephalomyelitis (EAE). Circular dichroism (c.d.) analyses of the single domains and the scTCR indicate that they are folded into β-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted TCR fragments can be purified in milligram quantities, and could therefore be used in high-resolution structural studies, in immunization to generate anti-clonotypic antibodies or in vaccination.</p
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