1,722,784 research outputs found
Nü si shu ji zhu : 4 juan. , Cao da jia nü jie / Wang Xiang jian zhu ; Zheng Han jiao zi曹大家女誡 / 王相箋註 ; 鄭漢校梓
No full textComprend : Song ruo zhao nü lun yu / Wang Xiang jian zhu ; Zheng Han jiao zi - Wang jie fu nü fan jie lu / Wang Xiang ding zhu - Ren xiao wen huang hou nei xun / Wang Xiang jian zhu - 宋若昭女論語 / 王相箋註 ; 鄭漢校梓 - 王節婦女範㨗錄 / 王相訂註 - 仁孝文皇后內訓 / 王相箋註Avec mode text
Single-cell impedance spectroscopy of bacteria
No full textBacteria are the causative agents of many diseases, and understanding biophysical changes in bacteria following physical or chemical treatment is of considerable interest. In this thesis, different bacteria were measured using single-cell impedance cytometry in order to understand changes in electrical properties following exposure to different perturbations. Traditionally, alternating current (AC) electrokinetic methods are used to measure the electrical properties of cells. However, the throughput of these techniques is low. To overcome this limitation single-cell impedance cytometry measures the impedance of individual cells at hundreds per second.In this study, the electrical properties of single cells were measured across a broad range of frequencies at the rate of tens of thousands of cells in a few minutes. The complex impedance spectrum was modelled using Maxwell’s mixture equation together with the multi-shell model to extract dielectric parameters for each bacterium.Two model organisms were used for experiments: Escherichia coli and Staphylococcus aureus. Impedance cytometry was used to characterise the effect of heat treatment (including pasteurisation and autoclaving) on bacteria and results showed that the dielectric properties of the cells were in agreement with AC electrokinetic methods. Bacteria were also exposed to three classes of antibiotics, namely β-lactam, polymyxin and aminoglycoside. Dielectric characterisation demonstrated that bacteria respond differently following exposure to different antibiotics. Their phenotypic response changes their dielectric parameters in a way that is consistent with the mode of action of antibiotics.Single-cell impedance cytometry was also used to follow bacterial-phage interaction. Experiments showed that the phenotypic response of bacteria can be followed during the phage infection cycle, and that the technique can rapidly identify bacterial susceptibility to phages. The data also helps explain the complex bacterial defence mechanisms against phages from a dielectric perspective.In summary, this thesis describes an electrical method for probing bacteria, linking measured biophysical changes to biological responses, furthering our understanding of the action of antibiotics and phage therapies
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
New dictyopteran fossils from the Pennsylvanian of West Beijing, China
No full textHuang, Di-ying, Wei, Guo, Wei, Tao, Wu, Yi, Wang, Xiang-dong, Li, Xin-ran (2022): New dictyopteran fossils from the Pennsylvanian of West Beijing, China. Palaeoentomology 5 (2): 161-166, DOI: 10.11646/palaeoentomology.5.2.9, URL: http://zoobank.org/69aff1dc-743a-4270-a706-523333c4089
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
FIGURE 4. A in New dictyopteran fossils from the Pennsylvanian of West Beijing, China
No full textFIGURE 4. A new dictyopteran fossil (NIGP180000) preserved with overlapped fore and hind wings. A, Part. B, Counterpart. Scale bars = 5 mm.Published as part of Huang, Di-ying, Wei, Guo, Wei, Tao, Wu, Yi, Wang, Xiang-dong & Li, Xin-ran, 2022, New dictyopteran fossils from the Pennsylvanian of West Beijing, China, pp. 161-166 in Palaeoentomology 5 (2) on page 165, DOI: 10.11646/palaeoentomology.5.2.9, http://zenodo.org/record/653049
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