26,804 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Full text linkThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Wang Meng and contemporary Chinese literature: the vicissitudes of a committed writer
Full text linkThis thesis examines the way Wang Meng has developed as a writer from the 1950s to the 1990s in the context of New China's political and literary background. It looks at the compromises he was forced to make between his political beliefs in the Communist Party and his chosen role as a professional writer. After his disastrous early foray into what was deemed to be unacceptable political criticism with The Young Newcomer in the Organisation Department in the 1950s, when the opportunity came to start publishing again in the late 1970s he was boldly innovative in style, helping to transform New Period literature, but conservative in content, sticking to politically acceptable topics. It was only with Hard Porridge in 1989 that he ventured again, and very successfully, into political comment. There is no outstanding leading writer in contemporary China, but Wang Meng is a leading contender for the title
Isolation and identification of Salmonella enterica serotype enteritidis from a layer breeder farm.
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Phylogenetic analysis of subgroup J avian leucosis virus from broiler and native chickens in Taiwan during 2000-2002
No full textFrom Gui Bo Shang Tong to Ji Wang Qiu Shi\ue2A Study of Xing Zhi
No full textIntegrated with the study of Chinese Classics, the Ch\ue2en-wei thoughts posed as the mainstream of East Han\ue2s philosophy studies but the thoughts also aroused different opinions. As early as in the beginning of West Han Dynasty, Liu Shin tried to oppose the studies of Modern Scripts with the studies of Ancient Scripts. Yang Xiong, a great thinker in late West Han Dynasty, tried to suppress the popularity of superstition with the concept of Dao Fa Zi Ran (meaning that Daoism lies in the Nature). In the beginning of East Han Dynasty, Heng Tan is the first thinker who openly criticized the official philosophy. He wrote \ue2Xing Lun\ue2 to declare his opposition against the dominant Ch\ue2en-wei thoughts and also offered suggestions about contemporary politics in the hope of attracting attention from the Emperor and serving the country. However, his suggestions irritated the Emperor instead and he was never promoted to an important position. After Heng Tan, Wang Chong, a thinker not in any official post, carried forward Heng\ue2s thoughts and, based on the principle of Ji Xu Wang (meaning to criticize the untrue), lashed even more strongly against the Ch\ue2en-wei thoughts.
This thesis is an attempt to analyze Heng\ue2s and Wang\ue2s thoughts based on their books, \ue2Xing Lun\ue2 and \ue2Lun Heng.\ue2 It is found that both of them, enlightened by rationalism and spurred by the sense of mission as orthodox scholars, tried to fight against the contemporary Boshi system on the basis of Bo Tong (meaning comprehensive studies), to criticize the ridiculousness of the Ch\ue2en-wei thoughts by manifesting the principle of Ji Xu Wang and finally to achieve the ideal of Xing Zhi (meaning to criticize the untrue and to serve the country with one\ue2s thoughts). Both Heng and Wang cast a deep influence on the Ming-Fa thoughts at the end of East Han Dynasty and gave birth to the following Naturalism. Their importance to the development of Chinese philosophy can be clearly seen hereby
Fractionation of the Methane Isotopologues ¹³CH₄, ¹²CH₃D, and ¹³CH₃D During Aerobic Oxidation of Methane by Methylococcus Capsulatus (Bath)
Full text linkAerobic oxidation of methane plays a major role in reducing the amount of methane emitted to the atmosphere from freshwater and marine settings. We cultured an aerobic methanotroph, Methylococcus capsulatus (Bath) at 30 and 37 °C, and determined the relative abundance of ¹²CH₄, ¹³CH₄, ¹²CH₃D, and ¹³CH₃D (a doubly-substituted, or “clumped” isotopologue of methane) to characterize the clumped isotopologue effect associated with aerobic methane oxidation. In batch culture, the residual methane became enriched in ¹³C and D relative to starting methane, with D/H fractionation a factor of 9.14 (Dε/13ε) larger than that of ¹³C/¹²C. As oxidation progressed, the Δ¹³CH₃D value (a measure of the excess in abundance of ¹³CH₃D relative to a random distribution of isotopes among isotopologues) of residual methane decreased. The isotopologue fractionation factor for ¹³CH₃D/¹²CH₄ was found to closely approximate the product of the measured fractionation factors for ¹³CH₄/¹²CH₄ and ¹²CH₃D/¹²CH₄ (i.e., ¹³C/¹²C and D/H). The results give insight into enzymatic reversibility in the aerobic methane oxidation pathway. Based on the experimental data, a mathematical model was developed to predict isotopologue signatures expected for methane in the environment that has been partially-oxidized by aerobic methanotrophy. Measurement of methane clumped isotopologue abundances can be used to distinguish between aerobic methane oxidation and alternative methane-cycling processes. Keywords: Methane; Clumped isotopologues; Aerobic methane oxidation; Methanotrophic cultures; Isotopic fractionationNational Science Foundation (U.S.) (Grant EAR-1250394
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