45 research outputs found
In vitro visualization of human endodontic structures using different endoscope systems
No full textDifferent endoscope optics for the visualization of interradicular structures were evaluated as a diagnostic tool. A sample of 20 extracted human lower molar teeth was used. Only teeth with fully formed apices were included. All samples were evaluated with three different endoscopic procedures: pulp endoscopy (PE), canal entrance endoscopy (CEE) and root canal endoscopy (RCE). All pulp chambers could be observed using PE (100%), however, only 41 of 60 (68.3%) canals were observed. With CEE, all entrances could be observed, and the middle third of the canals could be visualized in 85% of the canals. The semiflexible endoscope for RCE allowed successful observation of 91.6% of the middle third of the canals. The application of the endoscope may be useful in the identification of root canals even under difficult visual work field conditions. The combined use of a set of various optics might enable the operator to enhance the quality of non-surgical endodontic procedures
High Mobility Group Box 1 Protein in Osteoarthritic Knee Tissue and Chondrogenic Progenitor Cells: An Ex Vivo and In Vitro Study
No full textObjective In osteoarthritis (OA), a loss of healthy cartilage extracellular matrix (ECM) results in cartilage degeneration. Attracting chondrogenic progenitor cells (CPCs) to injury sites and stimulating them toward chondrogenic expression profiles is a regenerative approach in OA therapy. High mobility group box 1 protein (HMGB1) is associated with chemoattractant and proinflammatory effects in various pathological processes. Here, we investigate the migratory effects of HMGB1 in knee OA and CPCs for the first time. Design Immunohistochemistry, immunoblotting, and immunocytochemistry were performed to identify HMGB1 and its receptors, receptor for advanced glycation end products (RAGE) and toll-like receptor 4 (TLR4) in OA knee tissue, chondrocytes, and CPCs. In situ hybridization for HMGB1 mRNA was performed in CPCs ex vivo. The chemoattractant effects of HMGB1 on CPCs were analyzed in cell migration assays. Results HMGB1 expression in OA tissue and OA chondrocytes was higher than in healthy specimens and cells. HMGB1, RAGE, and TLR4 were expressed in CPCs and chondrocytes. In situ hybridization revealed HMGB1 mRNA in CPCs after migration into OA knee tissue, and immunohistochemistry confirmed HMGB1 expression at the protein level. Stimulation via HMGB1 significantly increased the migration of CPCs. Conclusions Our results show the chemoattractant role of HMGB1 in knee OA. HMGB1 is released by chondrocytes and has migratory effects on CPCs. These effects might be mediated via RAGE and TLR4. The in vitro and ex vivo results of this study need to be confirmed in vivo.Deutsche Forschungsgemeinschaft https://doi.org/10.13039/50110000165
One-Piece Titanium Implants: Retrospective Case Series
No full textPurpose. One-piece titanium implants are not routinely used for reconstruction after tooth loss. Several limitations seemed to be apparent although the concept provides a straightforward approach for different clinical situations. A clinical documentation of five prosthetic restorations with one-piece titanium implants serving as a relevant treatment option in dental surgery is pursued. We demonstrate the feasibility and benefits of one-piece titanium implants for fixed dental prosthesis. Detailed descriptions of the technical features and the surgical approach by means of clinical cases are given. The prosthetic workflow when working with one-piece titanium implants is depicted in detail as well as examples for implant-supported tooth replacement in the posterior region and the esthetic zone. Conditions of applications regarding different timing of implant placement using the system and its limitations are discussed. Results. Clinical cases with a follow-up period of up to 10 years are presented to prove the long-term success of one-piece titanium implants in terms of bone and soft-tissue stability respecting the biological criteria for periodontal health. Conclusions. One-piece titanium implants represent a reliable treatment method for single-tooth replacements. Clinical success with long-time bone stability around the implantation site can be achieved. Taken into account the requirements for periodontal tissue stability, uneventful healing without extensive tissue loss is demonstrated by means of clinical cases presenting patients with periodontitis
Expression analysis of chondrogenic progenitor cells in ex-vivo migration experiment
No full textThe chondrogenic progenitor cells are under control of the expression factors Sox9 and Runx2 favoritising either chondrogenic or osteogenic behavior. In this study we examine the impact on chondrogenic progenitor cells with a Runx2 knockdown. Using a migration experiment we could analyse the expression of collagen typ 1 and 2 of that cells.2015-05-2
Practical Aspects of Processing and Interpretation of the Results of Microbiological Analysis of the Quality of Non-Sterile Medicines
No full textThe article presents materials on processing and interpretation of the results of microbiological analysis of the quality of medicines in accordance with the State Pharmacopoeia of the Russian Federation and the Pharmacopoeia of the Eurasian Economic Union. It exemplifies the use of the rules for processing test results and counting aerobic microorganisms and microscopic fungi on agar culture media in Petri dishes and on membrane filters. The author discusses the possibility of and prospects for applying various sample inoculation methods, as well as using calculation factors when interpreting their results. The text covers approaches to repeating microbiological testing of medicines to verify the reliability of a non-compliant first result
Aspectus et Affectus: Essays and Editions in Grosseteste and Medieval Intellectual Life in Honor of Richard C. Dales
No full textR. James Long is a contributing author, A Thirteenth Century Teaching Aid: An Edition of the Bodleian Abbreviatio of the Pseudo Aristotelian De plantis, , pp. 87-103.
Book description: The 65th year of a scholar who has devoted 40 years to editing and elucidating Robert Grosseteste provides us with a collection of essays. Not surprisingly, they emanate from colleagues and former students of Richard Dales and reflect his interest, among other concerns, in Grosseteste\u27s aspectus et affectus - range of vision and disposition of mind - those twin peaks with which the 13th century thinker helped to get Christian thought through Aristotle without mutual destruction. Joining Sir Richard Southern are 8 scholars whose contributions fill two parts of this book, Robert Grosseteste and Medieval Intellectual Life . In addition to essays on Grosseteste are articles on Hugh of Amiens, Alfred of Sareschel\u27s commentary on Avicenna, the Oxford Condemnations of 1277, a 13th-century teaching aid, and canonistic determinations on the stages of childhood
Managing Cache Memory Resources in Adaptive Many-Core Systems
No full textPart 4: Memory System DesignInternational audienceIn the last decades, the increasing amount of resources in embedded systems has been leading them to the point where an efficient management of these resources is mandatory, especially for the memory subsystem. Current MPSoCs have more than one application running concurrently. Hence, it is important to identify the memory needs of these applications and provide them accordingly. In this work we propose the use of a cluster-based, resource-aware approach to provide this efficient environment. The solution proposed here improves the overall performance of these systems by aggregating memory resources in clusters and redistributing these resources among applications based on a fairness criterion. For this memory clustering proposal, we use the information of external memory access-es as an estimate of the amount of memory required by each application. T Experimental results show that, depending on how the redistribution of memory resources among application occurs, the overall system can improve performance up to 18% and the energy savings can reach up to 20%
Практические аспекты учета и интерпретации результатов микробиологического анализа качества нестерильных лекарственных средств
No full textThe article presents materials on processing and interpretation of the results of microbiological analysis of the quality of medicines in accordance with the State Pharmacopoeia of the Russian Federation and the Pharmacopoeia of the Eurasian Economic Union. It exemplifies the use of the rules for processing test results and counting aerobic microorganisms and microscopic fungi on agar culture media in Petri dishes and on membrane filters. The author discusses the possibility of and prospects for applying various sample inoculation methods, as well as using calculation factors when interpreting their results. The text covers approaches to repeating microbiological testing of medicines to verify the reliability of a non-compliant first result.Представлены материалы, касающиеся учета и интерпретации результатов микробиологического анализа качества лекарственных средств в соответствии с требованиями Государственной фармакопеи Российской Федерации и Фармакопеи Евразийского экономического союза. На примерах показано использование правил учета результатов и расчета количества аэробных микроорганизмов и микроскопических грибов на агаризованной питательной среде в чашках Петри и на мембранных фильтрах. Обсуждаются возможности и перспективы использования различных методов посева образцов и применения расчетных коэффициентов при интерпретации результатов. Рассмотрены подходы к повторному микробиологическому испытанию лекарственных средств в случае первичного выявления несоответствия, которое требует подтверждения для получения достоверного результата
Impact of Immunosuppressive Drugs on Fibroblasts: An In Vitro Study
No full textBackground: The aim of this study was to compare the direct impact of different agents for immunosuppressive therapy on mouse fibroblasts as a possible cause of drug-induced gingival overgrowth (DIGO). Methods: 3T3 mouse fibroblasts were cultivated in cell-specific media (2 × 104 cells/mL) and treated for 6, 24, 48 and 72 h with one of three immunosuppressive drugs (IsDs): cyclosporin a (CsA), tacrolimus (TaC) and sirolimus (SiR). Different concentrations (10–750 ng/mL) were used to mimic serum levels under active immunosuppressive therapy conditions. Cell population characteristics (cell number, viability and morphology) were assessed using computer-assisted cell analysis. Expression of pro-collagen type I carboxy-terminal propeptide (PICP) was identified using an ELISA assay. Results: The influence of IsDs on the biological status of 3T3 fibroblasts was time- and dose-dependent. Comparing CsA and TaC, the total cell amount was enhanced using concentrations in the range of 10–150 ng/mL (p > 0.05). In contrast, treatment with SiR resulted in a decrease in the average cell number (p < 0.01). PICP and cell diameter of fibroblasts were not susceptible to IsD treatment (p > 0.05). Conclusions: Our results revealed time-dependent effects of IsDs, with distinct influences on cell number. The cell morphology and the PICP balance of the investigated fibroblast cell line remained unaffected. Hence, the potential role of IsDs is not a unilateral mechanism of action but rather a multifactorial process.the German Research Foundation (DFG)Leipzig Universit
Influences of cold atmospheric plasma on apoptosis related molecules in osteoblast-like cells in vitro
No full textAbstract Background Cold atmospheric plasma (CAP) has recently been identified as a novel therapeutic strategy for supporting processes of wound healing. Since CAP is additionally known to kill malignant cells, our study intends to determine the influence of CAP on crucial molecules involved in the molecular mechanism of apoptosis in osteoblast-like cells. Methods Human osteoblast-like cells were CAP-treated for 30 and 60 s. CAP effects on critical factors related to apoptosis were studied at transcriptional and protein level using real time-PCR, immunofluorescence staining and western blot. Phalloidin / DAPI staining was used for analyzing the cell morphology. In addition, apoptotic outcomes of CAP were displayed using flow cytometry analysis. For studying intracellular signaling pathways, MAP kinase MEK 1/2 and PI3K were blocked. Finally, the effects of CAP on caspase-3 activity were examined using a caspase-3 assay. Results CAP treatment resulted in a significant downregulation of p53 and apoptotic protease activating factor (APAF)-1, caspase (CASP)9, CASP3, BCL2 Antagonist/Killer (BAK)1, and B-Cell Lymphoma (BCL)2 mRNA expression at 1 d. An inhibitory effect of CAP on apoptotic genes was also shown under inflammatory and apoptotic conditions. Nuclear translocation of p53 was determined in CAP treated cells at the early and late stage, after 15 min, 30 min, and 1 h. p53 and APAF-1 protein levels were reduced at 1 d, visualized by immunofluorescence and western blot, respectively. Moreover, a morphological cytoskeleton modification was observed after CAP treatment at 1 d. Further, both CAP-treated and untreated (control) cells remained equally vital as detected by flow cytometry analysis. Interestingly, CAP-associated downregulation of CASP9 and CASP3 mRNA gene expression was also visible after blocking MAP kinase and PI3K. Finally, CAP led to a decrease in CASP3 activity in osteoblast-like cells under normal and apoptotic conditions. Conclusions Our in vitro-study demonstrated, that CAP decreases apoptosis related molecules in osteoblast-like cells, underlining a beneficial effect on hard-tissue cells
