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Volume regulation in HeLa cells: role of ion transport.
Get PDFby Wong Chi Shing Micky.Thesis (M.Phil.)--Chinese University of Hong Kong, 1996.Includes bibliographical references (leaves 140-149).Chapter Chapter 1. --- Introduction --- p.1Chapter 1.1 --- The Uterine Cervix --- p.1Chapter 1.2 --- Cervical Secretion and its Function --- p.3Chapter 1.3 --- Ion Transport System in Cell Volume Regulation --- p.6Chapter 1.3.1 --- "Regulatory Volume Decrease, RVD" --- p.8Chapter 1.3.2 --- "Regulatory Volume Increase, RVI" --- p.8Chapter 1.4 --- Signaling Pathways underlying RVD and RVI - The Role of Intracellular Free Calcium --- p.11Chapter 1.5 --- Swelling-induced Cl- Current --- p.12Chapter 1.6 --- Ca2+ activated K+ Channel --- p.14Chapter 1.7 --- Objectives of the Study --- p.15Chapter Chapter 2. --- Materials and Methods --- p.17Chapter 2.1 --- Materials --- p.17Chapter 2.1.1 --- Culture Media --- p.17Chapter 2.1.2 --- Chemicals --- p.17Chapter 2.2 --- Preparation of Solutions --- p.18Chapter 2.3 --- Cell Culture --- p.21Chapter 2.4 --- Patch-clamp Technique --- p.22Chapter 2.4.1 --- Preparation of Electrode --- p.27Chapter 2.4.1.1 --- Pulling and Polishing of Electrode --- p.27Chapter 2.4.1.2 --- Filling of the Electrode --- p.27Chapter 2.4.1.3 --- Coating of Electrode --- p.28Chapter 2.4.2 --- Patch-clamp Study --- p.29Chapter 2.4.2.1 --- Formation of Whole-cell Configuration --- p.29Chapter 2.4.2.2 --- Data Acquisition and Analysis --- p.31Chapter 2.5 --- Study of Cellular Volume Regulation by Confocal Laser Scanning Microscopy (CLSM) --- p.35Chapter 2.6 --- Determination of Intracellular Ca2+ by Confocal Laser Scanning Microscopy (CLSM) --- p.38Chapter Chapter 3. --- Results --- p.39Chapter 3.1 --- "Regulatory Volume Decrease, RVD" --- p.39Chapter 3.2 --- Responses of [Ca2+］i to swelling --- p.47Chapter 3.3 --- KC1 Efflux in RVD in HeLa Cells --- p.57Chapter 3.4 --- Swelling-induced Cl- Current --- p.67Chapter 3.4.1 --- Swelling-induced Anion and Cation Current --- p.67Chapter 3.4.2 --- Ca2+-independence of Swelling-induced Cl- Current --- p.71Chapter 3.4.3 --- Effect of Cl- Channel Blockers on Swelling-induced Cl- Current --- p.75Chapter 3.4.4 --- Anion Selectivity of Swelling-induced Cl- Current --- p.85Chapter 3.4.5 --- Cl- Dependence of Swelling-induced Cation Conductance --- p.85Chapter 3.4.6 --- K+ Independence of Swelling-induced Anion Conductance --- p.95Chapter 3.5 --- Ca2+ Activated K+ Current --- p.101Chapter 3.5.1 --- Ionomycin Induced Cell Shrinkage under Isotonic Condition --- p.101Chapter 3.5.2 --- Ionomycin Stimulated a Whole-cell K+ Conductance --- p.101Chapter 3.5.3 --- The Effect of Ionomycin on Intracellular Ca2+ Level --- p.111Chapter 3.5.4 --- Ca2+ Dependence of Ionomycin Stimulated K+ Current --- p.111Chapter Chapter 4. --- Discussion --- p.124Regulatory Volume Decrease in HeLa Cells --- p.124Role of Calcium in Regulatory Volume Decrease (RVD) --- p.126Swelling-induced Cation and Anion Conductance --- p.128Ca2+ Activated K+ Current in HeLa Cells --- p.134Chapter Chapter 5. --- Reference --- p.14
Underway physical oceanography and carbon dioxide measurements during LILOOET cruise Lil498N
No full textCruise QC flag: D (see further details
Socialist Republic of Vietnam: Wong Chi Shing
No full textA document from Amnesty International's Urgent Action Networ
Carbon, Nitrogen and Silica Particle Flux of OSP_trap
No full textCarbon, Nitrogen and Silica Particle Flux of OSP_tra
Underway physical oceanography and carbon dioxide measurements mooring Station P, STNP-7505
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Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Primary production and chlorophyll of station P26 (Papa)
No full textPrimary production and chlorophyll of station P26 (Papa
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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