5 research outputs found
Efek Serat Bengkuang (Pachyrhizus erosus L.) Dalam Pakan Berlemak Tinggi Terhadap Indikator Inflamasi pada Limpa Mencit Putih
No full textSerat bengkuang (Pachyrhizus erosus L.) telah terbukti dapat mencegah terjadinya obesitas dan berperan sebagai imunodulator. Akan tetapi,efek serat bengkuang (P. erosus L.) dalam pakan berlemak tinggi (PLT) terhadap indikator inflamasi pada limpa belum diketahui dengan jelas. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian serat umbi bengkuang (P. erosus L.) dalam PLT terhadap perubahan berat badan, berat dan indeks organ limpa serta indikator-indikator inflamasi yang meliputi perubahan proporsi area relatif pulpa putih limpa, peningkatan jumlah multinuclear giant cell (MGC) dan jumlah makrofag bervakuola pada jaringan limpa mencit. Penelitian ini telah dilakukan dari bulan Maret sampai Agustus 2021 secara eksperimen menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan enam kali ulangan terhadap hewan uji mencit putih jantan galur DDY. Mencit diberikan perlakuan pakan normal (PN), PLT, PLT ditambah masing-masing serat bengkuang (SB) 10% dan 25%. Hasil dari penelitian ini yaitu pemberian serat bengkuang (P. erosus L.) dalam PLT dapat mencegah peningkatan berat badan mencit secara signifikan akan tetapi tidak memberikan efek yang berbeda nyata terhadap perubahan berat dan indeks limpa dibandingkan dengan mencit yang diberi pakan normal, dapat mencegah peningkatan jumlah MGC dan makrofag bervakuola pada jaringan limpa mencit secara signifikan serta mencegah penurunan proporsi area relatif pulpa putih limpa. Kesimpulan dari penelitian ini bahwa serat bengkuang (P. erosus L.) dalam PLT memiliki efek yang menguntungkan dalam mencegah inflamasi dengan menurunkan jumlah MGC dan makrofag bervakuola secara signifikan pada jaringan limpa dengan dosis terbaik yaitu 25%
Preventive Effect of Jicama (Pachyrhizus erosus L.) Fiber in High-Fat Diet Against Oxidative Stress and Inflammation on The Intestinum of White Mice (Mus Musculus L.)
No full textJicama (Pachyrhizus erosus L.) fiber has been shown to prevent oxidative stress and inflammation in the development of obesity. Efficacy of jicama fiber on indicators of oxidative stress and inflammation in the intestine, intestinal histopathology, hematological profiles and its mechanism in inhibiting the inflammatory signalling pathway is not clearly known. This study aimed to analyze the effect of jicama fiber supplementation on oxidative stress indicator, histophatology and inflammation of intestine, hematological profiles and analyze the ability of bioactive compounds in jicama fiber as anti-inflammatory trough molecular docking simulation. This research was carried out from April to August 2022 experimentally using completely randomized design (CRD) with three treatments and nine replications on male white mice DDY strain. Mice were treated with normal diet (ND), high-fat diet (HFD), HFD combined with 25% of jicama fiber. The results of this study demonstrated that the suplementation of jicama fiber in HFD could prevent significantly an increase malondialdehyde (MDA) level and a decrease catalase activity, prevent the histophatology alteration and inflammation on the intestinal tissue of mice, prevent an increase total leukocytes count and a decrease in the mean cospular hemoglobin concentration (MCHC) as well as bioactive compounds in jicama fiber particularly cycloartenol and astaxanthin have a potential to inhibit activation of inflammatory signalling pathway based on molecular docking simulation. It is concluded that supplementation jicama fiber 25% in HFD, could exert a beneficial effect in preventing oxidative stress and inflammation in the intestine of white mice.
Keyword: Astaxanthin, Catalase Activity, Cycloartenol, MDA, Total Leukocytes
EFEK EKSTRAK BENGKUANG (Pachyrhizus erosus L.) DALAM PAKAN BERLEMAK TINGGI TERHADAP INDIKATOR INFLAMASI PADA LIMPA MENCIT PUTIH.
No full textviii, 74 hal
PREVENTIVE EFFECT OF JICAMA (Pachyrhirus erosus L.) FIBER IN HIGH-FAT DIET AGAINST OXIDATIVE STRESS AND INFLAMMATION ON THE INTESTINUM OF WHITE MICE (Mus musculus L.).
No full textxvi, 112 hal
In Silico Study of Anti-inflammatory Effect of Jicama (Pachyrhizus erosus L., Fabaceae) Tuber Fiber
No full textUncontrolled inflammation is a serious problem associated with various pathological conditions and must be treated appropriately. Jicama (Pachyrhizus erosus L., Fabaceae) fiber has been shown to exert an immunomodulatory effect and contain bioactive compounds that act as anti-inflammatory. However, the mechanism of jicama fiber in inhibiting the inflammatory signaling pathway is unknown. This study aimed to investigate the ability of bioactive compounds in jicama fiber as anti-inflammatory by molecular docking. The molecular docking was performed using PyRx 0.8 with the Vina algorithm and using BIOVIA Discovery Studio v21.1.0.20298 and PyMOL v1.7.4 to visualize the docking results and to analyze the conformation and interaction of ligands and receptor. This in silico test tethered eight bioactive compounds that have the potential as anti-inflammatory in Jicama fiber as ligands with toll-like receptor-4 (TLR4) as a receptor involved in the inflammatory response. The result demonstrated that Cycloartenol has the lowest binding affinity and the highest number of amino acid residues while interacting with TLR4 as compared to the other ligands namely Dexamethasone (a standard ligand), Stemphol, Astaxanthin, Farnesol, Benzoic Acid, 2.6-Dihydroxibenzoic Acid, Nerolidol, and 9-Octadecenoic Acid. Our recent study revealed that bioactive compounds in jicama fiber particularly Cycloartenol have the potential to inhibit inflammation through the inactivation of TLR4 which is the central signaling system in the inflammatory response
