434 research outputs found
MSI-testing in hereditary non-polyposis colorectal carcinoma (HNPCC)
No full textGenomic instability at simple repeated sequences, termed microsatellite instability (MSI). plays an important role in the analysis of sporadic and hereditary colon cancers. In hereditary non-polyposis colorectal cancer syndrome (HNPCC) more than 90% of cases show MSI, whereas only 10-15% of sporadic colorectal cancers do so. Thus. microsatellite analysis is commonly used as the first diagnostic screening test for HNPCC. In 1997, an international collaborative workshop sponsored by the National Cancer Institute (NCI) proposed a set of guidelines for MSI-testing to improve reliability and reproducibility of the analysis as well to allow comparisons between different studies and different laboratories. In this review we assess the value of current protocols for MSI-testing and discuss some diagnostic pitfalls. Our findings support continued use of the MSI marker panel recommended in 1997. Additionally, MSI-testing should be. improved by use of microdissection, which helps to identify additional patients with MSI due to enrichment of tumor cells and therefore increased sensitivity. In our view. immunohistochemical staining for mismatch repair protein expression is not a substitute for MSI-analysis but complements MSI screening and helps direct further testing. In summary, MSI-analysis is a highly sensitive and reliable screening method for HNPCC, that requires a well-equipped laboratory, as well as an experienced pathologist. Integration of family history and histo-pathological features is also critical.NCI NIH HHS [R01 CA067007
“Development of Risk Mitigation Guidance for Sensor Placement Inside Mechanically Ventilated Enclosures Phase 1”
No full textMSI-testing in hereditary non-polyposis colorectal carcinoma (HNPCC)
No full textGenomic instability at simple repeated sequences, termed microsatellite instability (MSI). plays an important role in the analysis of sporadic and hereditary colon cancers. In hereditary non-polyposis colorectal cancer syndrome (HNPCC) more than 90% of cases show MSI, whereas only 10-15% of sporadic colorectal cancers do so. Thus. microsatellite analysis is commonly used as the first diagnostic screening test for HNPCC. In 1997, an international collaborative workshop sponsored by the National Cancer Institute (NCI) proposed a set of guidelines for MSI-testing to improve reliability and reproducibility of the analysis as well to allow comparisons between different studies and different laboratories. In this review we assess the value of current protocols for MSI-testing and discuss some diagnostic pitfalls. Our findings support continued use of the MSI marker panel recommended in 1997. Additionally, MSI-testing should be. improved by use of microdissection, which helps to identify additional patients with MSI due to enrichment of tumor cells and therefore increased sensitivity. In our view. immunohistochemical staining for mismatch repair protein expression is not a substitute for MSI-analysis but complements MSI screening and helps direct further testing. In summary, MSI-analysis is a highly sensitive and reliable screening method for HNPCC, that requires a well-equipped laboratory, as well as an experienced pathologist. Integration of family history and histo-pathological features is also critical.NCI NIH HHS [R01 CA067007
Development of risk mitigation guidance for sensor placement inside mechanically ventilated enclosures – Phase 1
No full textGuidance on Sensor Placement was identified as the top research priority for hydrogen sensors at the 2018 HySafe Research Priority Workshop on hydrogen safety in the category Mitigation, Sensors, Hazard Prevention, and Risk Reduction. This paper discusses the initial steps (Phase 1) to develop such guidance for mechanically ventilated enclosures. This work was initiated as an international collaborative effort to respond to emerging market needs related to the design and deployment equipment for hydrogen infrastructure that is often installed in individual equipment cabinets or ventilated enclosures. The ultimate objective of this effort is to develop guidance for an optimal sensor placement such that, when in-tegrated into a facility design and operation, will allow earlier detection at lower levels of incipient leaks, leading to significant hazard reduction. Reliable and consistent early warning of hydrogen leaks will allow for the risk mitigation by reducing or even elimi-nating the probability of escalation of small leaks into large and uncontrolled events. To address this issue, a study of a real-world mechanically ventilated enclosure containing GH2 equipment was conducted, where CFD modeling of the hydrogen dispersion (performed by AVT and UQTR, and independently by the JRC) was validated by the NREL Sensor laboratory using a Hydrogen Wide Area Monitor (HyWAM) consisting of a 10-point gas and temperature measurement analyzer. In the release test, helium was used as a hydrogen surrogate. Expansion of indoor releases to other larger facilities (including parking struc-tures, vehicle maintenance facilities and potentially tunnels) and incorporation into QRA tools, such as HyRAM is planned for Phase 2. It is anticipated that results of this work will be used to inform national and international standards such as NFPA 2 Hydrogen Technologies Code, Canadian Hydrogen Installation Code (CHIC) and relevant ISO/TC 197 and CEN documents. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved
Elisbirnd, Frank (Death, 1906-12-22)
No full textAddress: 2721 Buttner St.Age at death: 49 yrs.Pg 153/1906/404/M W M/City/Dr. A. R. Walker/J. Huth/St. John'sOriginal record filed in drawer labeled 'EISELE-EN'
Buttner, Dorothea (Death, 1877-07-24)
No full textAddress: Vine & Carthage PikeAge at death: 75 yrsPg 42/1877/386/F W M/Germany/Dr. Tuerk/J. Schreiber/Carthage Rd.Original record filed in drawer labeled 'BUSH-CAHILL'
Bixaceae Kunth, Malvac., Buttner., Tiliac.
No full text<p> 254. <b>Bixaceae</b> Kunth, <i>Malvac</i>., <i>Büttner</i>., <i>Tiliac</i>.: 17. 20 Apr 1822, nom. <i>cons</i>.</p> <p> <i>Cochlospermaceae</i> Planch, <i>London J</i>. <i>Bot</i>. 6: 305. Jun–Jul 1847, nom. <i>cons</i>.</p> <p> <i>Diegodendraceae</i> Capuron, <i>Adansonia</i>, ser. 2, 3: 392. Oct–Dec 1964</p>Published as part of <i>Reveal, James L., Chase, Mark W., Iii, - Apg & Iii, Apg, 2011, APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction, pp. 71-134 in Phytotaxa 19 (1)</i> on page 97, DOI: 10.11646/phytotaxa.19.1.4, <a href="http://zenodo.org/record/10121353">http://zenodo.org/record/10121353</a>
DevA, a GntR-like transcriptional regulator required for development in streptomyces coelicolor
No full textThe gram-positive filamentous bacterium Streptomyces coelicolor has a complex developmental cycle with three distinct phases: growth of the substrate mycelium, development of reproductive structures called aerial hyphae, and differentiation of these aerial filaments into long chains of exospores. During a transposon mutagenesis screen, we identified a novel gene (devA) required for proper development. The devA mutant produced only rare aerial hyphae, and those that were produced developed aberrant spore chains that were much shorter than wild-type chains and had misplaced septa. devA encodes a member of the GntR superfamily, a class of transcriptional regulators that typically respond to metabolite effector molecules. devA forms an operon with the downstream gene devB, which encodes a putative hydrolase that is also required for aerial mycelium formation on R5 medium. S1 nuclease protection analysis showed that transcription from the single devA promoter was temporally associated with vegetative growth, and enhanced green fluorescent protein transcriptional fusions showed that transcription was spatially confined to the substrate hyphae in the wild type. In contrast, devAB transcript levels were dramatically upregulated in a devA mutant and the devA promoter was also active in aerial hyphae and spores in this background, suggesting that DevA might negatively regulate its own production. This suggestion was confirmed by gel mobility shift assays that showed that DevA binds its own promoter region in vitro
Wolbachia endobacteria depletion by doxycycline as antiWlarial therapy has macroWlaricidal activity in onchocerciasis: a randomized placebo-controlled study
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