2,290 research outputs found
sj-pdf-1-phr-10.1177_00333549231170044 – Supplemental material for Development of a Standardized, Laboratory Result–Based Hepatitis C Virus Clearance Cascade for Public Health Jurisdictions
No full textSupplemental material, sj-pdf-1-phr-10.1177_00333549231170044 for Development of a Standardized, Laboratory Result–Based Hepatitis C Virus Clearance Cascade for Public Health Jurisdictions by Martha P. Montgomery, Lindsey Sizemore, Heather Wingate, William W. Thompson, Eyasu Teshale, Ade Osinubi, Mona Doshani, Noele Nelson, Neil Gupta and Carolyn Wester in Public Health Reports</p
Articulatory feature recognition using dynamic Bayesian networks
No full textWe describe a dynamic Bayesian network for articulatory feature recognition. The model is intended to be a component of a speech recognizer that avoids the problems of conventional ``beads-on-a-string'' phoneme-based models. We demonstrate that the model gives superior recognition of articulatory features from the speech signal compared with a stateof- the art neural network system. We also introduce a training algorithm that offers two major advances: it does not require time-aligned feature labels and it allows the model to learn a set of asynchronous feature changes in a data-driven manner
Meios de cultura e tamanhos de botões florais na indução à calogênese em anteras de Citrus webberi Wester cultivadas in vitro.
No full textObjetivo: O objetivo deste trabalho foi testar diferentes composições de meios de cultura na indução de calos a partir de anteras de Citrus webberi Wester
A naval travesty : the dismissal of Admiral Sir John Jellicoe, 1917
No full textThis dissertation relates to the dismissal of Admiral Jellicoe, First Sea Lord from November 1916 to December 1917, by Sir Eric Geddes, First Lord of the Admiralty, at the behest of the Prime Minister, David Lloyd George. The dismissal was peremptory and effected without rational explanation, despite Jellicoe having largely fulfilled his primary mission of combating the German U-boat threat to British merchant shipping. The outcome of the war may well have been affected if the level of shipping losses sustained through U-boat attack in April 1917 had continued unabated.
The central argument of the dissertation is that the dismissal was unjustified. As an adjunct, it argues that the received view of certain historians that Jellicoe was not successful as First Sea Lord is unwarranted and originates from severe post war critism of Jellicoe by those with a vested interest in justifying the dismissal, notably Lloyd George.
Supporting these arguments, the following assertions are made. Firstly, given the legacy Jellicoe inherited when joining the Admiralty, through the strategies adopted, organisational changes made and initiatives undertaken in anti-submarine weapons development, the progress made in countering the U-boat threat was notable. Secondly, the universal criticism directed at the Admiralty over the perceived delay in introducing a general convoy system for merchant shipping is not sustainable having regard to primary source documentation. Thirdly, incidents that occurred during the latter part of 1917, and suggested as being factors which contributed to the dismissal, can be discounted. Fourthly, Lloyd George conspired to involve General Haig, Commander of the British Forces France, and the press baron, Lord Northcliffe, in his efforts to mitigate any potential controversy that might result from Jellicoe’s removal from office. Finally, the arguments made by a number of commentators that the Admiralty performed better under Jellicoe’s successor, Admiral Wemyss, is misconceived
The longue duree of community engagement: New applications of critical theory in planning research
No full textHabermas' critical theory, and particularly his theory of communicative action, has been applied in the theory and practice of communicative planning. The concept of creating a public sphere in planning processes has been used as an ‘ought' that planners should seek to achieve to create a communicative rationality. Accepting some of the critique of communicative planning from an agonist and Foucauldian perspective, this paper presents a new application of Habermas' critical theory. Evidence is presented from community activists in two neighbourhoods of their ongoing reflection on the changes to their built environment over 20 years of regeneration. In this context, Habermas' theoretical work does explain the long-term discourse as the community moved towards a shared consensus on their neighbourhood. This is used to suggest that instead of looking for consensus in the tense conflicting of moments of initial engagement, planners should focus on the longue durée, and the Lifeworld of lived experience, where shared subjectivities over the built environment can develop
Income inequality, decentralisation, and regional development in Western Europe
No full textThis paper deals with the relationship between decentralisation, regional economic development, and income inequality within regions. Using multiplicative interaction models and regionally aggregated microeconomic data for more than 100,000 individuals in the European Union (EU), it addresses two main questions. First, whether fiscal and political decentralisation in Western Europe has an effect on within regional interpersonal inequality. Second, whether this potential relationship is mediated by the level of economic development of the region. The results of the analysis show that greater fiscal decentralisation is associated with lower interpersonal income inequality, but as regional income rises, further decentralisation is connected to a lower decrease in inequality. This finding is robust to the measurement and definition of income inequality, as well as to the weighting of the spatial units by their population size
Detergent extraction of herpes simplex virus type 1 glycoprotein D by zwitterionic and non-ionic detergents and purification by ion-exchange high-performance liquid chromatography
No full textDetergents (surfactants) are the key reagents in the extraction and purification of integral membrane proteins. Zwitterionic and non-ionic detergents were used for the extraction of recombinant glycoprotein D (gD-1) of herpes simplex virus type 1 (HSV-1) from insect cells infected with recombinant baculovirus. The highest yield was obtained with the two alkyl carboxybetaine detergents (N-dodecyl-N,N-dimethylammonio)undecanoate [DDMAU, critical micelle concentration (CMC)=0.13 mM] and (N-dodecyl-N,N-dimethylammonio)butyrate (DDMAB, CMC=4.3 mM). Therefore these zwitterionic detergents were used as additives to the elution buffers in ion-exchange high-performance liquid chromatography (HPIEC) to purify gD-1 of HSV-1 from the extracts. The non-ionic detergent pentaethyleneglycol monodecyl ether (C(10)E(5)) that was used in earlier studies [R.A. Damhof, M. Feijlbrief, S. Welling-Wester: G,W Welling, J. Chromatogr. A, 676 (1994) 43] was used for comparison. Two columns were used, Mono Q and Resource Q, at 1 and 5 ml/min flow-rates, respectively. The results show that the detergents DDMAU and C(10)E(5) are superior to DDMAB, when the detergents were used as additives to the elution buffers at 0.2% (w/v). With 0.2% DDMAB in the eluent, purification of HSV gD-1 was not possible. Detergents with a high CMC may be less suitable as additives in elution buffers. HPIEC at flow-rates of 1 and at 5 ml/min showed satisfactory results. At 5 ml/min HSV gD-1 was mainly concentrated in two eluent fractions. The highest recovery of gD-1 was obtained either by chromatography of a C(10)E(5) extract using a Mono Q column at a flow-rate of 1 ml/min or by chromatography of a DDMAU extract using a Resource Q column at a flow-rate of 5 ml/min. (C) 1998 Elsevier Science B.V. All rights reserved
Detergent extraction of herpes simplex virus type 1 glycoprotein D by zwitterionic and non-ionic detergents and purification by ion-exchange high-performance liquid chromatography
No full textDetergents (surfactants) are the key reagents in the extraction and purification of integral membrane proteins. Zwitterionic and non-ionic detergents were used for the extraction of recombinant glycoprotein D (gD-1) of herpes simplex virus type 1 (HSV-1) from insect cells infected with recombinant baculovirus. The highest yield was obtained with the two alkyl carboxybetaine detergents (N-dodecyl-N,N-dimethylammonio)undecanoate [DDMAU, critical micelle concentration (CMC)=0.13 mM] and (N-dodecyl-N,N-dimethylammonio)butyrate (DDMAB, CMC=4.3 mM). Therefore these zwitterionic detergents were used as additives to the elution buffers in ion-exchange high-performance liquid chromatography (HPIEC) to purify gD-1 of HSV-1 from the extracts. The non-ionic detergent pentaethyleneglycol monodecyl ether (C(10)E(5)) that was used in earlier studies [R.A. Damhof, M. Feijlbrief, S. Welling-Wester: G,W Welling, J. Chromatogr. A, 676 (1994) 43] was used for comparison. Two columns were used, Mono Q and Resource Q, at 1 and 5 ml/min flow-rates, respectively. The results show that the detergents DDMAU and C(10)E(5) are superior to DDMAB, when the detergents were used as additives to the elution buffers at 0.2% (w/v). With 0.2% DDMAB in the eluent, purification of HSV gD-1 was not possible. Detergents with a high CMC may be less suitable as additives in elution buffers. HPIEC at flow-rates of 1 and at 5 ml/min showed satisfactory results. At 5 ml/min HSV gD-1 was mainly concentrated in two eluent fractions. The highest recovery of gD-1 was obtained either by chromatography of a C(10)E(5) extract using a Mono Q column at a flow-rate of 1 ml/min or by chromatography of a DDMAU extract using a Resource Q column at a flow-rate of 5 ml/min. (C) 1998 Elsevier Science B.V. All rights reserved.</p
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