3,945 research outputs found
Pharmacological read-through of nonsense ARSB mutations as a potential therapeutic approach for mucopolysaccharidosis VI.
No full textLIGHT-DARK CYCLE AND MITOTIC INDEX IN ASELLUS-AQUATICUS (L) (CRUSTACEA, ISOPODA)
No full textThe circadian variation of the mitotic index during spermiohistogenesis was studied in Asellus aquaticus (L.). The actual number of metaphases and prometaphases was determined at the end of each hour of light or darkness over a 24 h period in animals bred under LD 12:12. The number of the metaphases and prometaphases decreases during the light period and sharply increases in the last 3 hrs of the dark period. This variation in the proliferative activity suggests that photoperiod can play a role in the synchronization of mitosis
Fluorescence in situ hybridization (FISH) for genomic investigations in rat.
No full textThis chapter concentrates on the use of fluorescence in situ hybridization (FISH) for genomic investigations in the laboratory rat (Rattus norvegicus). The selection of protocols included in the chapter has been inspired by a comprehensive range of previously published molecular cytogenetic studies on this model organism, reporting examples of how FISH can be applied for diverse investigative purposes, varying from comparative gene mapping to studies of chromosome structure and genome evolution, to characterization of chromosomes aberrations as well as transgenic insertions. The protocols, which include techniques for the preparation of mitotic chromosomes and DNA fibers from short-term cell cultures, have been gathered through the years and repeatedly tested in our laboratory, and all together aim at providing sufficient experimental versatility to cover a broad range of cytogenetic and cytogenomic applications
Aggregated Impact of EV Charger Type and EV Penetration level in Improving PV Integration in Distribution Grids
No full textMass deployment of Electric Vehicles (EVs) can improve the loading characteristics of low voltage distribution grids with high Photovoltaic (PV) penetration. This impact is investigated in the paper from two point of views, namely, the EV charger type and the EV penetration level. Based on the measured usage data for home, public and semi-public EV chargers, it is highlighted that the ratio of the number of these charger types can influence the grid level impact of PV penetration. Using Monte-Carlo method with aggregated power balance model, it is suggested that the increase in percentage of public and semi-public chargers relative to home chargers can improve self-consumption of PV energy in the grid, thereby reducing the power mismatch due to excess local generation. A PowerFactory based simulation with real measurement based data on real German distribution grids reveals that the grids have no risk of congestion at all with 80% EV penetration, allowing for a possibility even higher EV penetration in the future. Furthermore, with the considered uncontrolled EV charging, it is observed that the grids experience reverse power flows due to excess PV generation. This excess PV energy reduces by about 5% with high EV penetration, indicating a future potential for targeted smart charging application for improving these benchmarked results.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.DC systems, Energy conversion & Storag
Differentially expressed genes selected on spline (EV) or VSN transformed data
No full text<p><b>Copyright information:</b></p><p>Taken from "An adaptation of the LMS method to determine expression variations in profiling data"</p><p></p><p>Nucleic Acids Research 2007;35(9):e71-e71.</p><p>Published online 25 Apr 2007</p><p>PMCID:PMC1888829.</p><p>© 2007 The Author(s)</p> The same dataset represented in was used to select differentially expressed genes on spline (EV) or VSN-transformed data. MA plots of scaled log-transformed signals for probe sets from two experimental conditions of the B6.1 cells. Color codes are as in . Up-regulated genes are shown as green bold dots, down-regulated as red bold dots. () Distribution of 555 genes (-value = 0.05) selected upon a spline fit. () Distribution of 555 genes (FC = 1.63) on VSN-transformed data. () Distribution of genes selected by both approaches. () Distribution of genes selected by EV (spline fit) alone. () genes selected by VSN (arcsinh transformation) alone
Numerical abnormalities of chromosomes 1 and 10 in endometrial adenocarcinoma
No full textRetrospective analysis of chromosomal changes in endometrial carcinoma was performed by fluorescence in situ hybridization on free nuclei isolated from formalin-fixed paraffin-embedded tissue. We examined 23 archival samples for numerical aberrations of chromosomes 1 and 10 with the use of specific DNA probes for the pericentromeric and centromeric regions of these two chromosomes. Numerical aberrations of chromosomes 1 and 10 were detected in 39% of the case analyzed, and the frequency of trisomy 10 tended to increase as the histological grade worsened. Our findings confirm the association of cytogenetic anomalies involving chromosomes 1 and 10 with endometrial carcinoma, as reported by other studies, and suggest that changes in centromere 10 copy number may correlate with the degree of tumor differentiation
Frequency Regulation Reserves Provision in EV Smart-Charging
No full textSmart-Charging of Electric Vehicles (EVs) is able to provide frequency regulation capacity services to the System Operator (SO) upon an automation generation control (AGC) signal. While the amount of available regulation capacity is of-fered in the Day-Ahead Market (DAM), there is high uncertainty on the actual amount of reserves that will be called in the Real-Time Market (RTM). This work focuses on aiding EV smart-charging to offer a consistent and reasonable amount of regulation capacity, taking into account the impact of potential future instantaneous called regulation reserves while also maintaining simplicity. The work also analyzes the results of different charger types with different characteristics and shows that they play an important role on the regulation provision. Finally, it has been shown that even though the regulation income is inevitably reduced (up to 66%), the Energy Management System (EMS) can still successfully charge the EV s and simultaneously provide regulation reserves with remuneration.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.DC systems, Energy conversion & Storag
Optimal System Design for a Solar Powered EV Charging Station
No full textCharging electric vehicles (EV) from photovoltaic (PV) panels provides a sustainable mode of transportation. In order to reduce the net costs of charging EV from PV and the grid, the PV generation and/or the EV charging can be controlled based on the energy prices in the grid. The traditional approach to designing the solar system for EV charging is to maximize the energy yield. In this paper, an alternate approach to PV system design is proposed by which the PV panels are orientated so as to maximize the PV revenue. This technique is compared with that of reducing the net costs by smart charging of the EV based on energy prices. Two case studies for Netherlands and Texas are done to compare the PV energy generated and the net cost of EV charging from PV based on the two techniques.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.DC systems, Energy conversion & Storag
End-of-Life Comparison of Full-Bridge and Half-Bridge DC/DC Converter Switches Used for EV Charging
No full textEV fast chargers are essential in addressing the concern of limited driving range for E-mobility applications. However, the load profile of a converter for fast charging involves a high-current pulse that can last for a few minutes to efficiently replenish the EV battery, which is followed by a cooldown period after the charging process is finished. This results in thermal cycles that can lead to thermo-mechanical fatigue and degradation of power electronic components, thereby impacting device lifetime. This paper presents a comparative study on the reliability of power devices in isolated half-bridge and full-bridge DC-DC converters in EV fast chargers. The study focuses on the differences in thermal stresses that Si switches experience in each converter during charging cycles and how it impacts the end-of-life of each device. This study provides valuable insights for selecting reliable power converters for EV fast charging applications.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.DC systems, Energy conversion & Storag
Using dedicated EV charging areas to resolve grid violations caused by renewable energy generation
No full textEV Charging is expected to be a significant load on the grid in the future. But they can also be a part of the solution to grid violations. As the amount of renewable energy generation increases, there is a large amount of power flowing in the reverse direction in the distribution network, which is undesirable. To reduce this, EVs can charge at strategically located “Grid Management Parking Lots”. Here the EV charging power level varies in accordance with the amount of capacity available in the grid. This is a win-win situation for both parties, the grid operator and the EV owner, as the grid operator reduces the amount of violations in the grid and the EV owner has his/her vehicle charged sooner.Accepted Author ManuscriptDC systems, Energy conversion & Storag
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