150 research outputs found
Actividad antibacteriana de extractos de hojas de: mentha vindis cordia lutea. Tagetes minuta parkinsonia aculeata y cappris scabrida
No full textActividad antibacteriana de extractos de hojas de: mentha vindis cordia lutea. Tagetes minuta parkinsonia aculeata y cappris scabridaTesi
Research & Development Project GIFT AR (DATUM INNOVATE UK) and public output DONATE YOURSELF (HUMAN CELL ATLAS)
Get PDFAs our lives become increasingly virtual AR GIFT (DATUM) offers the opportunity to bring depth to our connected relationships, across time and space.
Whether a quick thank you or a special present this platform enables fast and beautiful gift giving within the blended environment of AR (physical and virtual), enabling action on environmental sustainability and yet enhancing and extending the unique intimacy of gifting.
AR GIFT (DATUM INNOVATE UK) is a mobile app/web-based gifting platform enabling the user to choose, customise and send an AR gift to a friend/colleague, wherever they are in the world, eliminating unsustainable physical gifting practises.
These virtual gifts exist on the platform as AR assets, both ready-made and customisable, which are sent by the gift giver and collected by the recipient at a time and location of the gift givers choice. Each bespoke choice by the gift giver is an intimate act in itself.
The platform is responding to the need for intimate gifting (thank yous, get well wishes, birthday greetings or condolences) into our connected world, local to local and local to global, brought to the forefront in our times of global pandemic lockdowns, isolation and quarantine.
It is in direct response to the growing need, through social distancing, for deeper remote intimacy interactions, and the post-lockdown requirements for collective and intimate experiences that are conducted in a safe and responsible manner. While at the same time, this platform encourages ethical consumer behaviour and runs alongside the re-opening of sustainable commerce post pandemic.
AR GIFT Team - body>data>space (BDS Creative Ltd)
Ghislaine Boddington - Creative Director
Tadej Vindis - Lead Producer
Nick Rothwell - Technical Lead
Ivor Diosi - XR Developer
The first commission output for AR GIFT is Donate Yourself, an Augmented Reality experience co-created by artist Stacey Pitsillides with Ghislaine Boddington and the body>data>space collective. It blends sound and 3D visuals to spark debates about our organs, tissue and body data, accessed by the public through augmented reality via QR codes.
This new work was encountered in several ways. It premiered as a walking tour around the Ouseburn Valley area of Newcastle Upon Tyne, UK (29th October – 30th November). There, on this trail of five locations, you found the Donate Yourself banners and, by scanning the QR codes on these banners with your mobile phone and listening to the audio stories, you will take part in an inspirational journey. The work also was shown in Oxford, London and Cambridge across November / December 2021 and is still touring.
The digital objects that you encounter will be seen through your phone imposed on the landscapes behind and, with the audio in your ears, stories of care, trust, immortality, consent and futures will unfold, exploring the important role our bodies play in scientific discovery.
Each experience questions how we see our body after death; as a collective source of knowledge for humanity, as a material to explore our biological make up, or even as a way of immortalising ourselves in cells.
These AR sound and visual objects examine diverse perspectives on what donating parts of yourself mean to different people. See lungs breathing posthumous digital data, view eyes blooming up above us and neurons radiating from a petri dish, hear the unfolding audio stories as you walk, imposed on the real world around you.
What role can our bodies play in scientific discovery?
Could we see ourselves as a collection of cells?
Does donating organs or tissue make you immortal?
This sci-art project shares artistic interpretations of scientific imagery with the audience, from interviews with experts from the Human Cell Atlas research initiative and visual/written data from a series of artists workshops which are expanded through this unique digital experience. Gathered from a range of communities Stacey Pitsillides and body>data>space created this AR experience to help us all consider the legacy of our bodies in this digital age.
AR GIFT (DATUM) is created and produced by body>data>space (BDS Creative Ltd). The project was developed as part of DATUM R&D, an Innovate UK funded project with partners ZU-UK (Lead Partner), body>data>space and University of Greenwich (Innovate UK Sustainable Innovation Fund: Round 1 (Temporary Framework) 2020), and in further partnership with CLEI Co-creating Liveness in Embodied Immersion, BHRE Business, Human Rights and Environment, and LETS Law, Emerging Technologies & Science Strategic Research Groups at University of Greenwich.
Donate Yourself - co-created by Stacey Pitsillides with body>data>space (2021). A One Cell at a Time commission with the Human Cell Atlas research initiative:
The digital AR experience has been designed and co-created between Stacey Pitsillides and the body>data>space team using the b>d>s AR GIFT development project: Ghislaine Boddington (Creative Co-Direction), Tadej Vindis (Project Development and Production), Nick Rothwell (Sound Design and Technical Development) and Ivor Diosi (AR Development and 3D Animation). With research and insights from Holly Standing and Luke Sellers. Donate Yourself is produced for One Cell at a Time by Dominic Smith.
Donate Yourself is created as part of the AR GIFT development project at body>data>space, supported by Innovate UK and the University of Greenwich (2021-22)
EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis.
No full textEph receptors and their ligands (ephrins) play an important role in axonal guidance, topographic mapping, and angiogenesis. The signaling pathways mediating these activities are starting to emerge and are highly cell- and receptor-type specific. Here we demonstrate that activated EphB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. EphB1-mediated increase of cell migration was abrogated by the MEK inhibitor PD98059 and Src inhibitor PP2. In contrast, cell adhesion, which we previously showed to be c-jun NH2-terminal kinase (JNK) dependent, was unaffected by ERK1/2 and Src inhibition. Expression of dominant-negative c-Src significantly reduced EphB1-dependent ERK1/2 activation and chemotaxis. Site-directed mutagenesis experiments demonstrate that tyrosines 600 and 778 of EphB1 are required for its interaction with c-Src and p52Shc. Furthermore, phosphorylation of p52Shc by c-Src is essential for its recruitment to EphB1 signaling complexes through its phosphotyrosine binding domain. Together these findings highlight a new aspect of EphB1 signaling, whereby the concerted action of c-Src and p52Shc activates MAPK/ERK and regulates events involved in cell motility
Multicomponent Output: Gift AR platform, Nudge and a Push webinar series, Donate Yourself AR artwork and article
No full text- The AR GIFT (part of INNOVATE UK DATUM) - a mobile app/web-based gifting platform enabling the user to choose, customise and send an AR gift to a friend/colleague, wherever they are in the world, eliminating unsustainable physical gifting practises.These virtual gifts exist on the platform as AR assets, both ready-made and customisable, which are sent by the gift giver and collected by the recipient at a time and location of the gift givers choice. Each bespoke choice by the gift giver is an intimate act in itself. The platform responds to the need for intimate gifting (thank yous, get well wishes, birthday greetings or condolences) into our connected world, local to local and local to global, brought to the forefront in our times of global pandemic lockdowns, isolation and quarantine. It is in direct response to the growing need, through social distancing, for deeper remote intimacy interactions, and the post-lockdown requirements for collective and intimate experiences that are conducted in a safe and responsible manner. While at the same time, this platform encourages ethical consumer behaviour and runs alongside the re-opening of sustainable commerce post pandemic.
- Additionally Boddington curated and moderated a series of 4 future-looking public (live webinar) talks emerging from creators of similar participatory, immersive and interactive experiences. This debate series "A nudge and a push: towards ethical sustainability in an era of (invisible) data harvesting" was streamed live by University of Greenwich during lockdown (2021) and examined topical issues for the VR, AR, XR, immersion, arts and gaming sectors in terms of sustainable and ethical processes, exploring approaches to the production and usage of such outputs for public education and engagement. Speakers were curated from a wide range of international academic, creative industries and arts sectors. The 4 webinars had attendance of over 500 people and led to ongoing debates, research, touring opportunities and partnerships.
- The archive recordings are published on the University of Greenwich Research Space You Tube site as a playlist with educational resource documents for teachers and students created and attached to each webinar. (828 views to end of 2024)
- The first commission output for AR GIFT was Donate Yourself, an Augmented Reality experience co-created by artist Stacey Pitsillides with Ghislaine Boddington and the body>data>space collective. It blends sound and 3D visuals to spark debates about our organs, tissue and body data, accessed by the public through augmented reality via QR codes.The Donate Yourself AR touring artwork was created as part of the AR GIFT development project at body>data>space, supported by Innovate UK and the University of Greenwich (2021-22). Five themes were generated for the augmented reality experiences: care, trust, immortal, consent, future. This triangulation of science, technology and the arts was materialised in a webAR walking trail that ran during November 2021, is still touring internationally and which is accessed by the public via QR codes as part of the larger One Cell At A Time online exhibition, funded by the Wellcome Trust.
- Finally an article about this practice led research for social impact was published exploring how communication and interaction design were used in the augmented reality experience, Donate Yourself. It aims to demystify some of the ethical and personal concerns around the donation of organs, tissue and body data for scientific and medical research. (Pitsillides, Stacey, Boddington, Ghislaine ORCID logo and Vindis, Tadej (2022) Donate yourself: an AR trail exploring the future of organ, tissue and body data donation. Virtual Creativity, 12 (1). pp. 103-123. ISSN 2397-9704 (Print), 2397-9712 (Online) (doi:10.1386/vcr_00064_1
Role of mitophagy in physiopathology of atherosclerosis
No full textL'athérosclérose est une pathologie progressive, à évolution lente qui consiste en la formation d'une plaque d'athérome dans la paroi artérielle dont la rupture est à l'origine de complications athéro-thrombotiques graves. La nature et le devenir des lésions d'athérosclérose dépendent en grande partie de l'équilibre entre la survie et la mort des composants cellulaires de la plaque. Les cellules musculaires lisses (CML) qui composent la chape fibreuse sont essentielles à la stabilité de la plaque, ainsi l'apoptose des CML contribue à la fragilisation et à la rupture de la plaque. Dans des conditions de stress ou de carence nutritive les cellules activent des voies d'adaptation et de survie comme l'autophagie, un processus d'autodigestion du matériel cellulaire par la voie de dégradation lysosomale. De part sa participation à la régulation de fonctions cellulaires, une dérégulation de l'autophagie est aussi évoquée dans les maladies cardiovasculaires. Bien que l'autophagie soit un processus non sélectif, elle peut aussi éliminer sélectivement des organites tels que les mitochondries. La dégradation sélective des mitochondries ou mitophagie participe ainsi au contrôle qualité des mitochondries, mais permet aussi de prévenir l'apoptose dans des conditions pathologiques où les mitochondries sont altérées. Ainsi dans conditions de stress métabolique et oxydant la mitophagie pourrait limiter l'augmentation des ERO mitochondriaux et la libération de protéines pro-apoptotiques dans le cytosol, afin de protéger les CML de la chape fibreuse contre la mort cellulaire. L'objective de mon projet de thèse à été de caractériser et d'étudier le rôle de la mitophagie dans les CML vasculaires soumises à un stress athérogène. Nos résultats ont permis de mettre en évidence pour la première fois que la mitophagie est activée dans les CML humaines en présence de lipides athérogènes oxydés. Nous avons identifié des dysfonctions mitochondriales (altération du potentiel de membrane, fission) et la voie de signalisation Drp1/PINK1/Parkin comme impliquées dans la mise en place de la mitophagie. La quantification du flux mitophagique basal et dans les conditions d'invalidation ou de surexpression de PINK1 et Parkin dans les CML humaines nous ont permis de démontrer que la mitophagie est un mécanisme de défense contre l'apoptose induite par un stress lipidique athérogène. Dans une deuxième partie, nous avons généré un modèle murin d'athérosclérose possédant une délétion spécifique du gène Atg7 dans les CML. L'analyse du phénotype des plaques montre que la déficience en autophagie conduit au développement d'une plaque instable avec une augmentation de l'apoptose et de l'inflammation. L'analyse des paramètres de la mitophagie montre une dysfonction de ce processus à l'origine d'une altération du contrôle qualité mitochondrial avec une augmentation de la fragmentation et de la production d'ERO mitochondriaux. Ces résultats suggèrent qu'une altération du processus d'auto/mitophagie peut contribuer au développement de plaques d'athérosclérose instables. En conclusion, nos résultats renforcent l'intérêt de la mitophagie comme un nouveau marqueur de fragilisation de la plaque et une cible potentielle pour de nouvelles stratégies thérapeutiques visant à stabiliser la plaque d'athérome.Atherosclerosis is a progressive vascular disease, resulting from deposition of lipids in the arterial wall and subsequent plaque formation. Plaque destabilization and rupture causes rupture serious atherotrombotic complications such as myocardial infarction, stroke or sudden cardiac death. The mechanisms involved in plaque destabilization and rupture are rather complex and depend, at least in part, on the survival versus death balance of the cellular components of the lesion. The vascular smooth muscle cells (VSMCs) comprising the fibrous cap of the plaque are essential for its stability. Thus VSMCs apoptosis can lead to increased plaque fragility and rupture. During nutrient deprivation or stress conditions, the cells activate cell safeguard mechanisms such as a autophagy - a conserved lysosomal process of intracellular organelle degradation. Because of its crucial role in the regulation of cell homeostasis, deregulation of autophagy is implicated in development of different pathologies such as cardiovascular diseases. However autophagy is not only a nonselective process, but can instead target selectively organelles such as mitochondria. The selective mitochondrial degradation or mitophagy is implicated both in mitochondrial quality-control and in the prevention of apoptosis in different pathologic conditions where mitochondria are altered. Thereby the mitophagy could limit the increase of mitochondrial reactive oxygen species (ROS) production and the release of pro-apoptotic proteins in the cytosol during metabolic or oxidative stress, and prevent the death of the VSMC of the fibrous cap. The objective of my research work was to characterize mitophagy and to identify its role in VSMC exposed to the atherogenic stress. We have established for the first time that in response to atherogenic stressors human VSMCs activated mitophagy. We described mitochondrial dysfunction (membrane potential alteration and fission) and the signaling pathway Drp1/PINK1/Parkin as implicated in mitophagy initiation. The quantification of mitophagy flux in baseline and PINK1 or Parkin knockdown or overexpression conditions in human VSMC allowed us to demonstrate that the mitophagy is a safeguard mechanism against apoptosis induced by an atherogenic lipid stress. Next we generated a mouse model of atherosclerosis harboring or not VSMC specific deletion of autophagy gene Atg7. The plaque phenotype analysis demonstrates that autophagy deficiency led to the destabilization of the plaque characterized by increased apoptosis and inflammation. The mitophagy parameter analysis demonstrated the dysfunction of this process leading to de alteration of mitochondrial quality-control with an increase of fragmentation and mitochondrial ROS production. These results suggest that the alteration of auto-/mitophagy can contribute to the unstable atherosclerotic plaque development. In conclusion, our results emphasize the importance of mitophagy as a new plaque instability marker and a potential target for development of new strategies aimed at stabilizing the atheroma plaque
EphB1-mediated cell migration requires the phosphorylation of paxillin at Tyr-31/Tyr-118.
No full textInteractions between Eph receptors and their membrane-bound ligands (ephrins) are of critical importance for key developmental processes such as boundary formation or vascular development. Their downstream signaling pathways are intricate and heterogeneous at several levels, the combined effect being a highly complex and flexible system. Here we demonstrate that activated EphB1 induces tyrosine phosphorylation of the focal adhesion protein paxillin at Tyr-31 and Tyr-118 and is recruited to paxillin-focal adhesion kinase (FAK) complexes. Pretreatment with the specific Src inhibitor PP2, or expression of dominant-negative, kinase-dead c-Src abrogates EphB1-induced tyrosine phosphorylation of paxillin. Cells transfected with the paxillin mutant Y31F/Y118F displayed a reduced migration in response to ephrin B2 stimulation. Furthermore, expression of an LD4 deletion mutant (paxillin DeltaLD4) significantly reduces EphB1-paxillin association, paxillin tyrosine phosphorylation, as well as EphB1-dependent cell migration. Finally, mutation of the Nck-binding site of EphB1 (Y594F) interrupts the interaction between Nck, paxillin, and EphB1. These data suggest a model in which ligand-activated EphB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration
Digital Human Twins: Our Future Data Selves 2021 – 2027. MY AI HYBRID BIOTWIN: a series of keynotes, radio interviews, lectures, panels and presentations around key research focus
Get PDFAcross March 23 to June 24 I prepared and delivered a series of keynotes, radio interviews, lectures, podcasts, panels and presentations using topical materials from my key research theme My AI HYBRID BIOTWIN (part of my long term The Internet of Bodies research umbrella). These invited outputs are linked to my practice-led research into Digital Human Twins - our future data selves. This foresight work has gained a lot of interest. This is due to knowledge of my long term expertise and the high interest in digital twins in a wide variety of sectors. I work with my original content and foresight to translate and facilitate this research and bridge it into knowledge exchange. The aim is to engage a wider public alongside specialists in the field. This supports interdisciplinary and cross sector exchange on the evolution of new forms of virtual representation of the self, creating embodied insights for individuals into the future of the virtual physical blended body, liveness, intimacy and enabling debates about the positives and negatives (ethics) of the integration of AI and biometrics into future body technologies. My aim is to expand knowledge and enabling such discussions as understanding these shifts for the living body in relationship to technological advancements is important for us all. The audiences ranged from radio and podcast listeners (global) , to academics, creative industry experts, artists, students, festival goers, creative industry communities and policy makers (UK and EU). These outputs impact into public discourse on a variety of levels, into arts and cultural sectors, creative industry and corporate sectors and, through the media reach and podcast access, into a global public. I am also maintaining a close adherence to diversity and inclusivity as I explore the topic of future identities.
Digital Human Twins - our future data selves is a practice-as-research initiative led by Ghislaine Boddington (2021-27), exploring and developing a personalised digital human twin, an AI hybrid biotwin, a digital counterpart that grows, learns, and evolves alongside their human partners, that supports and guidance from birth and beyond death. These digital twins are conceived to offer a unique blend of technological innovation and human experience, linked to AI and real-time biometrics. They push the boundaries of personal and professional development, health monitoring and identity management, while extending human presence far beyond physical limitations.
Please see more information and links in attached PDF.
- BOLD Unconference Austria (12-17 June 2024 Austria) Invited participant, presenter, panel moderator on digital human twins (MY AI HYBRID BIOTWIN). BOLD is the flagship event of the BOLD Community (Austrian Federal Economic Chamber). This Unconference brought together over 50 global visionaries from 27 countries involved in innovative activities in a range of sectors including business, research, policy-making, and the creative industries.
- Plexal, Here East (creative tech hub) - Cyber Lates (12 Dec 2023) Panel member “How to have a better relationship with the internet, take control of privacy, and protect ourselves from online harms”. Creative industry community.
- Frequency International Festival of Digital Culture and University of Lincoln - The Meet Up AI + Exploration: Innovation and Inclusion (27 Oct 2023) Research, preparation and moderation of a roundtable discussion. I bought together a panel of academics and artists to explore how rapid innovation in AI is enabling them to explore and challenge the parameters of practice, inclusion and engagement through experimentation and engagement with audiences and visitors. The opportunity these technologies present to nurture inclusion whilst navigating the ethical challenges and bias was discussed.
- Hybrid Presence Round Table 2 - What is next for DHT Digital Human Twins? (19 Oct 2023) Ghislaine explored the near future potential of “owning and nurturing” our own AI Hybrid BioTwin.
- BBC Radio 4 - "How to Spot Potential" (18 Oct 2023) - invited expert on future skills for young people linked to innovative digital tools, including AI companions for education and work searches. Ghislaine Boddington discuses the concept of digital twins, our very own personalised form of AI.
- The Navigator Podcast - University of Greenwich Generator (3rd Oct 2023) "Mind over Machine' - participant in round table debate on the ethical implications and yet vast potential of using AI in the future and how it will affect both education and the wider world
- AACSB (Association to Advance Collegiate Schools of Business) Online Learning Affinity Group (OLAG) (21 Sept 2023) lecture on the future student and learning / teaching shifts in next decade linked to research project MY AI HYBRID BIOTWIN - University of Greenwich Business School.
- Synthetic Syntax Conference, Ars Electronica - Keynote Speaker (10 Sept 2023) This fourth edition of the symposium Synaesthetic Syntax presented different perspectives on haptic bodies, their impact, liveliness and procedurality in the context of performance and concluded with a keynote lecture by Ghislaine Boddington.
- Tomorrow Unlocked Fast Forward Series 2. (June to August 23)
Ep. 4 "Extended self - our future digital twins": Uncover the transformative power of personalised body data and the boundless possibilities it offers. What about the lack of diversity of the data available? Guests: Michael D. Geer, Co-Founder of Humanity Inc. and Marija Butkovic, Founder of Women of Wearables (wearables, health tech & femtech)
Ep. 5 - "Cyborg Shifts – embedding technologies into our bodies": Discover groundbreaking advancements in cyborg technology, as prostheses and implants converge, creating opportunities for shifts towards cyborg existences. Guests: Dani Clode, University of Cambridge and trans-species artist Neil Harbisson. I was commissioned to research, script and present these podcasts as two of a series of six.
- BBC Radio 3 - "Free Thinking" (7 June 2023) - invited expert to talk about digital human twins
- The Brains Lab Launch, Kingston University – Keynote Speaker (22 May 2023) on "The Internet of Bodies - alive, connected and collective' with final section on digital human twins.
- BBC Radio 4 - "Super Senses" (9 May 2023) invited expert to talk about touch and intimacy in digital age in relationship to virtual bodies.
- BBC World Service Digital Planet (8 March 2023). “Digital identity: Where are we now?” exploring and overviewing the biometric led digital identity systems fast emerging in various continents – from China to the US and India and Europe. Show researched, scripted and co-presented by Ghislaine Boddington
Recommended from our members
Building trust - from television to the internet : crowds, trust and digital engagement
Get PDFIn the late 1960s, American democracy entered a crisis of trust and declining civic engagement. Policy makers interested in rebuilding trust with the people have often resorted to new communication technologies, but the impact of these technologies is sometimes unclear. The research presented here found that the impact of novel communication technologies is sometimes over-rated and that too much significance has been placed on leadership charisma. Sound strategic and tactical plans are critical to a successful outcome of any trust building activity, as trust is not necessarily created and sustained through the same means. Second, such plans must regularly be revised, and require competent expert support to execute strategic goals. This work concludes that – at all levels of public leadership – a change in thinking about digital technology is required, and that such technologies should be approached as opportunities rather than threats. Such an approach helps identify and deploy creative solutions, and speaks to younger generations who are more likely to embrace new technologies.Public Polic
Autophagy in health and disease: focus on the cardiovascular system
No full textAutophagy is a highly conserved mechanism of lysosome-mediated protein and organelle degradation that plays a crucial role in maintaining cellular homeostasis. In the last few years, specific functions for autophagy have been identified in many tissues and organs. In the cardiovascular system, autophagy appears to be essential to heart and vessel homeostasis and function; however defective or excessive autophagy activity seems to contribute to major cardiovascular disorders including heart failure (HF) or atherosclerosis. Here, we review the current knowledge on the role of cardiovascular autophagy in physiological and pathophysiological conditions.</jats:p
- …
