4,330 research outputs found
5.07.016: Over the Sea by Glenn Vincent Breen, 1998
No full textCD case insert for Glenn Vincent Breen's Over the Sea, 1998
Docosahexaenoic acid inhibits the invasion of MDA-MB-231 breast cancer cells through upregulation of cytokeratin-1
No full textDocosahexaenoic acid (DHA), the main member of the omega-3 essential fatty acid family, has been shown to reduce the invasion of the triple-negative breast cancer cell line MDA-MB-231, but the mechanism involved remains unclear. In the present study, a proteomic approach was used to define changes in protein expression induced by DHA. Proteins from crude membrane preparations of MDA-MB-231 cells treated with 100 µM DHA were separated by two-dimensional electrophoresis (2-DE) and differentially expressed proteins were identified using MALDI-TOF mass spectrometry. The main changes observed were the upregulation of Keratin, type II cytoskeletal 1 (KRT1), catalase and lamin-A/C. Immunocytochemistry analyses confirmed the increase in KRT1 induced by DHA. Furthermore, in vitro invasion assays showed that siRNA against KRT1 was able to reverse the DHA-induced inhibition of breast cancer cell invasion. In conclusion, KRT1 is involved in the anti-invasive activity of DHA in breast cancer cells
Imaging the lifecycle of Microsporidia sp. MB in Anopheles coluzzii from western Burkina Faso reveals octosporogony
No full textA newly discovered microsporidian, Microsporidia sp. MB (MB), was recently found to block Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes from Kenya. Here, we describe the lifecycle of the first laboratory colony of Anopheles coluzzii with MB, originating from western Burkina Faso. The lifecycle of MB was explored using fluorescent in situ hybridization and confocal microscopy, facilitated by the development of optimized protocols to produce histological sections of whole adult, larval, and embryo tissues. As in An. arabiensis, transmission appears to be predominantly vertical, with MB highly localized to the ovaries across multiple lifecycle stages. MB was sparsely distributed within the majority of developing oocytes in the gravid female. After oviposition, in the majority of embryos, MB relocated to the developing gonad at the onset of tissue differentiation, suggesting a highly specialized adaptation to host tissues. Sporogony was identified for the first time in a proportion of developing oocytes and in embryos post-oviposition. Microsporidian spore characteristics were subsequently confirmed with electron microscopy. Identification of MB sporogony in eggs suggests there are alternative horizontal routes of transmission which could play an important role in developing MB as a malaria control strategy
Front and Side Façade of St. Vincent Hotel, 100 North Wind Street, Flandreau SD, Moody County
No full text35 mm slide, a two-story building with window awnings, a two-story addition and a covered porch supported by turned post columns, the building bears the text "St. Vincent Hotel Since 1897", a sign extending from the building bears the text "Hotel", signs in the foreground bears the text "Kone King Special Chili Dog & Sm Pop $1.00" and "Open to Regular 1.189 Self Serve"Drawer info: Minnehaha - Pennington; St. Vincent HotelKodachrome Slide Moody Co. Flandreau St. Vincent Hotel MB 21 Nov 84C1
Receiver Windowing Design for Narrowband Interference Mitigation in MB-OFDM UWB System
No full textIn 2005, the WiMedia Alliance working with the European Computer Manufacturers Association (ECMA) announced the establishment of the WiMedia MB-OFDM (Multiband Orthogonal Frequency Division Multiplexing) UWB radio platform as their global UWB standard. It was also chosen as the physical layer (PHY) of high data rate wireless specifications for high speed Wireless USB (W-USB), Bluetooth 3.0 and Wireless High-Definition Media Interface (HDMI). However, due to the low power and wide bandwidth nature of UWB systems, in-band narrowband interference (NBI) may hinder the receiver performance. This thesis presents an analysis of NBI impact on the MB-OFDM system for UWB communication. The intent of our analysis is to provide practical solutions for interference mitigation under different NBI models. In our work, a new receiver windowing for zero padding (ZP) OFDM system is proposed to reduce NBI spreading in the MB-OFDM UWB system. Simulations demonstrate the effectiveness of windowing under different NBI models.Microelectronics & Computer EngineeringElectrical Engineering, Mathematics and Computer Scienc
Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells
No full textIntroduction After the onset of bone metastasis, tumor cells appear to modify surrounding microenvironments for their benefit, and particularly, the levels of circulating fibroblast growth factor (FGF) 23 in patients with tumors have been highlighted. Materials and methods We have attempted to verify if human breast carcinoma MDA-MB-231 cells metastasized in the long bone of nu/nu mice would synthesize FGF23. Serum concentrations of calcium, phosphate (Pi) and FGF23 were measured in control nu/nu mice, bone-metastasized mice, and mice with mammary gland injected with MDA-MB-231 cells mimicking primary mammary tumors. Results and conclusions MDA-MB-231 cells revealed intense FGF23 reactivity in metastasized lesions, whereas MDA-MB-231 cells cultured in vitro or when injected into the mammary glands (without bone metastasis) showed weak FGF23 immunoreactivity. Although the bone-metastasized MDA-MB-231 cells abundantly synthesized FGF23, osteocytes adjacent to the FGF23-immunopositive tumors, unlike intact osteocytes, showed no FGF23. Despite significantly elevated serum FGF23 levels in bone-metastasized mice, there was no significant decrease in the serum Pi concentration when compared with the intact mice and mice with a mass of MDA-MB-231 cells in mammary glands. The metastasized femora showed increased expression and FGFR1 immunoreactivity in fibroblastic stromal cells, whereas femora of control mice showed no obvious FGFR1 immunoreactivity. Taken together, it seems likely that MDA-MB-231 cells synthesize FGF23 when metastasized to a bone, and thus affect FGFR1-positive stromal cells in the metastasized tumor nest in a paracrine manner
ASIC FFT processor for MB-OFDM UWB system
No full textThe physical layer (PHY) standard of Multi-band Orthogonal Frequency Division Multiplexing (MB-OFDM) Ultra Wideband (UWB) system was defined by ECMA International. In this standard, the data sampling rate from the analog-to-digital converter to the physical layer is up to 528 Msample/s. Therefore, it is a challenge to realize the physical layer of the UWB system-especially the components with high computational complexity in Very Large Scale Integration (VLSI) implementation. Fast Fourier Transform (FFT) block is one of these components. FFT plays an important role in Multi-band OFDM UWB system, which is the demodulation block of OFDM signals. The purpose of this project is to design an Application Specific Integrated Circuit (ASIC) FFT solution for this system. The specification is defined from the system analysis and literature research. All the design choices and considerations are concluded and explained.Based on the algorithm and architecture analysis, a novel Radix22Parallel processor is proposed, which is a small-area and low-power-consumption solution for MB-OFDM UWB system. Both Field Programmable Gate Array (FPGA) and ASIC targeted synthesis results of this architecture are presented.Electrical Engineering, Mathematics and Computer Scienc
Characterization of the tertiary structure of the de novo designed protein MB-1
No full textMilk Bundle-1 (MB-1) is a de novo designed protein with 100 amino acids, having a molecular weight of 11.4 kilodaltons. MB-1 is enriched with 57% of selected essential amino acids (methionine, threonine, lysine and leucine), which are known to be limiting in dairy cattle. Recently, on the basis of a digestibility study, MB-1 was predicted to be unstable in rumen conditions.Characterization of the protein's structure was achieved using fluorescence spectroscopy (steady state measurements). MB-1 contains one tyrosine at position 62, expected to be in position "d" of helix III, in the hydrophobic core. Data obtained using fluorescence quenching indicates that the tyrosine is protected from the solvent in the putative hydrophobic core, as per design.Once it was established that MB-1 was not misfolded, further experiments were done to assess the fluidity of its hydrophobic core. For this, the amphiphillic dye ANSA was used. Results obtained for MB-1 compare favourably to those of many natural proteins, suggesting that MB-1 has achieved some degree of nativeness. Interestingly, MB-1 was found to exclude ANSA from its hydrophobic core more efficiently than all other de novo designed proteins reported to date.Finally, an analysis of folding thermodynamics of MB-1 was attempted. It was found that the fluorescence intensity of tyrosine was not sensitive to unfolding, making thermodynamic data impossible to obtain.Analysis of the data on MB-1 as compared to other natural proteins indicates that MB-1 is folded and compact. The lack of resistance to proteases must be caused by other factors other than the lack of compactness or misfolding. (Abstract shortened by UMI.).Source: Masters Abstracts International, Volume: 36-06, page: 1623.Adviser: Marc Beauregard
Stochastic Lie bracket (derivation, derivation) in MB-algebras
No full textBy a stochastic controller, we make stable the pseudo stochastic Lie bracket (derivation, derivation) in complex MB-algebras. Next, we get an approximation by a stochastic Lie bracket (derivation, derivation) and calculate the maximum error of the estimate. © 2020, The Author(s)
Weerkat: An extensible semantic Wiki
No full textWikis are Web applications that blur the boundaries between readers and authors, allowing non-technical people to author hypertexts through a web interface. A Semantic Wiki is a Wiki that attempts the same thing with the Semantic Web, allowing non-technical users to create semantic resources and/or ontologies. In this paper we characterise the different ways in which a Wiki might support the Semantic Web and present Weerkat, a modular and extensible Wiki that has ontological hypertext support. Key to this has been the design and development of a highly flexible Wiki architecture which allows easy modification and augmentation of functionality
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