75 research outputs found
Nain's Hierarchy of Needs: An Alternative to Maslow's & ERG's Hierarchy of Needs
No full textThis article gives reasons as to why Maslow's & ERG Theory of Needs is inaccurate. It also gives reasons why the same is inaccurate in an organizational perspective. The author also gives a alternative model of needs, namely the Nain Model, which is particularly applicable in an organizational perspective. This article has been written for those interested in Organizational Behaviour
TALENs—an indispensable tool in the era of CRISPR: a mini review
No full textAbstract Background Genome of an organism has always fascinated life scientists. With the discovery of restriction endonucleases, scientists were able to make targeted manipulations (knockouts) in any gene sequence of any organism, by the technique popularly known as genome engineering. Though there is a range of genome editing tools, but this era of genome editing is dominated by the CRISPR/Cas9 tool due to its ease of design and handling. But, when it comes to clinical applications, CRISPR is not usually preferred. In this review, we will elaborate on the structural and functional role of designer nucleases with emphasis on TALENs and CRISPR/Cas9 genome editing system. We will also present the unique features of TALENs and limitations of CRISPRs which makes TALENs a better genome editing tool than CRISPRs. Main body Genome editing is a robust technology used to make target specific DNA modifications in the genome of any organism. With the discovery of robust programmable endonucleases-based designer gene manipulating tools such as meganucleases (MN), zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats associated protein (CRISPR/Cas9), the research in this field has experienced a tremendous acceleration giving rise to a modern era of genome editing with better precision and specificity. Though, CRISPR-Cas9 platform has successfully gained more attention in the scientific world, TALENs and ZFNs are unique in their own ways. Apart from high-specificity, TALENs are proven to target the mitochondrial DNA (mito-TALEN), where gRNA of CRISPR is difficult to import. This review talks about genome editing goals fulfilled by TALENs and drawbacks of CRISPRs. Conclusions This review provides significant insights into the pros and cons of the two most popular genome editing tools TALENs and CRISPRs. This mini review suggests that, TALENs provides novel opportunities in the field of therapeutics being highly specific and sensitive toward DNA modifications. In this article, we will briefly explore the special features of TALENs that makes this tool indispensable in the field of synthetic biology. This mini review provides great perspective in providing true guidance to the researchers working in the field of trait improvement via genome editing
KasI vs. SspDI: Comparative analysis of enzyme activity for restriction digestion
No full text945-948Type II restriction enzymes are routinely used by molecular biologists in designing and implementation of cloning experiments without referring to the literature on enzymes in use, and at times, face some unforeseeable problems. In our laboratory too, we encountered one such problem while working with KasI restriction enzyme which recognizes GGCGCC sequence, and we further analyzed the issue. Our observations corroborate the fact that KasI acts as monomer and cleaves double stranded DNA through nicking mechanism. It introduces breaks in two strands of DNA after substantial time gap which can be owed to two independent nickase activities in the opposite strands. Moreover, this time gap between two nickase activities results in formation of different topological forms of DNA. Since molecular biologists working with common restriction enzymes are not familiar with such nickase activity, they may misinterpret their restriction digestion results. However, no such problem was observed with the use of SspDI restriction enzyme which also recognizes the same sequence (GGCGCC) and produces the identical overhangs as by KasI. Hence, SspDI suits better for routine cloning and genetic modification purposes over KasI while using GGCGCC as cloning site
La resurrección del hijo de la Viuda de Nain Aproximación narrativa al relato de Lucas 7,11-17
No full textThe miracle of "Raising the Widow\u27s Son at Nain", is a gripping tale where actors appear and disappear from the stage play as if it were a “real work”. Jesus with the widow, are key players in this beautiful \u27work\u27. The boy brought back to life is the maximum expression of the love of God that "reveals himself to his people". All those present praising the God of the promises, is the summit and the meaning of the presence of the true gift of the creator: “Life”. A narrative look at Lucas narration makes us probe the mysterious inner world of the author of the Gospel and its relationship with the God of Jesus.El milagro de La resurrección del hijo de la viuda de Nain, es un relato apasionante, donde los actores aparecen y desaparecen de la escena como si se tratase de una verdadera obra. Jesús junto a la viuda, son protagonistas en esta hermosa obra. El muchacho vuelto a la vida, es la expresión máxima del amor de Dios que visita a su pueblo. Todos los presentes alabando y bendiciendo al Dios de las promesas, es el culmen y el sentido, de la presencia del verdadero don del creador: La Vida. Una mirada narrativa al relato de Lucas nos hace sondear el misterioso mundo interior del autor del evangelio y su relación con el Dios de Jesús
Inuit plant use in the eastern Subarctic: comparative ethnobotany in Kangiqsualujjuaq, Nunavik and Nain, Nunatsiavut
No full textPlants are important in traditional Inuit life. They are used for food, tea, medicine, etc. Based on semi-structured interviews with 35 informants, we documented and compared plant names and uses in Nain, Nunatsiavut and Kangiqsualujjuaq, Nunavik. Plant names and uses were expected to be similar between communities owing to common boreal-subarctic environments and cultural ties. Both communities reported the same number of taxa, with equivalent proportions of vascular/non-vascular, growth forms, use categories, and medicinal uses. Forty-three species were used in each community, for a total of 78 species from 39 families. Despite a high overlap in species distributions, only 35% of non-vascular and 56% of vascular species were used in both communities. Correspondence was higher at the family level (64% of non-vascular and 75% of vascular families shared). The Ericaceae family was the most-used, followed by Rosaceae. Thirteen of 30 medicinal species were shared between communities. There was a low correspondence regarding the conditions for which the medicinal species were used. Edible taxa were shared the most (52%). Plant uses unique to either Nain or Kangiqsualujjuaq may reveal separate bodies of traditional knowledge, or may reflect an overall loss of ethnobotanical knowledge in the Subarctic due to recent lifestyle changes.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Optimization of fermentation condition for co-production of ethanol and 2,3-butanediol (2,3-BD) from hemicellolosic hydrolysates by <i>Klebsiella oxytoca</i> XF7
No full textCPP-ZFN: A potential DNA-targeting anti-malarial drug
No full textAbstract Background Multidrug-resistant Plasmodium is of major concern today. Effective vaccines or successful applications of RNAi-based strategies for the treatment of malaria are currently unavailable. An unexplored area in the field of malaria research is the development of DNA-targeting drugs that can specifically interact with parasitic DNA and introduce deleterious changes, leading to loss of vital genome function and parasite death. Presentation of the hypothesis Advances in the development of zinc finger nuclease (ZFN) with engineered DNA recognition domains allow us to design and develop nuclease of high target sequence specificity with a mega recognition site that typically occurs only once in the genome. Moreover, cell-penetrating peptides (CPP) can cross the cell plasma membrane and deliver conjugated protein, nucleic acid, or any other cargo to the cytoplasm, nucleus, or mitochondria. This article proposes that a drug from the combination of the CPP and ZFN systems can effectively enter the intracellular parasite, introduce deleterious changes in its genome, and eliminate the parasite from the infected cells. Testing the hypothesis Availability of a DNA-binding motif for more than 45 triplets and its modular nature, with freedom to change number of fingers in a ZFN, makes development of customized ZFN against diverse target DNA sequence of any gene feasible. Since the Plasmodium genome is highly AT rich, there is considerable sequence site diversity even for the structurally and functionally conserved enzymes between Plasmodium and humans. CPP can be used to deliver ZFN to the intracellular nucleus of the parasite. Signal-peptide-based heterologous protein translocation to Plasmodium-infected RBCs (iRBCs) and different Plasmodium organelles have been achieved. With successful fusion of CPP with mitochondrial- and nuclear-targeting peptides, fusion of CPP with 1 more Plasmodium cell membrane translocation peptide seems achievable. Implications of the hypothesis Targeting of the Plasmodium genome using ZFN has great potential for the development of anti-malarial drugs. It allows the development of a single drug against all malarial infections, including multidrug-resistant strains. Availability of multiple ZFN target sites in a single gene will provide alternative drug target sites to combat the development of resistance in the future.</p
MYOD and HAND transcription factors have conserved recognition sites in mTOR promoter: insights from in silico analysis
No full textMoravian sermon, 1938
No full textThis item is a Moravian sermon used for Advent service in Nain, Labrador, 1938. The author is unknown.Title based on the contents of the record
Plate Heat Exchanger Optimization Using Different Approximation Assisted Multiobjective Optimization Techniques
No full textThis paper presents a comparison between different multiobjective optimization approaches that can be used to optimize the design of thermal equipment. Plate heat exchanger is taken as case study to apply different optimization techniques. The thermal-hydrodynamic characteristics of single phase turbulent flow in chevron-type plate heat exchangers with sinusoidal-shaped corrugations have been used in this paper. The computational domain contains a corrugation channel and the simulations adopted the shear-stress transport (SST) κ-ω model as the turbulence model. Two different approximation assisted optimization approaches are tested. Offline approximation assisted optimization, and online approximation assisted optimization are compared to optimize plate heat exchanger design. For both approximation techniques (offline and online), design optimization is performed using multiobjective genetic algorithm based on meta-models that are built to represent the entire design space. In offline approximation, globally accurate meta-models are built which requires adding more samples. However in online approximation assisted optimization, samples are added just to improve the metamodels performance in the expected optimum region. Approximated optimum designs are validated using computationally expensive actual CFD simulations. Finally, a comparison between offline and online approximation assisted optimization is presented with guidelines to apply both approaches in the area of heat exchanger design optimization. The methods presented in this paper are generic and can be applied to optimize different types of heat exchangers, electronic cooling devices and other thermal system components
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