5,514 research outputs found
Puesta a punto y comparación del desempeño de un reactivo nacional contra métodos de rutina en el laboratorio bioquímico para la detección del Virus Sincicial Respiratorio (VSR) por RT-qPCR
No full textLa infección respiratoria aguda (IRA) producida por VSR es una de las principales causas de hospitalizaciones de lactantes y niños y de una elevada morbilidad y mortalidad entre ancianos y adultos con condiciones debilitantes subyacentes. El diagnóstico preciso y rápido disminuye el uso innecesario de antibióticos y pruebas adicionales de laboratorio, el tiempo de hospitalización y la transmisión intrahospitalaria. La PCR en tiempo real es una metodología adecuada para su utilización en el laboratorio bioquímico por su velocidad, sensibilidad y la posibilidad de multiplexado, es decir la detección de cuatro o cinco patógenos simultáneamente (dependiendo de la cantidad de canales del instrumento usado).Fil: Viceconte, Sebastian. Universidad Nacional Arturo Jauretche. Instituto de Ciencias de la Salud; Argentina.Fil: Castello, Alejandro. Universidad Nacional Arturo Jauretche. Instituto de Ciencias de la Salud; Argentina.Fil: Diaz, Rosa Viviana. Universidad Nacional Arturo Jauretche. Instituto de Ciencias de la Salud; Argentina
Novel dietary supplement association reduces symptoms in endometriosis patients
No full textEndometriosis is characterized by disabling symptoms that afflict young women with severe physical discomfort, difficulty in relationship life, and infertility; however, the currently available therapeutic strategies are unsatisfactory. Goal of this research was to identify a new combination of natural active ingredients that, administered as dietary supplements, could have the effect of reducing inflammatory response in endometriosis patients, decreasing the symptoms the disease produces and its harmful effects on affected organs. A cohort of endometriosis patient was treated for three months with a composition including quercitin, curcumin, parthenium, nicotinamide, 5-methyltetrahydrofolate and omega 3/6. Using a VAS scale, we demonstrated a significant reduction of the symptoms in endometriosis patients treated with the dietary composition respect to the controls. Moreover, we demonstrated also a significant reduction in the serum levels of PGE2 and CA-125. Further study are required to compare the effect of this combination of molecules with standard therapies and to evaluate if the use of these dietary supplements in combination with standard therapies may lead to the improvement of the regular medical treatment for endometriosis. This article is protected by copyright. All rights reserved.Endometriosis is characterized by disabling symptoms that afflict young women with severe physical discomfort, difficulty in relationship life, and infertility; however, the currently available therapeutic strategies are unsatisfactory. Goal of this research was to identify a new combination of natural active ingredients that, administered as dietary supplements, could have the effect of reducing inflammatory response in endometriosis patients, decreasing the symptoms the disease produces and its harmful effects on affected organs. A cohort of endometriosis patient was treated for 3 months with a composition including quercitin, curcumin, parthenium, nicotinamide, 5-methyltetrahydrofolate, and omega 3/6. Using a VAS scale, we demonstrated a significant reduction of the symptoms in endometriosis patients treated with the dietary composition respect to the controls. Moreover, we demonstrated also a significant reduction in the serum levels of PGE2 and CA-125. Further study are required to compare the effect of this combination of molecules with standard therapies and to evaluate if the use of these dietary supplements in combination with standard therapies may lead to the improvement of the regular medical treatment for endometriosis
New Insights in Pathogenesis of Endometriosis
No full textEndometriosis is a gynecological disease characterized by the growth of endometrial glands and stroma outside the uterine cavity. The incidence of the disease is very high, there are currently no reliable early diagnostic tests, the therapies are only symptomatic and, consequently, the social impact of endometriosis is very important, also considering the related fertility problems. Despite this, the pathogenesis of endometriosis is still not fully defined. Retrograde menstruation and coelomic metaplasia are currently the most recognized pathogenetic hypotheses. Recent experimental evidences generated by our research group and by others have indicated an alteration of the fine-tuning of the female genital system developmental program during a critical window of time in the fetal life as the pathogenetic event prompting to the development of endometriosis later in life. Goal of this article is to present a revision of the recent literature about the different pathogenetic mechanisms proposed for endometriosis with particular emphasis on the embryologic theory. The possible clinical and pathological implications of these findings will be discussed
Differential Expression in Endometriosis Tissue versus Endometrium of the Uterine Adenogenesis Factors PRL-R, GH, IGF1, and IGF2
No full textEndometriosis is characterized by the presence of endometrial glandular and stromal structures outside the uterine cavity. It is an inflammatory estrogen dependent disease characterized by gene polymorphisms. This is a very frequent pathology and represents one of the most important causes of infertility, as well as having an important level of morbidity in patients. Recently, an alteration of the processes of organogenesis of the uterus has been proposed as a pathogenetic mechanism of endometriosis. In this article we have compared the expression in deep endometriotic lesions and in normal endometrial tissue of some of the molecular factors known to be involved in the embryonic development of the uterine glands. In detail, we found by immunohistochemistry a significant higher expression both for epithelium and stroma in the controls respect to the endometriosis samples for insulin growth factor 1 (IGF1) and IGF2, whereas for the prolactin receptor (PRL-R), this result was detected only for the epithelium. On the other hand, we found for growth hormone (GH) a significant higher expression in the epithelium of endometriosis samples respect to the controls. The correlation data generated can give indications on some of the molecular mechanisms responsible for the adenogenesis and survival of endometriosis structures outside of the uterus
I want to learn more! Integrating technology acceptance and task–technology fit models for predicting behavioural and future learning intentions
No full textPrototype Salivary Assay for Quantification of Two Biomarkers for In Vitro Diagnosis of Endometriosis
No full text: Endometriosis, a very common disease in women, is characterized by endometrial structures outside the uterine cavity. The lack of a reliable noninvasive diagnostic test and the often nonspecific symptoms of this pathology are responsible for the delay in definitive diagnosis of this disease. Recently, through a proteomics approach, our research group has identified two potential diagnostic markers for endometriosis in serum (Zn-alpha2-glycoprotein and complement C3 precursor). In this article, we describe the experimental conditions of a simple ELISA for rapid quantification of these two biomarkers in the saliva of patients with endometriosis. Finally, preliminary experiments on a small cohort of patients and controls have confirmed the potential diagnostic value of this assay
Pathogenesis of Endometriosis: Focus on Adenogenesis-related Factors
No full text: Endometriosis can be defined as the presence of the endometrium outside the uterine cavity. It affects approximately 10% of women of reproductive age and causes infertility, chronic pain, and deterioration of the quality of life. Since the identification of the disease, various pathogenetic mechanisms have been proposed, such as retrograde menstruation, coelomic metaplasia, hormonal imbalance, stem cell involvement, and alterations in epigenetic regulation. However, the underlying pathogenesis of endometriosis remains inadequately understood. Elucidation of the precise mechanism of the development and progression of endometriosis is crucial for effective treatment. This review presents the major pathogenetic theories of endometriosis based on current research studies with a major focus on the potential role of uterine factors
tau; and HGF in Endometriosis Tissue Respect to Eutopic Endometrium: An Immunohistochemical Study
No full text: Endometriosis is a pathological condition defined by the occurrence of endometrial glandular and stromal structures in anatomical compartments different from the uterine cavity. Endometriosis is a genetic polymorphism, estrogen-dependent inflammatory disease. This very common pathological entity causes a high level of morbidity in patients; it is also considered one of the most important causes of infertility. We and others have proposed as a pathogenetic mechanism of endometriosis a modification in the fine tuning of the processes of organogenesis of the uterus. We have correlated the immunohistochemical expression in deep endometriotic lesions and in normal endometrial tissue of several molecular factors that are implicated in the embryonic development of the uterine glands. We noticed a significant higher expression both for epithelium and stroma in the controls respect to the endometriosis samples for FGF7, FGF-10 and HGF. Interestingly, regarding FGF-23 and IFN-τ, we observed a significant higher expression in the ectopic endometrial stroma compared to the eutopic endometrium, while thepithetlium expression did not display a significant differential expression in endometriosis tissues respect to normal endometrium. The data generated support the fact that endometriosis tissues, both the epithelial and stromal component, have a different phenotype respect to the eutopic endometrium and sustain the hypothesis that alterations in the molecular mechanisms in control for adenogenesis and survival of endometrial structures are linked to the genesis and survival of endometriosis lesions outside of the uterus
The life and Story of Rosa Maria:Brazil's First Black Author
No full textThirty years after the release of Rosa Maria Egipcíaca's biography by Brazilian historian LuizMott in 1993, one of Rio de Janeiro's strongest carnival samba schools, Unidos do Viradouro, finally brought her name into Brazilian mainstream culture. Rosa Maria is Brazil's first black author, having written a book and letters of prophecy. Her story is global in both space and time. In her journey, Rosa traversed three different continents - Africa, South America and Europe - interconnected by the colonial ties of transatlantic slavery and Catholicism, and though she lived in the eighteenth century, her 'afterlife' extends until today, as will be described through the context of Brazil's carnival
Sub Rosa
No full textIn this stunning debut novel, Amber Dawn subverts and transgresses the classic hero's quest adventure to create a dark post-feminist vision not for the faint of heart. Sub Rosa's reluctant heroine is known as "Little," a teenaged runaway unable to remember her real name; in her struggle to get by in the world, she stumbles upon an underground society of ghosts and magicians, missing girls and would-be johns: a place called Sub Rosa. Not long after she is initiated into this family of magical prostitutes, Little is called upon to lead Sub Rosa through a maze of feral darkness, both real and imagined--a calling burdened with grotesque enemies, strange allies, and memories from a foggy past.
Written with a kind of gasping urgency, Sub Rosa is a beautiful and gutsy allegory of our times, a fairy-tale-like fantasia imbued with a grave, unapologetic realness. --From publisher description.Women's literatureFictionLGBTQ+Lesbian literatur
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