86,648 research outputs found
Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines
No full textThe regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl alpha-(2→6)-galactosides stimulated cell proliferation
Fabrication strategies towards hydrogels for biomedical application: chemical and mechanical insights
No full textThis review aims at giving selected chemical and mechanical insights on design criteria that should be taken into account in hydrogel production for biomedical applications. Particular emphasis will be given to the chemical aspects involved in hydrogel design: macromer chemical composition, cross-linking strategies and chemistry towards “conventional” and smart/stimuliresponsive hydrogels. Mechanical properties of hydrogels in view of regenerative medicine applications will also be considered
Dall'ibristofilia al narcisismo: il fascino del male = From hybristophilia to narcissism: the charm of evil
No full textTwo cases of parenticide in Italy, which had a wide echo and which are briefly reported, provide the Authors with the opportunity to describe the phenomenon of hybristophilia, that is the attraction for a criminal as such, and to report what is proposed by the scientific literature on the subject.
Both cases were followed by the creation of "fan groups".
In the most recent case, the attraction for the parenticide led to the creation of a Facebook group: the Authors analyze 575 messages from this group, elaborate a typology, and draw conclusions about the motivations, the potential dangerousness of the group members, the narcissism underlying the type of communication analyzed
Estimation of the binding force of the collagen molecule-decorin core protein complex in collagen fibril
No full textDecorin belongs to the small leucine proteoglycans family and is considered to play an important role in extracellular matrix organization. Experimental studies suggest that decorin is required for the assembly of collagen fibrils, as well as for the development of proper tissue mechanical properties. In tendons, decorins tie adjoining collagen fibrils together and probably guarantee the mechanical coupling of fibrils. The decorin molecule consists of one core protein and one glycosaminoglycan chain covalently linked to a serine residue of the core protein. Several studies have indicated that each core protein binds to the surface of collagen fibrils every 67 nm, by interacting non-covalently to one collagen molecule of the fibril surface, while the decorin glycosaminoglycans extend from the core protein to connect to another decorin core protein laying on adjacent fibril surface. The present paper investigates the complex composed of one decorin core protein and one collagen molecule in order to obtain their binding force. For this purpose, molecular models of collagen molecules type I and decorin core protein were developed and their interaction energies were evaluated by means of the molecular mechanics approach. Results show that the complex is characterized by a maximum binding force of about 12.4 x 10(3) nN and a binding stiffness of 8.33 x 10(-8) N/nm; the attained binding force is greater than the glycosaminoglycan chain's ultimate strength, thus indicating that overloads are likely to damage the collagen fibre's mechanical integrity by disrupting the glycosaminoglycan chains rather than by causing decorin core protein detachment from the collagen fibril
Design of a Collagen/Silk Mechano-Compatible Composite Scaffold for the Vascular Tissue Engineering: Focus on Compliance
No full textResponse to letter to the editor: On the calculation of the binding force between decorin and collagen
No full textCharacterization of mechanical properties of ECM nanostructures of tendons and ligaments
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Biological performances of collagen-based scaffolds for vascular tissue engineering
No full textCollagen is widely used for biomedical applications and it could represent a valid alternative scaffold material for vascular
tissue engineering.In this work, reconstituted collagen films were prepared from neutralized acid-soluble solutions for subsequent
haemocompatibility and cell viability performance assays.First, haemoglobin-free, thrombelastography and platelet adhesion tests
were performed in order to investigate the blood contact performance.Secondly, specimens were seeded with endothelial cells and
smooth muscle cells, and cell viability tests were carried out by MTT and SEM.Results show that neutralized acid-soluble type I
collagen films do not enhance blood coagulation, do not alter normal viscoelastic properties of blood and slightly activate platelet adhesion and aggregation.Cell culture shows that the samples are adequate substrates to support the adhesion and proliferation of endothelial and smooth muscle cells
Die distale spleno-renale termino-laterale Anastomose nach Warren
No full textThis is a report on 28 Warren-Shunts, 17 of these patients had undergone clinical, hematological and angiographic controls 2-46 (mean 10) months after the operation. The critical evaluation of the results shows that the Warren derivation, which is selective at the moment of operation, develops in the following time some hepatofugal circulation. This is a more radical effect than the intended drainage of the gastro-esophageal venous bed alone. © 1979 Springer-Verlag
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