1,721,056 research outputs found

    Report of Meeting. Third International Symposium on Advances in Marine Mussel Research 2019 (AMMR 2019), August, 26-28, 2019, Palazzo Grassi, Chioggia, Italy.

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    REPORT OF MEETING. Third International Symposium AMMR 2019. Advances in Marine Mussel Research, August, 26-28, 2019, Palazzo Grassi, Chioggia, Italy. Organizers: L Ballarin, U Rosani and P Venier (Chair). International Committee: N Bierne, Á Pérez Diz, A Figueras, M Gerdol, B Novoa, A Pallavicini, U Rosani and P Venier. Total 58 delegates, from 14 countries and 30 different cities of the world, gathered in Chioggia (VE) in the occasion of AMMR 2019. The event was organized in partnership by the University of Padova, Department of Biology and School of Biosciences, under the patronage of Veneto Region, the town of Chioggia and the Horizon 2020 project VIVALDI (see other sponsors at https://www.biologia.unipd.it/ammr -2019). News and views have been exchanged on i) genetic mussel traits, including a significant molecular evidence of gene presence/absence variation, ii) population markers and evolution of the Mytilus species complex, iii) functional mussel responses in relation to potential pathogens and other factors. Innovative experimental strategies applied to marine mussels as well as mussel contributions in goods and services confirm these fascinating marine invertebrates as a model of study and strategic resource for the future. The next AMMR symposium is expected in 2021 in Poland

    Inquinanti genotossici nell'acqua

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    L’inquinamento delle acque è un fenomeno globale entro il quale gli interrogativi posti dalla presenza di agenti mutageni, cancerogeni e teratogeni non possono essere considerati disgiuntamente da quelli relativi ad altri agenti tossici. I contaminanti genotossici presenti nell’ambiente acquatico possono essere individuati saggiando in vitro campioni ambientali e rilevando il danno genetico indotto dopo l’esposizione in organismi sentinella. Evidenze di questo tipo sono un segnale d’allarme per le potenziali conseguenze a lungo termine, definite dall’instaurarsi di cambiamenti nelle popolazioni, nelle specie e nelle comunità degli organismi esposti. L’applicazione di biomarcatori di esposizione e di effetto in associazione alle analisi chimiche può fornire le informazioni utili a individuare le fonti inquinanti e a stabilire adeguate misure di protezione dei corpi idrici

    An evolutionary perspective of dopachrome tautomerase enzymes in metazoans

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    Melanin plays a pivotal role in the cellular processes of several metazoans. The final step of the enzymically-regulated melanin biogenesis is the conversion of dopachrome into dihydroxyindoles, a reaction catalyzed by a class of enzymes called dopachrome tautomerases. We traced dopachrome tautomerase (DCT) and dopachrome converting enzyme (DCE) genes throughout metazoans and we could show that only one class is present in most of the phyla. While DCTs are typically found in deuterostomes, DCEs are present in several protostome phyla, including arthropods and mollusks. The respective DCEs belong to the yellow gene family, previously reported to be taxonomically restricted to insects, bacteria and fungi. Mining genomic and transcriptomic data of metazoans, we updated the distribution of DCE/yellow genes, demonstrating their presence and active expression in most of the lophotrochozoan phyla as well as in copepods (Crustacea). We have traced one intronless DCE/yellow gene through most of the analyzed lophotrochozoan genomes and we could show that it was subjected to genomic diversification in some species, while it is conserved in other species. DCE/yellow was expressed in most phyla, although it showed tissue specific expression patterns. In the parasitic copepod Mytilicola intestinalis DCE/yellow even belonged to the 100 most expressed genes. Both tissue specificity and high expression suggests that diverse functions of this gene family also evolved in other phyla apart from insects

    Diversity of Coding Sequences and Gene Structures of the Antifungal Peptide Mytimycin (MytM) from the Mediterranean Mussel, Mytilus galloprovincialis

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    Knowledge on antifungal biomolecules is limited compared to antibacterial peptides. A strictly antifungal peptide from the blue mussel, Mytilus edulis named mytimycin (MytM) was reported in 1996 as partial NH2 33 amino acid sequence. Using back-translations of the previous sequence, MytM-related nucleotide sequences were identified from a normalized Mytilus galloprovincialis expressed sequence tag library. Primers designed from a consensus sequence have been used to obtain a fragment of 560 nucleotides, including the complete coding sequence of 456 nucleotides. Precursor is constituted by a signal peptide of 23 amino acids, followed by MytM of 54 amino acids (6.2-6.3 kDa, 12 cysteines) and C-terminal extension of 75 amino acids. Only two major amino acid precursor sequences emerged, one shared by M. galloprovincialis from Venice and Vigo, the other belonging to M. galloprovincialis from Palavas, with nine amino acid differences between the two MytM. Predicted disulfide bonds suggested the presence of two constrained domains joined by amino acidic NIFG track. Intriguing was the presence of conserved canonical EF hand-motif located in the C-terminus extension of the precursor. The MytM gene was found interrupted by two introns. Intron 2 existed in two forms, a long (1,112 nucleotides) and a short (716 nucleotides) one resulting from the removal of the central part of the long one. Both the short (GenBank FJ804479) and the long (GenBank FJ804478) genes are simultaneously present in the mussel genome

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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