4,734 research outputs found
Analysis of sesquiterpene pyridine alkaloids using ESI-MS/MS: The product ion at m/z 310 formed by an ion-dipole intermediate
No full textSesquiterpene pyridine alkaloids are a large group of highly oxygenated sesquiterpenoids, attracting attention in the fields of medicine because of their significant biological activities. Three representative sesquiterpene pyridine alkaloids were analyzed by electrospray ionization collision-induced dissociation-tandem mass spectrometry (ESI-CID-MS/MS/MS). In the high mass range, the product ions were formed by the loss of side chains or H2O. In the low mass range, the high-abundance product ions at m/z 206 and 178 were the characteristic ions of the pyridine moiety. Interestingly, the characteristic product ion at m/z 310 was formed through an ion-dipole (ion-neutral) complex. Addition of oxo(phenyl)methylium formed by the loss of the side chain linked to C1 and pyridine moiety produces m/z 310. The product ions at m/z 188 and 105 from m/z 310 support the proposed processes. The product ions were supported by D-labeling experiment. It was found that the formation of the product ion at m/z 310 was related to the position of substituted groups. (C) 2014 Elsevier B.V. All rights reserved
The Poems of MS Junius 11 Basic Readings
No full textTaken from the same manuscript as Cynewulf, the Junius 11 poems-Genesis, Exodus, Daniel, and Christ and Satan-comprise a series of redacted Old English works that have been traditionally presented as the work of Bede's Caedmon. Medieval scholars have concluded that the four poems were composed by more than one author and later edited by Junius in 1655. All of the poems are notable for their Christian content. Apart from its focus on the Junius 11 manuscript, this collection of essays is also important as a study of how to read, edit, and define any medieval literary text.Front Cover -- The Poems of MS Junius 11 -- Copyright Page -- Contents -- Preface of the General Editors -- Introduction: R. M. Liuzza -- Acknowledgments -- List of Abbreviations -- Confronting Germania Latina: Changing Responses to Old English Biblical Verse: Joyce Hill -- The Old English Epic of Redemption: The Theological Unity of MS Junius 11: J. R. Hall -- The Old English Epic of Redemption": Twenty-Five-Year Retrospective: J. R. Hall -- Some Uses of Paronomasia in Old English Scriptural Verse: Roberta Frank -- Tempter as Rhetoric Teacher: The Fall of Language in the Old English Genesis B: Eric Jager -- Conspicuous Heroism: Abraham, Prudentius, and the Old English Verse Genesis: Andrew Orchard -- Christian Tradition in the Old English Exodus: James W. Earl -- The Patriarchal Digression in the Old English Exodus, Lines 362-446: Stanley R. Hauer -- The Lion Standard in Exodus: Jewish Legend, Germanic Tradition, and Christian Typology: Charles D. Wright -- The Structure of the Old English Daniel: Robert T. Farrell -- Style and Theme in the Old English Daniel: Earl R. Anderson -- Nebuchadnezzar's Dreams in the Old English Daniel: Antonina Harbus -- The Power of Knowledge and the Location of the Reader in Christ and Satan: Ruth Wehlau -- The Wisdom Poem at the End of MS Junius 11: Janet Schrunk Ericksen -- IndexTaken from the same manuscript as Cynewulf, the Junius 11 poems-Genesis, Exodus, Daniel, and Christ and Satan-comprise a series of redacted Old English works that have been traditionally presented as the work of Bede's Caedmon. Medieval scholars have concluded that the four poems were composed by more than one author and later edited by Junius in 1655. All of the poems are notable for their Christian content. Apart from its focus on the Junius 11 manuscript, this collection of essays is also important as a study of how to read, edit, and define any medieval literary text.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Simultaneous determination of diphenylarsinic and phenylarsinic acids in amended soils by optimized solvent extraction coupled to HPLC-MS/MS
No full textA solvent extraction method coupled to high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) was developed and validated for the simultaneous determination of soil diphenylarsinic acid (DPAA) and phenylarsinic acid (PAA). Several extraction solvents were evaluated with respect to the degree of soil extract-induced matrix effects and the recovery values for DPAA and PAA in the four studied soil matrices. Validation data showed that solvent extraction had a negligible impact on the accurate quantification of DPAA. In contrast, only with disodium hydrogen phosphate extraction did the method give both limited matrix effects (102-107%) with a coefficient of variation 74.03%) were obtained for both analytes. The results show that the proposed solvent extraction method coupled to HPLC-MS/MS analysis can provide accurate and reliable determinations of DPAA and PAA in soil samples. (C) 2015 Elsevier B.V. All rights reserved
Analysis of iridoid glucosides from Paederia scandens using HPLC-ESI-MS/MS
No full textIridoid glycosides are an important class of natural products and have many biological activities. Iridoid glucosides in an extract of the plant species Paederia scandens were investigated using reversed-phase high performance liquid chromatography and electrospray quadrupole time-of-flight-type tandem mass spectrometry. The elemental composition of most of the compounds was determined by accurate mass and relative isotopic abundance (RIA) measurements. In positive ion mode, the fragmentation of [M+NH4](+) precursor ions was carried out using low energy collision-induced electrospray ionization tandem spectrometry. The neutral losses of NH3, H2O, Glc, and the side chain of the iridoid moiety were the main fragmentation patterns observed. For simple iridoid glycosides, the main differences were related to the side chains. Fragmentation of the [M-H](-) precursor ions was achieved for the compounds possibly having phenolic acid group. The connection order of the iridoid, sugar, and phenolic acid moieties, and the linkage of the 6-OH group of the sugar to the phenolic acid were unambiguously confirmed using a combination of MS/MS spectra in both positive and negative ion modes, and our previous work. For some trace dimeric iridoid glucosides, the connection order between the asperuloside and paederoside moieties was determined by the characteristic product ions; this was supported by D-labeling experiments. A total of 24 iridoid glucosides, including 14 new species, were identified or tentatively characterized based on exact mass, RIA values, tandem mass spectra, and D-labeling experiments. (c) 2013 Elsevier B.V. All rights reserved
System design department for acquisition process at M&A department and its implementation in MS Sharepoint 2013
No full textThis thesis analyse the implementation of the MS SharePoint solution to the acquisition process of the selected M&A department of multinational enterprise. Analysis of the possible options to use the MS SharePoint 2013 platform for solution of the specific acquisition processes, descriptions of the recommendations for setting-up of the processes via MS SharePoint 2013 and implementation of the MS SharePoint 2013 solution in specific M&A department are the main goals of this thesis. The abovementioned goals are achieved through research in the relevant literature, research papers, based on the lectures provided by company Gopas and consultation with experts from Microsoft company as well as the practical experience of the author of this thesis. The main benefit of this thesis is the comprehensive review of the relevant areas, topics and issues and creation of the suitable solution and recommendations for the specific M&A department
A new strategy for MS/MS data acquisition applying multiple data dependent experiments on Orbitrap mass spectrometers in non-targeted metabolomic applications
No full textThe annotation and identification of metabolites is a current bottleneck in non-targeted metabolomics studies. Although accurate measurement of m/z is applied routinely in UPLC–MS applications to putatively annotate metabolites, the acquisition of MS/MS or MSn data is required to deduce structural information, further reducing the chemical search-space and providing greater confidence in metabolite annotation by comparison to mass spectral databases. Here we propose an innovative new strategy for data dependent acquisition (DDA) for on-line MS/MS data acquisition in parallel to full-scan metabolite profiling on LTQ-Orbitrap mass spectrometers. We recommend the application of different and integrated DDA MS/MS experiments to increase the coverage of unique metabolites for which MS/MS data were acquired. We demonstrate, for the first time, that the integrated application of both CID and higher-energy collisional dissociation ion activation methods, multiple different activation energies and narrow precursor ion m/z ranges of 100 or 300 for acquisition of MS/MS spectra provide complementary information and increases the number of unique metabolites for which MS/MS data is acquired. The strategies herein provide a different approach for data acquisition to increase the number of unique MS/MS mass spectra acquired for metabolite annotation purposes in non-targeted metabolomics studies. MS/MS data are available on request from the corresponding author
Engaging the manuscript: new editions and reading the 'whole book' in Chetham's Library MS 8009
No full textThis thesis considers the intersection of the manuscript and its literature through an examination of the late fifteenth century manuscript, Chetham’s Library 8009 (Mun. A.6.31) and provides four diplomatic editions. This manuscript contains fourteen texts in Middle English including romance, hagiography, courtesy literature, and a comic text. This thesis argues for the importance of reading medieval literature in its manuscript context. Although there is a growing trend to consider the ‘whole book’ and integrate analysis of the material artefact with interpretation, much work remains to be done.
In Part I, this thesis presents a new paradigm for reading medieval literature, and argues that the manuscript forms a very literal community of texts, and that each text acts as a co-creator of meaning with the others. It then demonstrates four brief contextual readings that may be made within Chetham 8009 across generic boundaries, and that produce a shift in interpretive focus .
Part II provides four diplomatic editions from Chetham 8009: the Life of St Katherine, the Liber Catonis, John Russell’s Book of Carving and Nurture, and the Book of the Duke and Emperor.
This thesis aims to contribute to the study of medieval literature by arguing for a methodological shift in the way the literature is approached and by providing access to four texts either previously unedited or not easily accessible
Confirmation of fructans biosynthesized in vitro from [1-C-13]glucose in asparagus tissues using MALDI-TOF MS and ESI-MS
No full textAccumulation of fructans was confirmed in asparagus tissues that had been cultured for 2 days on media supplemented with glucose. It is very common that fructans are biosynthesized from sucrose. We hypothesized however that fructans could also be biosynthesized from glucose. Stem tissues of in vitro-cultured asparagus were subcultured for 72 h on a medium containing 0.5 M of [1-C-13]glucose. A medium containing 0.5 M of normal (C-12) glucose was used as control. Carbohydrates were extracted from the tissues and analyzed using HPLC, MALDI-TOF MS and ESI-MS. HPLC results indicated that the accumulation of short-chain fructans was similar in both C-13-labelled and control samples. Short-chain fructans of DP =3-7 were detected using MALDI-TOF MS. The molecular mass of each oligomer in the C-13-labelled sample was higher than the mass of the natural sample by 1 m/z unit per sugar moiety. The results of ESI-MS on the HPLC fractions of neokestose and 1-kestose showed that these oligomers (DP =3) were biosynthesized from exogenous glucose added to the medium. We conclude that not only exogenous sucrose but glucose can induce fructan biosynthesis; fructans of both inulin type and inulin neoseries are also biosynthesized from glucose accumulated in asparagus tissues; the glucose molecules (or its metabolic products) were incorporated into fructans as structural monomers. (C) 2013 Elsevier GmbH. All rights reserved
Valdosta Project Change, Scrapbook, December 1998
No full textValdosta Project Change. “Valdosta Project Change Scrapbook, December 1998.” Lowndes County Historical Society. Valdosta State University Archives and Special Collections, MS-181: Valdosta Project Change scrapbook Collection, 1998-2003. 1 PDF document and scans, 41 pages. 840 MB (881,697,568 bytes).12/1- School counselor complains about parents- Yvonne Fournier; 12/2- Williams’ death inquest held today: Public expected to observe ‘utmost decorum’ during inquest of Williams death- Jodi M. Scott; 12/2- Sister denies brother’s jail death was ‘suspicious’: Autopsy shows Williard McFarland died in 1994 of natural causes- Jodi M. Scott; 12/2- Church member doesn’t like using term ‘reverend’- Robert L. Scott, Lake Park ; 12/2- People’s Tribunal calls for economic ‘reciprocity’- Mailbox Post Staff; 12/4- Inquest jurors split on racial lines: Coroner incorrectly reported the verdict of the inquest as unanimous- Jodi M. Scott; 12/4- Coroner’s inquest efforts undermined by mistake- no author; 12/4- Williams speaks out against racism- no author; 12/5- Finding truth remains goal in Williams case- no author; 12/7- ‘Granny’s Place’ offers help for kids- Jodi M. Scott; 12/9- Coroner’s jury returns accidental death- Suzanne Harris; 12/9- Coroner’s inquest did its job- no author; 12/13- Local businesses deal with People’s Tribunal- Lowell Vickers Jr. ; 12/13- Protest group prepares list: Businesses asked for money, support- Lowell Vickers Jr.; 12/15- Tribunal incorporated as nonprofit group: Organization approved for incorporation last month- Jodi M. Scott; 12/16- People’s Tribunal wanting ‘Cooperative Reciprocity’- Suzanne Harris; 12/16- Inquest doesn’t answer all question, dispel all doubts- Charles Moore; 12/17- Evidence in Willie Williams’ death reaches grand jury: District attorney mum on details of testimony- Jodi M. Scott; 12/17- Give us the list- no author; 12/17- Truth is Out- no author; 12/17- Double standard- no author; 12/17- Don’t understand- no author; 12/17- Whites help blacks- no author; 12/18- Civil rights leader praises work of People’s Tribunal- Joe Dunlop; 12/19- Deputy ‘no billed’ in inmate’s death: No evidence found of beatings, abuse- Jodi M. Scott; 12/21- Tribunal letter causes concern: Officials ‘tense’ about group’s plan asking for support- Shelby G. Spires ; 12/23- Examine Procedures Now- no author; 12/30- Sheriff plans no change in arrest procedures- Suzanne Harris
Valdosta Project Change, Scrapbook, May 1998
No full textValdosta Project Change. “Valdosta Project Change Scrapbook, May 1998,” Lowndes County Historical Society. Valdosta State University Archives and Special Collections, MS-181: Valdosta Project Change Collection, 1997-2023. 1 PDF document and scans, 12 pages. 210 MB (221,104,354 bytes).5/11- Racial discrimination at law school- Nat Hentoff; 5/19- Project Change awards- no author ; 5/20- Council to hear concerns over retention pond plans- Jodi M. Scott; 5/20- Former governor’s racial attitude being debated- Associated Press; 5/22- State law may allow teachers to bear arms- no author; 5/24- Rep. John Lewis recalls struggle civil rights- Associated Press; 5/26- City’s project targets crime: Director hopes Weed and Seed improves safety- Jodi M. Scott
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