31 research outputs found
Symposium on Buddhist Perspectives on Free Will: Agentless Agency?
No full textThis special issue of the Journal of Buddhist Ethics, Volume 25, is a symposium on the anthology, Buddhist Perspectives on Free Will: Agentless Agency? (Repetti), and on the topic reflected by that title, more broadly, based on an Author Meets Critics session of the 2018 American Philosophical Association Eastern Division meeting organized by Christian Coseru. To orient readers new to the topic, I first sketch what some of the issues are regarding Buddhist perspectives on free will. Second, I briefly describe the anthology, and third, I introduce the several contributions to this symposium. As I am sympathetic to most of the papers here, I only respond briefly to them in this introduction, giving some reasons for my approval. Two papers here, however, are significantly critical of either the anthology as a whole (Brent), or critical of my contributions to it (Meyers). I respond separately to each of them in the last two papers in the symposium. Together with this introduction, all the included papers are original
Multivariate optimization of the extraction and cleaning procedure to determine neonicotinoids in honey from intensive livestock and agricultural production areas by UHPLC-MS/MS
No full textNeonicotinoid insecticides have been associated with the decline of bee population. This study aimed to investigate the occurrence of neonicotinoids in honey samples from apiaries located in areas of intensive livestock and agricultural production in Argentina. For this purpose, a QuEChERS based analytical method was developed and optimized using multivariate strategies to reduce the processing time and the necessary amount of salts and sorbents. Validation was based on SANTE 11312/2021 guidelines, with recovery percentages between 79 and 120 %, and limits of quantitation between 0.25 and 1.00 µg kg− 1. The present study provides a highly sensitive and reliable method for ascertaining residue levels of eight insecticides in 151 honey samples using liquid chromatography coupled to mass spectrometry (UHPLC-MS/MS). The presence of three of the target analytes was determined in 13 % of the samples, indicating that honey bee colonies were exposed to neonicotinoids, especially imidacloprid and thiamethoxam. The maximum residue limits were not exceeded in any of the samples; therefore, there is no risk to consumers from the public health point of view.Fil: Michlig, Melina Paola. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Brasca, Romina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Demonte, Luisina Delma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Magni, Florencia Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentin
Implementazione di una piattaforma di business intelligence basata sul prodotto Oracle BI Apps
No full textL'elaborato riguarda la descrizione di un progetto di business intelligence, scaturito dall’esigenza dell’azienda cliente di sfruttare al meglio il proprio sistema informativo. Per la realizzazione del progetto è stato utilizzato il prodotto Oracle Business intelligence Application, sul quale sono state effettuate delle personalizzazioni atte a soddisfare i requisiti concordati. Verranno illustrate le fasi del progetto, come la definizione degli obiettivi, le attività di analisi e sviluppo, con particolare attenzione alla realizzazione di alcune aree funzionali. Infine verrà descritta la reportistica implementata e verranno illustrate alcune considerazioni sullo svolgimento e sui risultati dell’attività di progetto, seguita dalla presentazione dei possibili sviluppi futuri
Earthworm injury test for in-situ biomonitoring of pesticides in biobeds
No full textBiobeds are presented as an alternative for good pesticide wastewater managementon farms. This work proposes a new test for in-situ biomonitoring of pesticidedetoxification in biobeds. It is based on the assessment of visually appreciable injuriesto Eisenia fetida. The severity of the injury to each exposed individual is assessed fromthe morphological changes observed in comparison with the patterns established inseven categories and, an injury index is calculated. A linear relationship between theproposed injury index and the pesticide concentration was determined for eachpesticide sprayed individually in the biomixture. The five pesticides used were atrazine,prometryn, clethodim, haloxyfop-P-methyl and dicamba. In addition, a multiple linearregression model (i.e., a multivariate response surface) was fitted, which showed agood generalization capacity. The sensitivity range of the injury test was tested from0.01 to 630 mg kg-1 as the total pesticide concentration. This index is then used tomonitor the detoxification of these pesticides in a biomixture (composed of wheatstubble, river waste, and soil, 50:25:25% by volume) over 210 days. The results arecompared with standardized tests (Eisenia fetida avoidance test and Lactuca sativaseed germination test) carried out on the same biomixture. The results are alsocompared with data on the removal of pesticides. The injury test showed a bettercorrelation with the removal of pesticides than the avoidance test and seedgermination test. This simple and inexpensive test has proved to be useful fordecontamination in-situ monitoring in biobeds.Fil: Lammertyn, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Magni, Florencia Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaFil: Duran, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaFil: Godoy, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Zalazar, Cristina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentin
Dietary Supplementation of Hericium erinaceus Increases Mossy Fiber-CA3 Hippocampal Neurotransmission and Recognition Memory in Wild-Type Mice
No full textHericium erinaceus (Bull.) Pers. is a medicinal mushroom capable of inducing a large number of modulatory effects on human
physiology ranging from the strengthening of the immune system to the improvement of cognitive functions. In mice, dietary
supplementation with H. erinaceus prevents the impairment of spatial short-term and visual recognition memory in an Alzheimer
model. Intriguingly other neurobiological effects have recently been reported like the effect on neurite outgrowth and differentiation
in PC12 cells. Until now no investigations have been conducted to assess the impact of this dietary supplementation on brain
function in healthy subjects. Therefore, we have faced the problem by considering the effect on cognitive skills and on hippocampal
neurotransmission in wild-type mice. In wild-type mice the oral supplementation with H. erinaceus induces, in behaviour test, a
significant improvement in the recognition memory and, in hippocampal slices, an increase in spontaneous and evoked excitatory
synaptic current in mossy fiber-CA3 synapse. In conclusion, we have produced a series of findings in support of the concept that
H. erinaceus induces a boost effect onto neuronal functions also in nonpathological conditions
Case report Open Access Liver abscess caused by Klebsiella pneumoniae: two case reports
No full textThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licens
Gain of hTERC: A genetic marker of malignancy in oral potentially malignant lesions
No full textSummary Oral squamous cell carcinoma (OSCC) is the most common oral cancer, and major efforts is being made to identify molecular markers capable to differentiate oral potentially malignant lesions (OPMLs) with indolent course from lesions with aggressive behavior. We undertook a study to evaluate if gain of the human telomerase RNA component (hTERC) gene in OPMLs could indicate lesions at high risk of developing OSCC. The study was performed on 30 OPMLs with long-term follow-up using a dual-color interphase fluorescence in situ hybridization (FISH) for hTERC status. Progression to malignancy was observed in 9 of 10 cases harboring hTERC gain and in 1 of 20 cases retaining a normal copy number of hTERC (P <.0001). Combining morphological grading and FISH analysis, all the cases with high-grade squamous intraepithelial lesion or carcinoma in situ harboring hTERC amplification progressed to OSCC, whereas none of the low-grade squamous intraepithelial lesions without hTERC gain progressed. Intermediate situations occurred. The data suggest that precise morphological evaluation together with FISH assessment for hTERC gain might pave the way to stratify OPMLs into high-risk and low-risk categories and could be helpful in selecting the most appropriate treatment
Determinación de glifosato, ácido aminometilfosfónico y glufosinato en miel por cromatografía líquida acoplada a espectrometría de masa
No full textArgentina ha transformado su agricultura basada en importantes innovaciones tecnológicas, lo que incluyó la adopción de cultivos genéticamente modificados diseñados para ser resistentes a herbicidas específicos, principalmente glifosato. Este herbicida, ampliamente utilizado en la agricultura de nuestro país, aplicado en diversos cultivos, está en la mira de la sociedad generando gran preocupación por sus efectos negativos en la salud humana y el ambiente. Si bien sus posibles efectos, comportamiento y destino final ambiental resultan controversiales, es necesario realizar estudios específicos y controles periódicos. Las características fisicoquímicas de estos compuestos (polares, anfóteros con varios valores de pKa, bajo peso molecular) introducen problemas específicos en las etapas de separación y detección. La derivatización con 9-fluorenilmetilcloroformato (FMOC-Cl) sigue siendo una opción valiosa debido a que presenta ciertas ventajas (uso de cromatografía de fase reversa, mejora de la retención en cromatografía líquida, aumenta el peso molecular, la sensibilidad, la reproducibilidad de la reacción, y se obtienen límites de cuantificación muy competitivos) incluso cuando se aplica a muestras complejas como la miel de abeja. En este trabajo se presenta un método simple para la cuantificación de glifosato, su principal metabolito, ácido aminometilfosfónico (AMPA) y glufosinato en muestras de miel basado en derivatización con FMOC-Cl y análisis mediante cromatografía líquida acoplada a espectrometría de masa en tándem. La metodología implica una etapa de extracción con agua seguida de la reacción de derivatización y finalmente limpieza de los extractos mediante partición líquido-líquido con diclorometano. En el proceso se optimizó la etapa de extracción, los reactivos utilizados, el tiempo de reacción, valores de pH óptimos y etapas de limpieza de los extractos. Se han obtenido resultados satisfactorios que demuestran que este método es simple y reproducible, con desviaciones estándares relativas por debajo del 20% y porcentajes de recuperación entre 70-120%, alcanzando un límite de cuantificación de 5 µg/kg.Fil: Demonte, Luisina Delma. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Michlig, Melina Paola. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Michlig, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Magni, Florencia Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaCongreso Argentino de Apicultura 2021ArgentinaSociedad Argentina de Apicultore
Determination of pesticides and mycotoxins in feed: Applicability of an analytical method in routine analysis
No full textIn routine analysis it is often necessary to introduce minor changes in protocols or extend the applicability of previously validated methods to similar matrices. The SANTE guidelines contemplate on-going validation as a quality control process to verify analytical performance through a reduced number of experiments1.In this work, a validated QuEChERS method for the determination of pesticides and mycotoxins in corn and alfalfa was used for the analysis of a large number of different types of feed samples (cereals, pastures, silages). Briefly, 2 g of sample were hydrated with 10 ml H2O (2% CH2O2), followed by extraction and partition with 10 ml acetonitrile, MgSO4/NaCl (4:1). An aliquot of the extract was then cleaned-up using d-SPE with MgSO4, C18 and PSA, prior to analysis by UHPLC-MS/MS2.A modification of the clean-up step had to be introduced due to the large number of samples to be processed and availability of sorbent salts at the time of analysis. Thus, for cereal-based samples only MgSO4/C18 was used while MgSO4/PSA was used for pasture-based samples.Following the concepts of the on-going validation, the mean recovery (Rec%) was evaluated at the same concentration level from validation (100 µg/kg). Precision was evaluated as repeatability and intermediate precision, repeating the test 2 and 3 consecutive days for corn and alfalfa, respectively. The recovery results obtained after the modifications were compared with those from the initial validation through statistical t-tests. Finally, an additional quality control test was done by applying the methodology to a European Union Proficiency Test (EUPT CF18-2014) in wheat flour.As a result, 80% of the studied compounds (117 pesticides and 2 mycotoxins) showed recoveries in the 60-140% range accepted for routine analysis by the SANTE guide, with RSDs <20% in both matrices.From the statistical tests, the Rec% of more than 85% of the studied compounds were not affected by the changes introduced. Moreover, for compounds such as acephate, chlorpyriphos methyl, clethodim, fenthion and metribuzin in corn, and methomyl, metribuzin, thiabendazole, profenofos, spinosad D and terbuphos in alfalfa their Rec% improved with respect to the initial validation. Finally, the results from the EUPT test were compared to the declared concentration through a z-score, verifying the aptitude of the method for the determination of azoxystrobin, epoxiconazole, linuron,pyraclostrobin and carbendazim.Fil: Magni, Florencia Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Michlig, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Michlig, Melina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina8th Latin American Pesticide Residue WorkshopPanamáPanamáSociedad Latinoamericana sobre Residuos de PlaguicidasUniversidad Nacional del LitoralGobierno de la República de Panamá. Ministerio de Desarrollo Agropecuari
Determination of glyphosate, AMPA and glufosinate in honey: Comparative tests on the sample preparation stage
No full textBeekeeping is an activity of great economic and eco-systemic importance, and Argentina is a major country in the world in this activity. Regarding the cleaning and safety of honey, residues and chemical contaminants´ unwanted presence is of great concern, both by residues originated in the treatments applied to the hive and by the use of agrochemicals in agriculture that potentially contaminate the beekeeping chain. To meet today´s quality and safety requirements is necessary to realize more pesticide control analysis.Argentina, The United States and Brazil use 80% of glyphosate´s world total. In our country since 1996 has been applied to various transgenic crops headed by soy, with a significant annual load (200 thousand tons). Therefore, the main objective was to develop an analytical methodology to determine glyphosate, AMPA and glufosinate in honey and related matrices such as nectar. Based on the pre-column derivatization strategy with FMOC-Cl and analysis by UHPLC-MS/MS. Different alternatives were evaluated and adapted to the objective. For methodology optimization: extraction solvents, volume and concentration of the derivatizing reagent, volume and concentration of the borate buffer, different reagents to achieve the optimum pH (pH 9), reaction time and cleaning alternatives of the extracts also were evaluated (L-L partition and SPE).Recovery tests at two concentration levels (10 and 100 ug kg-1) in triplicate were performed to evaluate the methodology. First, honey samples weighed in 50 mL centrifuge tubes were placed in a thermostated bath at 40-50°C for 5 min to achieve fluidity. They were then spiked with the standard mixture (glyphosate, AMPA and glufosinate) and vortexed to homogenize. After that, the extraction, derivatization and cleaning stage were performed for each of the evaluated alternatives. The final methodology involves an extraction step with water and mechanical agitation for 30 min followed by the derivatization reaction and finally L-L partition with dichloromethane as a clean-up stage1. Satisfactory results have been obtained, showing that this method is consistent and reliable, with RSDs < 20% and recoveries between 70-105% at 5, 10, 25 and 50 ug kg-1.Also, together with the optimization of the sample preparation, to achieve a better response of the analytes as a whole, the instrumental conditions were optimized based on experiments´ statistical designs to increase detection sensitivity to obtain low detection and quantification limits.In this way, it was possible to achieve an acceptable analytical method for determining glyphosate, AMPA and glufosinate that was applied to analyze raw and commercial honey samples.Fil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Magni, Florencia Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Michlig, Melina Paola. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina8th Latin American Pesticide Residue WorkshopPanamáPanamáUniversidad Nacional del LitoralGobierno de la República de Panamá. Ministerio de Desarrollo AgropecuarioSociedad Latinoamericana sobre Residuos de Plaguicida
