1,721,181 research outputs found
Valutazione dell'utilizzo dei bisfosfonati in neoplasie solide mediante studio di espressione genica
No full textNegli ultimi anni si è assistito ad una notevole ricerca di marcatori di espressione genica nelle cellule tumorali finalizzata ad applicazioni diagnostiche e per monitorare le diverse terapie. Un target molto importante oggetto di diversi studi in vivo e in vitro è rappresentato dall’enzima telomerasi. In particolare, lo studio di espressione genica dei suoi componenti è apparso molto utile per rivelare cellule tumorali circolanti nel sangue periferico e per evidenziare quindi la presenza di micrometastasi.
La presenza di metastasi scheletriche in pazienti oncologici è gravata da una serie di complicanze. I bisfosfonati sono una classe di farmaci con una potente attività di inibizione del riassorbimento osseo e vengono sempre più utilizzati nel trattamento di pazienti con metastasi ossee. Alcuni studi clinici hanno dimostrato che tali farmaci sono molto efficaci nel trattamento delle metastasi ossee di tumori prostatici e mammari. Recenti studi hanno inoltre rivelato che i bisfosfonati sono anche in grado di agire direttamente sulla cellula tumorale interferendo con la crescita e la sopravvivenza delle cellule metastatiche nell’osso.
La presente tesi illustra i risultati relativi alla downregolazione dell’espressione dei geni della telomerasi utilizzando i bisfosfonati in vitro, su linee tumorali, ed in vivo, su pazienti neoplastici affetti da carcinoma prostatico e mammario.
Relativamente agli studi in vitro, verranno presentati i dati di inibizione cellulare mediante test di vitalità sulle colture cellulari trattate con concentrazioni crescenti di diversi bisfosfonati e verrà analizzata la relativa espressione dei geni della telomerasi mediante la tecnica di RT Real Time PCR. Verranno inoltre valutati gli effetti dello zoledronato su linee prostatiche co-incubate con il geranylgeraniol che è noto ripristinare la via del mevalonato.
Per quanto riguarda gli studi in vivo, saranno presentati i dati relativi all’espressione dei geni della telomerasi nei sieri dei pazienti oncologici trattati con lo zoledronato.
La presente esperienza ha dimostrato che:
• I geni della telomerasi rappresentano un target alternativo all’azione dei bisfosfonati sulle cellule tumorali;
• Gli amino-bisfosfonati riducono la proliferazione nelle cellule tumorali mediante la down-regolazione dell’espressione dei geni della telomerasi;
• I bisfosfonati agiscono sull’espressione genica in una maniera concentrazione-dipendente;
• L’utilizzo dello zoledronato in vivo è in grado di abbassare i livelli di espressione dei geni della telomerasi;
• L’utilizzo di tale target molecolare in vivo è un valido metodo per monitorare l’azione del trattamento con zoledronato in pazienti neoplastici.Non disponibil
Valutazione dei risultati ottenuti nela raccolta e nella manipolazione di cellule staminali ematopoietiche periferiche per autotrapianto in neoplasie solide ed ematologiche
No full texttesi di specializzazione in biochimica e chimica clinic
[Bone biopsy in the Chronic Kidney Disease (CKD)]
No full textHistomorphometry or quantitative histology is the analysis on histologic sections of bone resorption, formation and structure parameters. It is the only technique allowing a dynamic evaluation of osteoblast activity after labelling with tetracycline. In addition, the use of computed image analyzer allows the possibility to assess bone microarchitecture. Histomorphometric bone biopsy is a reliable and well-tolerated procedure. Bone samples are taken at iliac crest level under local anesthesia and sedation. Samples are put into methyl-metacrilate resin where the sections are prepared for the microscopic analysis of different histomorphometric parameters. Histomorphometry is used to exclude or confirm the diagnosis of osteomalacia and also to evaluate bone damage associated with particular treatments (for example, glucocorticoids, anticonvulsants) or in case of rare bone diseases (osteogenesis imperfecta). Recently, this approach has been repurposed for the evaluation of bone disease during Chronic Kidney Disease, particularly to differentiate high and low bone turnover osteodystrophy. Kidney Disease Improving Global Outcomes (KDIGO) guidelines have pointed out the situations requiring bone biopsy for histomorphometric analysis. New techniques using molecular biology will improve the accuracy and predictive value of this approach
Techno-economic design of a smart multienergy microgrid
No full textThe paper presents the main objectives, approach, and research challenges of the Mission Innovation project 'MISSION Multivector Integrated Smart Systems and Intelligent microgrids for accelerating the energy transition' carried out in the research center of Portici (ENEA). More specifically, the work started with an analysis of the state of the art of smart networks implemented at national and international level, with the aim of identifying the innovative elements of Smart Energy Microgrid (SEM) ENEA compared to the existing installations. A special focus has been given to the preliminary assessment of the technologies involved, the sizing and the constraints of the SEM. The productions of the technologies that allow to understand the behavior of the entire simulated energy system are reported. Based on the analysis of the results achieved and through the considerations of the available space, the most feasible scenario is revealed
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Valenti, Maria
No full textCentro Asturiano membership record of Maria Valenti; Socio Number: 999.https://digitalcommons.usf.edu/asturiano_membership/5955/thumbnail.jp
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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