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    Growth response of juvenile dentex (Dentex dentex, L.) to varying protein level and protein to lipid in practical diets

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    The dentex (Dentex dentex L.) is a fast-growing sparid which represents a possible candidate for Mediterranean mariculture. As the basic nutrient requirements of this species are unknown, two feeding trials were carried out to identify suitable protein and lipid (energy) levels to be used in formulating practical diets for dentex. The experiments used groups of 30 specimens (each fish weighing 17 or 20.7 g) which were kept in 12 flow-through 160 1 tanks supplied with seawater at 21 min(-1) (temperature 20 +/- 1 degrees C; salinity 33 ppt). An initial 4-week trial was conducted to obtain a preliminary estimate of the optimal protein level. Triplicate groups were fed four practical diets with increasing levels of protein (44.3, 49.3, 55.7 and 58.9% dry matter (DM), over 90% of which was supplied by white fish meal) and a constant lipid content (17.3% DM; mostly supplied by fish lipids) to satiation. An analysis of the nutrient-weight gain relationship indicated that the optimal dietary protein level was 49.3% DM. In a second 60 day experiment, duplicate groups of fish were fed six diets with three protein levels (44.4, 49.5 and 55.8% DM) and two lipid contents (12.0 and 17.3% DM) within each protein level, nearly to satiation. Growth performance was lowest in fish given diets containing 44.4 or 49.5% protein and 12% lipid and was improved (P < 0.05) either with diets supplying 55.8% protein (regardless of dietary lipid)or 49.3% protein and 17.4% lipid. The results observed in the second trial suggest that when protein efficiency or retention are considered even dietary levels of 44.3% protein and 17.2% lipid (i.e. 24.3 g protein kJ(-1) calculated available energy) could be assumed as suitable levels for formulating practical diets for this species, providing high quality fish meal and oil are used

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Evaluation of mast cells in calf and heifer uteri

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    Little is known about the characteristics of mast cells present in the wall of the bovine uterus. The aims of the present study were to determine the main distribution patterns of mast cells residing in the uterine walls of calves and heifers and to obtain reference values for these cells in order to assist in the evaluation of endometrial biopsies in cows. A total of 59 young Italian Friesian cattle (30 prepubertal heifers 8 to 10 mo old and 29 post pubertal heifers 15 to 18 mo old that had not calved) were chosen on the basis of absence of lesions of the genital tract, following clinical examination for macroscopic and microscopic pathology. Only pubescent heifers which were in the diestral phase of their cycle were chosen. After slaughter, tissue samples were collected from the uterine body and each horn, and were then fixed in buffered formalin. Histological sections (5 μm), stained with Toluidine blue (0.003% at pH 4.9), were used for cell counts in 5 optical fields at x 400 magnification (0.18 mm2), randomize for each layer from both the wall of the body and each born: stratum compactum (SC), st. spongiosum (SS), st. musculare (SM), st. vasculare (SV) and tunica serosa (TS). The number of mast cells in the layers for pre- and post-pubertal heifers were, respectively: SC 5.5 and 11.2; SS 2.8 and 2.7; SM 0.8 and 2.8; SV 3.0 and 4.6; and TS 1.4 and 3.9. The results indicated that the number of mast cells increased with age (calves 2.7 vs heifers 4.7; P<0.001), particularly in heifers, that the values for the uterine body and horns were not different (4.5 and 4.8) and that SC and SV had the highest values in both groups. The reference values proposed for the resident mast cells are (mast cells/ x 400): 11 for SC alone, 7 as an average of SC+SS, and 5 as an average of SC+SS+SM+SV
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