1,721,138 research outputs found
Two dimensional liquid chromatography technique coupled with mass spectrometry analysis to compare the proteomic response to cadmium stress in poplar.
No full textPoster Communication Abstract 2B.0
The proteomics of heavy metal hyperaccumulation by plants
No full textHyperaccumulators are distinguished from non-hyperaccumulators on the basis of their capacity to extract heavy metal ions from the soil, their more efficient root-to-shoot translocation of these ions and their greater ability to detoxify and sequester heavy metals in the shoot. The understanding of the mechanisms underlying metal ion accumulation has progressed beyond the relevant biochemistry and physiology to encompass the genetic and molecular regulatory systems which differentiate hyperaccumulators from non-hyperaccumulators. This paper reviews the literature surrounding the application of proteomics technology to plant metal hyperaccumulation, in particular involving the elements As, Cd, Cu, Ni, Pb and Zn. The hyperaccumulation process across a number of unrelated plant species appears to be associatedwith proteins involved in energy metabolism, the oxidative stress response and abiotic and biotic stress. The relevance of transducers of the metal stress response to the phenomenon of hyperaccumulation is summarized. Proteomic data complement the more voluminous genomic and transcriptomic data sets in providing a more nuanced picture of the process, and should therefore help in the identification of the major genetic determinants of the hyperaccumulation phenomenon
Molecular, physiological and physical analysis of caesium resistant Arabidopsis mutants.
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Noccaea caerulescens populations adapted to grow in metalliferous and non-metalliferous soils: Ni tolerance, accumulation and expression analysis of genes involved in metal homeostasis
No full textThree populations of the Brassicaceae Noccaea caerulescens: i) the metallicolous Mt. Prinzera population (Italy), which have adapted to grow on serpentinite, a soil naturally rich in Ni, Co, Cr; ii) the metallicolous La Calamine population (Belgium), which have adapted to growth on soil highly contaminated by Cd, Zn and Pb, and iii) the non-metallicolous population growing near the town of Rožnov pod Radhoštěm (Czech Republic), were grown in hydroponics and treated with different Ni concentrations (0, 10 and 100 uM NiSO4). Ni tolerance and accumulation were analysed along with the expression of genes belonging to different families involved in plant metal homeostasis: ZNT1, ZNT2, NRAMP3, NRAMP4 coding for non-ATP-hydrolysing plasma membrane and vacuolar metal transporters, HMA3, HMA4 coding for ATP-hydrolising metal transporters, NAS1, NAS3, NAS4 and MT1B involved in metal chelation. The three populations showed different levels of expression of some of the tested genes in condition of 0 uM Ni. In addition, the Ni hyperaccumulator Mt. Prinzera showed the highest Ni translocation capacity at 10 uM Ni and a specific up-regulation of ZNT1, ZNT2, NAS3, NRAMP3 and NRAMP4 genes. With the same Ni treatment, the La Calamine population induced HMA4 and MT1B genes, while the Rožnov pod Radhoštěm population only displayed an over-expression of all the genes at 100 uM Ni, the condition in which this population suffers heavily from Ni stress. The expression of ZNT1, ZNT2, NRAMP3, NRAMP4 was also tested on N. caerulescens plants growing in the natural environment on Mt. Prinzera. Higher transcript levels of ZNT1 and ZNT2 were associated with higher levels of plant’s total Ni content. Overall these results suggested that some of the genes considered can have a role in determining metal tolerance and accumulation in metallicolous N. caerulescens populations, while they were not involved in metal homeostasis in the non-metallicolous population
Smart agriculture for food quality: facing climate change in the 21st century
Full text linkClimate change, with increasing temperatures and atmospheric carbon dioxide levels, constitutes a severe threat to the environment and all living organisms. In particular, numerous studies suggest severe consequences for the health of crop plants, affecting both the productivity and quality of raw material destined to the food industry. Of particular concern is the reduction of proteins and essential micronutrients as iron and zinc in crops. Fighting this alarming trends is the challenge of Climate-Smart Agriculture with the double goal of reducing environmental impacts (use of pesticides, nitrogen and phosphorus leaching, soil erosion, water depletion and contamination) and improving raw material and consequently food quality. Organic farming, biofertilizers and to a lesser extent nano-carriers, improve the antioxidant properties of fruits, but the data about proteins and micronutrients are rather contradictory. On the other hand, advanced devices and Precision Agriculture allow the cultivations to be more profitable, efficient, contributing more and more to reduce pest diseases and to increase the quality of agricultural products and food safety. Thus, nowadays adoption of technologies applied to sustainable farming systems is a challenging and dynamic issue for facing negative trends due to environmental impacts and climate changes
In vitro and in silico analysis of two genes (A2-1 and G1-1) differentially regulated during dormancy and sprouting in potato tubers
No full textDifferential display-mediated isolation of a genomic sequence for a putative mitochondrial LMW HSP specifically expressed in condition of induced thermotolerance in Arabidopsis thaliana (L.) Heynh.
No full textMetodologie genetico-molecolari per l'individuazione e l'isolamento di geni coinvolti nella termotolleranza in Arabidopsis thaliana.
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