1,722,150 research outputs found
NADH fluorescence lifetime is an endogenous reporter of α-synuclein aggregation in live cells.
Full text linkα-Synuclein (aS) aggregation has been amply investigated for its involvement in Parkinson's disease because its amyloid fibrils are the main constituent of Lewy bodies, one of the hallmarks of the disease. aS aggregation was studied here in vitro and in cellular models to correlate aggregation products with toxicity mechanisms. Independent results published elsewhere suggested that aS overexpression and/or aggregation may impair cellular metabolism and cause mitochondrial damage. In this context, we report the characterization of changes in NADH fluorescence properties in vitro and in human embryonic kidney 293 cells upon aS aggregation. The application of the phasor approach to study NADH fluorescence lifetime and emission allowed us to identify changes that correlate with aS aggregation. In particular, the fraction of bound NADH, characterized by longer lifetimes in comparison to free NADH, is increased, and the maximum of the NADH emission is shifted toward shorter wavelengths in the presence of aggregating aS both in vitro and in cells. These data suggest that NADH binds to aggregated aS. NMR experiments in vitro substantiate such binding, which occurs during aggregation. NADH fluorescence is thus useful to detect aS aggregation and by extension the associated oxidative stress.-Plotegher, N., Stringari, C., Jahid, S., Veronesi, M., Girotto, S., Gratton, E., and Bubacco, L. NADH fluorescence lifetime is an endogenous reporter of α-synuclein aggregation in live cells
Improving lambda tuning of PI controllers for load disturbance rejection
No full textIn this paper we propose a tuning rule for PI controllers that is based on the same rationale of lambda-tuning but aims to optimize the performance in the load disturbance rejection task. In particular, the maximum sensitivity obtained by lambda-tuning is used as a constraint in minimizing the integral of the absolute error, in such away to achieve the desired tradeoff between performance and robustness. The tuning rule has been obtained by finding the optimal parameters for different processes by means of genetic algorithms and then by fitting the results with appropriate functions
THE EFFECTS OF ALFAPROSTOL (PGF2a analogue) AND eCG ON REPRODUCTIVE PERFORMANCES IN POSTPARTUM RABBITS
No full text[EN] The aim of this study was to verify the effectiveness of post partum pharmacological oestrus synchronisation with alfaprostol or eCG in improving rabbit doe reproductive performances and to evaluate the incidence of pseudopregnancy in this species. One hundred and eighty commercial hybrid rabbit does, multiparous and lactating, were randomly divided into three groups (n=60) and treated on day 8 post partum (pp) as follows: Group A, 1 mL s.c. vitaminic solution with 200 mg alfaprostol, a synthetic analogue of PGF2a; Group B, 1 mL sc vitaminic solution with 20 I.U eCG; Group C (Control) 1 mL sc vitaminic solution. On day 11 pp (3 days after treatment), all the rabbits were artificially inseminated (AI), and injected with 0.8 mg of buserelin i.m. to induce ovulation. Concurrently, blood samples were taken for progesterone (P4) analysis by RIA with the following schedule: day 8 pp (before treatment), day 11 pp (before AI and the induction of ovulation) and day 16 pp (5 days after AI). Fertility was not influenced by hormonal treatments (71.7% and 71.2% respectively) compared to control (66.1%). Conversely, both PGF2a and eCG hormonal synchronisation treatments increased (P1 ng/mL). On day 5 after insemination 95.5% had P4 values, which attest to the presence of functional corpora lutea (CL) (P4>2 ng/mL). The results of this study show that as long as rabbitry is properly managed zootechnically and sanitarily, drugs such as alfaprostol and eCG, while not ameliorating fertility rate when used for post partum oestrus sychronisation, can increase litter size.Mollo, A.; Veronesi, M.; Battocchio, M.; Cairoli, F.; Brecchia, G.; Boiti, C. (2003). THE EFFECTS OF ALFAPROSTOL (PGF2a analogue) AND eCG ON REPRODUCTIVE PERFORMANCES IN POSTPARTUM RABBITS. World Rabbit Science. 11(2):63-74. https://doi.org/10.4995/wrs.2003.498SWORD637411
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Therapeutic indications for high dose intravenous immunoglobulin in autoimmune or immunomediated diseases in pediatrics [Indicazioni terapeutiche con Ig e.v. ad alte dosi in patologie autoimmuni o immunomediate in eta pediatrica]
No full textImpiego di differenziatori non lineari in algoritmi di tipo PID
No full textNell’ambito della regolazione basata sugli algoritmi di tipo PID (Proporzionale-Integrale-Derivativo) l’azione
derivativa, che pure potrebbe dare un prezioso contributo in termini di anticipo di fase, viene spesso disabilitata a causa della sua sensibilità al rumore sul segnale di misura che può causare un degrado delle prestazioni finanche
a risultare destabilizzante per il sistema in anello chiuso. Per ovviare all’inconveniente, accanto alle più o meno raffinate tecniche di filtraggio, può trovare posto anche l’impiego di differenziatori non lineari che, in luogo del
normale operatore di derivata, forniscono migliori prestazioni in termini di robustezza contro il rumore senza pagare un prezzo elevato in termini di sfasamento, come invece può accadere per i filtri. Scopo di questo contributo è quello di illustrare alcune di queste tecniche, valutarne le prestazioni e mostrare come possano essere facilmente implementate nei sistemi di controllo disponibili in commercio
- …
