1,721,043 research outputs found

    Genetic diversity and differentiation in European beech (Fagus sylvatica L.) stands differing in management history

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    The impact of forest management on genetic diversity and mating was examined in European beech (Fagus sylvatica L.). Ten beech stands located in Europe were studied in pair-wise plots, differing in management intensity. The stands were genotyped with four highly polymorphic microsatellite loci. Comparison for genetic diversity measures between the stands with limited management and the high management-intensity stands (mostly shelter wood system) revealed no significant differences for allelic richness (A), effective number of alleles (Ae), number of rare alleles (Arare), neither for observed (Ho) nor expected heterozygosity (He). In all stands a significant excess of homozygotes was found, which is in agreement with previous isozyme publications. However, the increase in the inbreeding coefficient (Fis) in the stands with limited management was significantly higher than in the highly managed stands. Expectedly a low, but significant, differentiation among all stands was found (Fst = 0.058) which still reveals a clear geographic structure. The results indicate that the shelter wood system has no or minimum impact on the genetic diversity in European beech

    Posidonia oceanica meadows of the Italian southern Adriatic Sea display different genetic structure

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    The Mediterranean-endemic seagrass Posidonia oceanica (L.) Delile forms meadows of great ecological importance that are currently under high anthropogenic pressures. We investigated the genetic structure of two meadows located in the Adriatic Sea along the coast of Italy. The San Vito-Barletta meadow is located in an unprotect area close to several medium-large towns suggesting that it is more subjected to a wide panel of anthropic impacts. This meadow displays subpopulation structure and higher genetic/genotypic diversity which may be the consequence of a combination of different anthropic impacts and environmental features. In contrast, the Tremiti Islands meadow is included within a marine protected area away from large cities. Genetic analysis showed that this meadow possesses a monoclonal structure with low genetic/genotypic diversity. Yet, our data indicate that both meadows require specific conservation/restoration policy

    A RAPD, AFLP and SSR linkage map, and QTL analysis in European beech (Fagus sylvatica L.)

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    The genetic linkage map of European beech (Fagus sylvatica L.) that we report here is the first to our knowledge. Based on a total of 312 markers (28 RAPDs, 274 AFLPs, 10 SSRs) scored in 143 individuals from a F1 full-sib family. Two maps (one for each parent) were constructed according to a “two-way pseudo-testcross” mapping strategy. In the male map 119 markers could be clustered in 11 major groups (971 cM), while in the female map 132 markers were distributed in 12 major linkage groups (844 cM). In addition, four and one minor linkage groups (doublets and triplets) were obtained for the male and female map respectively. The two maps cover about 82% and 78% of the genome. Based on the position of 15 AFLP and 2 SSR loci segregating in both parents, seven homologous linkage groups could be identified. In the same pedigree we investigated the association with genetic markers of several quantitative traits: leaf area, leaf number and shape in 2 different years, specific leaf area, leaf carbon-isotope discrimination and tree height. A composite interval-mapping approach was used to estimate the number of QTLs, the amount of variation explained by each of them, and their position on the genetic linkage maps. Eight QTLs associated with leaf traits were found that explained between 15% and 35% of the trait variation, five on the female map and three on the male map
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