352 research outputs found
Gephyrin regulates GABAergic and glutamatergic synaptic transmission in hippocampal cell cultures
No full textDevelopment of an Efficient Electroporation Method for Iturin A-Producing Bacillus subtilis ZK
No full textIn order to efficiently introduce DNA into B. subtilis ZK, which produces iturin A at a high level, we optimized seven electroporation conditions and explored an efficient electroporation method. Using the optimal conditions, the electroporation efficiency was improved to 1.03 x 10(7) transformants/g of DNA, an approximately 10,000-fold increase in electroporation efficiency. This efficiency is the highest electroporation efficiency for B. subtilis and enables the construction of a directed evolution library or the knockout of a gene in B. subtilis ZK for molecular genetics studies. In the optimization process, the combined effects of three types of wall-weakening agents were evaluated using a response surface methodology (RSM) design, which led to a two orders of magnitude increase in electroporation efficiency. To the best of our limited knowledge, this study provides the first demonstration of using an RSM design for optimization of the electroporation conditions for B. subtilis. To validate the electroporation efficiency, a case study was performed and a gene (rapC) was inactivated in B. subtilis ZK using a suicide plasmid pMUTIN4. Moreover, we found that the rapC mutants exhibited a marked decrease in iturin A production, suggesting that the rapC gene was closely related to the iturin A production
Prediction of the Caspian Sea level using ECMWF seasonal forecasts and reanalysis
No full textThis article is made available through the Brunel Open Access Publishing Fund. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and
reproduction in any medium, provided the original author(s) and the
source are credited.The hydrological budget of the Caspian Sea (CS) is investigated using the European Centre for Medium-Range Weather Forecasts interim reanalysis (ERAi) and seasonal forecast (FCST) data with the aim of predicting the Caspian Sea Level (CSL) some months ahead. Precipitation and evaporation are used. After precipitation events over the Volga River, the discharge (Volga River discharge (VRD)) follows with delays, which are parameterized. The components of the water budget from ERAi and FCSTs are integrated to obtain time series of the CSL. Observations of the CSL and the VRD are used for comparison and tuning. The quality of ERAi data is sufficiently good to calculate the time variability of the CSL with a satisfactory accuracy. Already the storage of water within the Volga Basin allows forecasts of the CSL a few months ahead, and using the FCSTs of precipitation improves the CSL forecasts. The evaporation in the seasonal forecasts is deficient due to unrealistic sea surface temperatures over the CS. Impacts of different water budget terms on the CSL variability are shown by a variety of validation tools. The importance of precipitation anomalies over the catchment of the Volga River is confirmed, but also impacts from the two southern rivers (Sefidrud and Kura River) and the evaporation over the CS become obvious for some periods. When pushing the FCSTs beyond the limits of the seasonal FCSTs to 1 year, considerable forecast skill can still be found. Validating only FCSTs by the present approach, which show the same trend as one based on a statistical method, significantly enhances the skill scores
A class of finite groups with Abelian Sylow p-subgroups
No full textIn this paper, we first determine the structure of the Sylow P-subgroup P of a finite group G containing no elements of order 2p (p > 2), and then show that the Broue Abelian Defect Groups Conjecture is true for the principal P-block of G. The result depends on the classification of finite simple groups.Mathematics, AppliedMathematicsSCI(E)中国科学引文数据库(CSCD)0ARTICLE3471-4801
Thermopower and thermoelectric power factor of Zk parafermion quantum dots
No full textAbstractUsing the conformal field theory approach to the thermoelectric characteristics of fractional quantum Hall states, previously developed in Georgiev (2015) [10], we show that the thermoelectric power factor of Coulomb-blockaded islands, realized by point contacts in Fabry–Pérot interferometers in the Zk parafermion Hall states, could give reliable signatures for distinguishing the topological orders of different quantum Hall states having identical electric properties. For example, while the conductance peak patterns in the Coulomb blockade regime for such states are practically indistinguishable for vn≪vc even at finite temperature, where vn and vc are the Fermi velocities of the neutral and charged modes respectively, the power factors PT of the corresponding states are much more sensitive to the neutral modes. In particular, the smaller r=vn/vc the bigger the asymmetries in the power factor which combined with the thermal broadening of the conductance peaks due to the neutral modes' multiplicities could give us the ultimate tool to figure out which of the competing quantum Hall universality classes are indeed realized in the experiments. We give a complete description of the power factor profiles in the Z3 and Z4 parafermion states with arbitrary number of quasiparticles localized in the bulk which could be useful for comparison with the experiments
On the Hermite and Mathieu Special Characterizations to the Logarithmic Zakharov–Kuznetsov Equations
No full textIn this paper, we find the new travelling wave solutions for several aspects of logarithmic Zakharov–Kuznetsov (ZK) equations using an efficient technique called the special function method which is composed of Hermite and Mathieu differential equations being novel and special functions. In order to illustrate the efficiency of the projected scheme, we considered four different examples with different cases, namely, logarithmic ZK (log-ZK) equation, logarithmic modified ZK (log-mZK) equation, and logarithmic ZK modified equal width (log-ZK-mEW) equation and logarithmic ZK–Benjamin–Bona–Mahony (log-ZKBBM) equation. The behaviour of the obtained results and corresponding consequences are illustrated and captured. Finally, the obtained results confirm that the considered solution procedure can be widely employed to find the solution and also capture some interesting and stimulating consequences. © 2023, The Author(s), under exclusive licence to Springer Nature India Private Limited
Sums of zeros of solutions to second order differential equations with polynomial coefficients
No full textWe consider the equation u''=P(z)u, where P(z) is a polynomial. Let zk(u), k=1, 2, ... be the zeros of a solution u(z) to that equation. Inequalities for the sums ∑k=1j |zk(u)|-1 (j=1, 2, ...) are derived. They considerably improve the previous result of the author. Some applications of the obtained bounds are also discussed. An illustrative example is presented. It shows that the suggested results are sharp
On Some Narrations in the (Post)Industrial Design Field
No full textThis text examines main narrations of the socio-cultural design field in the postindustrial period. The author starts from the creation of the definition of postindustriality. Next he explores main trends of contemporary design (DIY; eco/social design; design for all senses; design thinking, design for experience). Finally he tries to apply for the interpretation of this topic sociological categories of Jean Baudrillard and George Ritzer, eg.: ‘madness of production’, ‘immaterial consumption’, ‘hiperreality’, ‘simulacra’
Notatk i filmowe Federica Felliniego. Notatnik reżysera jako manifest poetyki twórcy
No full textThe article is devoted to a little-known film Fellini: A Director’s Notebook (1969), which is included by the author in the so-called film “notes” – essayistic, selfreferential films depicting the directors’ work on fictional movies. As the author argues, Notebook … can be viewed as a kind of manifesto of the poetics (and even “semiotics”) of the artist’s films, which expresses its attitude to issues such as relations: director-actor, author-work, and film-reality through an essayistic form. Fellini plays with his image (appearing personally on the screen) and fictionalizes the non-fictional genre, creating a kind of “fake-documentary”. The subject and the quasi-documentary form of the “notes” allow the director to demonstrate an individual approach to the relationship between the film and reality. As the author argues, Notebook… was for Fellini a kind of transitional film, developing new artistic ideas – from then on, Fellini’s fictional works will be more and more essayistic, artificial, heterogeneous, and fragmentary.The article is devoted to a little-known film Fellini: A Director’s Notebook (1969), which is included by the author in the so-called film “notes” – essayistic, selfreferential films depicting the directors’ work on fictional movies. As the author argues, Notebook … can be viewed as a kind of manifesto of the poetics (and even “semiotics”) of the artist’s films, which expresses its attitude to issues such as relations: director-actor, author-work, and film-reality through an essayistic form. Fellini plays with his image (appearing personally on the screen) and fictionalizes the non-fictional genre, creating a kind of “fake-documentary”. The subject and the quasi-documentary form of the “notes” allow the director to demonstrate an individual approach to the relationship between the film and reality. As the author argues, Notebook… was for Fellini a kind of transitional film, developing new artistic ideas – from then on, Fellini’s fictional works will be more and more essayistic, artificial, heterogeneous, and fragmentary
Privacy enhancing technologies : protocol verification, implementation and specification
No full textIn this thesis, we present novel methods for verifying, implementing and specifying protocols. In particular, we focus properties modeling data protection and the protection of privacy. In the first part of the thesis, the author introduces protocol verification and presents a model for verification that encompasses so-called Zero-Knowledge (ZK) proofs. These ZK proofs are a cryptographic primitive that is particularly suited for hiding information and hence serves the protection of privacy. The here presented model gives a list of criteria which allows the transfer of verification results from the model to the implementation if the criteria are met by the implementation. In particular, the criteria are less demanding than the ones of previous work regarding ZK proofs. The second part of the thesis contributes to the area of protocol implementations. Hereby, ZK proofs are used in order to improve multi-party computations. The third and last part of the thesis explains a novel approach for specifying data protection policies. Instead of relying on policies, this approach relies on actual legislation. The advantage of relying on legislation is that often a fair balancing is introduced which is typically not contained in regulations or policies.In dieser Arbeit werden neue Methoden zur Verifikation, Implementierung und Spezifikation im von Protokollen vorgestellt. Ein besonderer Fokus liegt dabei auf Datenschutz-Eigenschaften und dem Schutz der Privatsph¨are. Im ersten Teil dieser Arbeit geht der Author auf die Protokoll- Verifikation ein und stellt ein Modell zur Verifikation vor, dass sogenannte Zero-Knowledge (ZK) Beweise enth¨alt. Diese ZK Beweise sind ein kryptographisches primitiv, dass insbesondere zum Verstecken von Informationen geeignet ist und somit zum Schutz der Privatsph¨are dient. Das hier vorgestellte Modell gibt eine Liste von Kriterien, welche eine Implementierung der genutzten kryptographischen Primitive erf¨ullen muss, damit die verifikationen im Modell sich auf Implementierungen ¨ubertragen lassen. In Bezug auf ZK Beweise sind diese Kriterien sch¨acher als die vorangegangener Arbeiten. Der zweite Teil der Arbeit wendet sich der Implementierung von Protokollen zu. Hierbei werden dann ZK Beweise verwendet um sichere Mehrparteienberechnungen zu verbessern. Im dritten und letzten Teil der Arbeit wird eine neuartige Art der Spezifikation von Datenschutz-Richtlinien erl¨autert. Diese geht nicht von Richtlinien aus, sondern von der Rechtsprechung. Der Vorteil ist, dass in der Rechtsprechung konkrete Abw¨agungen getroffen werden, die Gesetze und Richtlinien nicht enthalten
- …
