1,720,959 research outputs found
ANTI-INFLAMMATORY ACTIVITY OF HMG CO A REDUCTASE INHIBITORS: A COMPARATIVE STUDY
Get PDFObjective: The purpose of the present study was to reveal the potential pleotropic anti-inflammatory activity of two hmg-co Reductase inhibitors, Rosuvastatin and atorvastatin in comparison with standard drug diclofenac.Methods: The method used for screening anti-inflammatry activity was In vitro human red blood cell membrane stabilization method. Results: The results of our study revealed that atorvastatin and rosuvastatin showed effective in vitro(500mcg/ml) anti-inflammatory activity when compared with the standard drug diclofenac.Conclusion: The rosuvastatin was more potent anti-inflamatory drug when compared with atorvastatin.Â
FORMULATION AND IN VITRO EVALUATION OF SELF NANO EMULSIFYING DRUG DELIVERY SYSTEM OF QUERCETIN FOR ENHANCEMENT OF ORAL BIOAVAILABILITY
Get PDFObjective: The study aims to develop a Self-nanoemulsifying drug delivery system (SNEDDS) of Quercetin to enhance its oral bioavailability.
Methods: In the present study, Quercetin was formulated into SNEDDS using various oils, surfactants, and co-surfactants. The developed formulations were subjected to various studies like drug content analysis, droplet size and thermodynamic stability, and in vitro drug release studies.
Results: From the screening of oils, surfactant and cosurfactant, the combination of Triacetin as oil phase, Tween 20 as surfactant and Ethanol as co-surfactant was selected for the development of SNEDDS of Quercetin. The composition of the formulation was optimized using pseudo ternary phase diagram. The optimized formulation has been evaluated and found to have good physical stability and improved in vitro drug release.
Conclusion: A stable SNEDDS of Quercetin was developed, and results indicated substantial enhancement in the dissolution of the drug when formulated as a self-nano emulsifying drug delivery system, indicating its potential to enhance oral solubility and bioavailability of the drug
STATINS INDUCED NEPHROTOXICITY: A DOSE DEPENDENT STUDY IN ALBINO RATS
Get PDFObjective: The objective of the study was to evaluate the dose dependent effects of atorvastatin on rat kidneys.
Methods: The selected doses of atorvastatin were administered orally for 17 days to rats. The statin induced nephrotoxicity was accessed by carrying out renal function tests and is by renal histopathology analysis. Measurements of antioxidant enzymes were also carried out to check whether atorvastatin at the selected dose level interfere with any of the antioxidant system. Atorvastatin at three doses 5mg/kg, 10mg/kg and 20mg/kg was tested.
Results: Higher doses of atorvastatin exhibits considerable degree of renal toxicity in rats, while the low doses do not interfere with renal functions in rats. Atorvastatin at all the tested dose levels not interfere with cellular anti-oxidant mechanisms.
Conclusions: Atorvastatin at 20mg/kg exhibit significant toxicity on rat kidneys. Atorvastatin at the selected dose level does not interfere with any of the antioxidant mechanisms. It indicated that atorvastatin not cause oxidative stress induced renal damage and rhabdomyolysis may be the reason for renal damage induced by atorvastatin at the high dose level
DEVELOPMENT AND VALIDATION OF A NEW RP HPLC ANALYTICAL METHOD FOR THE DETERMINATION OF ETODOLAC SUCCINIC ACID CO-CRYSTALS IN SPIKED RABBIT PLASMA
Get PDFObjective: The aim of the study was to develop and validate a novel and sensitive HPLC method for the determination of Etodolac content in Etodolac succinic acid co-crystals in spiked rabbit plasma.
Methods: Chromatographic separation was achieved on an Eclipse C18 column (4.6 mm,100 mm, 3.5 μm spherical particles) using acetonitrile: methanol: acetic acid (100%) (50:49:1) as the mobile phase at a flow rate of 0.8 ml/min and monitored at 278 nm. Tinidazole was used as the internal standard. The run time was 6 min. The method was validated to fulfill International Conference on Harmonisation (ICH) guidelines, which included specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision and robustness.
Results: The calibration curve was linear over the concentration range from 2.5 to 15 μg/ml, and the lower limit of detection was 0.3700 μg/ml. and lower limit of quantification was 1.121μg/ml for determination in spiked rabbit plasma. The accuracy and precision of the method were within the acceptable limit of±2% at the lower limit of quantification.
Conclusion: A simple, sensitive, rapid and reproducible RP-HPLC method was developed with short runtime and less flow rate. Statistical analysis of the method proved that this method is suitable for the estimation of Etodolac in Co-crystalin plasma. Hence this method can be employed in the routine assay of the Etodolac Succinic acid co-crystals
ISOLATION OF PHYTOCONSTITUENT, IN VITRO ANTICANCER STUDY IN HELA AND MCF-7 CELL LINES AND MOLECULAR DOCKING STUDIES OF POTHOS SCANDENS LINN
Get PDFObjective: This study aims to isolate an active phytoconstituent from ethanolic leaf extract of Pothos scandens Linn., to evaluate in vitro anticancer activity, and to carry out molecular docking studies of the isolated phytoconstituent.
Methods: The bioactive constituent 1,1’-(4,5-dihydroxy benzene-1,2-diyl) bisoct-7-en-1-one, a phenolic compound, was isolated by using chromatographic methods and the structure was elucidated by various spectroscopic techniques. In vitro anticancer activity was evaluated against HeLa and MCF 7 cell lines. The viability of cells was evaluated by direct observation of cells by an Inverted phase-contrast microscope and by the MTT assay method. IC50 was calculated using the linear regression model.
Results: The results of anticancer studies revealed that different concentrations of the ethanolic extract of leaves of Pothos scandens Linn. exhibited cytotoxic activity against HeLa and MCF 7 cell lines with IC50 of 22.9 and 18.32 μg/ml, respectively. The anticancer potential of the plant was revalidated by in silico molecular docking study with Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, PDB ID: 4AG8) using Discovery studio 2018. Results of the docking study showed that the ligand exhibited strong interaction with the VEGFR2 kinase with significant binding energy.
Conclusion: Pothos scandens linn. can be used for the isolation of potent anticancer agents
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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