1,721,040 research outputs found
Preliminary multi-omics analysis of BDNF effects in an ischemic stroke model of cerebral organoids
No full textAnnual Meeting 2025 of the PhD Course in Cellular and Molecular Biotechnologie
Are We What We Eat? Impact of Diet on the Gut–Brain Axis in Parkinson’s Disease
Get PDFParkinson’s disease is characterized by motor and non-motor symptoms, such as defects in the gut function, which may occur before the motor symptoms. To date, there are therapies that can improve these symptoms, but there is no cure to avoid the development or exacerbation of this disorder. Dysbiosis of gut microbiota could have a crucial role in the gut–brain axis, which is a bidirectional communication between the central nervous system and the enteric nervous system. Diet can affect the microbiota composition, impacting gut–brain axis functionality. Gut microbiome restoration through probiotics, prebiotics, synbiotics or other dietary means could have the potential to slow PD progression. In this review, we will discuss the influence of diet on the bidirectional communication between gut and brain, thus supporting the hypothesis that this disorder could begin in the gut. We also focus on how food-based therapies might then have an influence on PD and could ameliorate non-motor as well as motor symptoms
Targeting Neuronal Senescence: A High-Throughput In Vitro Strategy to Identify Anti-Aging Compounds
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Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Protective effects of exosomes-derived from human amniotic fluid stem cells intranasally administered in a unilaterally lesioned 6-OHDA mouse model
No full textParkinson’s disease (PD) is a progressively debilitating neurodegenerative disorder that leads to motor and cognitive dysfunction. To date, clinical treatment can only ameliorate symptoms, but cannot efficiently protect dopaminergic neurons. Several reports have demonstrated that human amniotic fluid stem cells (hAFSCs) provide protective effects on brain injury. Stem cells communicate with cells through secreted exosomes, the present study aimed to explore whether exosomes derived from hAFSCs-conditioned media may exert a protective effect in a mouse model of PD. The exosomes were characterized, and then labeled with PKH 26 (in order to visualize them fluorescently into the brain) and administered intranasally (i.n.) in a mouse model of PD, obtained by unilateral 6-OHDA injection into the striatum. We decided to use intranasal administration because it is one of the less invasive routes of administration and because through i.n. administration exosomes are able to cross the BBB. Behavioral tests (gait analyses, cylinder test, elevated body swing test and apomorphine) and tyrosine hydroxylase (TH) expression in the nigro-striatal structures were performed. We then analyzed the inflammatory pathway, through Iba1 immunofluorescence, Proteome Profiler Array and ELISA kits for IL-8 (GRO/KC), NFKB and so on. Notably, it was found that the exosomes relieved apomorphine-induced asymmetric rotation and improved behavioral performances in 6-OHDA mice, reduced substantia nigra dopaminergic neuron loss and inflammation. These results demonstrated that hAFSCs-exosomes can cross the BBB and can represent a potential approach for PD
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